A dual affinity tag on the 64-kDa Nlt1p subunit allows the rapid characterization of mutant yeast oligosaccharyl transferase complexes

Archives of Biochemistry and Biophysics

Volumn 338, Issue 1, 1997, Pages 1-6

  Pathak, Rahul ^a   Imperiali, Barbara ^a  

^a California Institute of Technology   (United States)

Author keywords

affinity purification; affinity tagging; N linked glycosylation; oligosaccharyl transferase; Saccharomyces cerevisiae

Indexed keywords

ANTISERUM; FUNGAL PROTEIN; GLYCOPROTEIN; MEMBRANE PROTEIN; OLIGOSACCHARIDE; POLYCLONAL ANTIBODY; POLYPEPTIDE; TRANSFERASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL MEMBRANE; ENZYME PURIFICATION; ENZYME SUBUNIT; EUKARYOTE; FUNGUS MUTANT; GLYCOSYLATION; NONHUMAN; PRIORITY JOURNAL; ROUGH ENDOPLASMIC RETICULUM; SACCHAROMYCES CEREVISIAE; STRAIN DIFFERENCE; YEAST;

EUKARYOTA; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0030918349     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1006/abbi.1996.9812     Document Type: Article

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