Overexpression and purification of human XPA using a baculovirus expression system

Protein Expression and Purification

Volumn 19, Issue 1, 2000, Pages 1-11

  Hermanson, Ingrid L ^a   Turchi, John J ^a  

^a WRIGHT STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACULOVIRUS; CISPLATIN; COMPLEX FORMATION; DNA DAMAGE; DNA REPAIR; ELECTROPHORETIC MOBILITY; EXPRESSION VECTOR; HYBRID PROTEIN; INSECT CELL; PROTEIN DNA BINDING; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; XERODERMA PIGMENTOSUM;

ANIMALIA; EUKARYOTA; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0034123191     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1224     Document Type: Article

References (30)

1. Wood R. D. DNA repair in eukaryotes. Ann. Rev. Biochem. 65:1996;135-167.
2. De Weerd-Kastelein E. A., Keijzer W., Bootsma D. Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization. Nature New Biol. 238:1972;80-83.
3. Tanaka K., Satokata I., Ogita Z., Uchida T., Okada Y. Molecular cloning of a mouse DNA repair gene that complements the defect of group-A xeroderma pigmentosum. Proc. Natl. Acad. Sci. USA. 86:1989;5512-5516.
4. Cleaver J., States J. The DNA damage-recognition problem in human and other eukaryotic cells: The XPA damage binding protein. Biochem. J. 328:1997;1-12.
5. Robins P., Jones C. J., Biggerstaff M., Lindahl T., Wood R. D. Complementation of DNA repair in xeroderma pigmentosum group A cell extracts by a protein with affinity for damaged DNA. EMBO J. 10:1991;3913-3921.
6. Layher S. K., Cleaver J. E. Quantification of XPA gene expression levels in human and mouse cell lines by competitive RT-PCR. Mutat. Res. 383:1997;9-19.
7. Tanaka K., Miura K., Satokata I., Miyamoto I., Yoshida M., Satoh Y., Kondo S., Yasui A., Okayama H., Okada Y. Analysis of a human DNA excision repair gene involved in group A xeroderma pigmentosum and containing a zinc-finger. Nature. 348:1990;73-76.
8. Jones C. J., Wood R. D. Preferential binding of the xeroderma pigmentosum group A complementing protein to damaged DNA. Biochemistry. 32:1993;12096-12104.
9. Park C. H., Sancar A. Reconstitution of mammalian excision repair activity with mutant cell-free extracts and XPAC and ERCC1 proteins expressed in Escherichia coli. Nucleic Acids Res. 21:1993;5110-5116.
10. Ariza R. R., Keyse S. M., Moggs J. G., Wood R. D. Reversible protein phosphorylation modulates nucleotide excision repair of damaged DNA by human cell extracts. Nucleic Acids Res. 24:1996;433-440.
11. Henricksen L. A., Wold M. S. Replication protein A mutants lacking phosphorylation sites for p34cdc2 kinase support DNA replication. J. Biol. Chem. 269:1994;24203-24208.
12. Turchi J. J., Henkels K. Human Ku autoantigen binds cisplatin-damaged DNA but fails to stimulate human DNA-activated protein kinase. J. Biol. Chem. 271:1996;13861-13867.
13. Turchi J. J., Li M., Henkels K. M. Cisplatin-DNA binding specificity of calf high-mobility group 1 protein. Biochemistry. 35:1996;2992-3000.
14. Patrick S. M., Turchi J. J. Human replication protein A preferentially binds duplex DNA damaged with cisplatin. Biochemistry. 37:1998;8808-8815.
15. Patrick S. M., Henkels K. M., Turchi J. J. High-mobility group 1 protein inhibits helicase catalyzed displacement of cisplatin-damaged DNA. Biochim. Biophys. Acta. 1354:1997;279-290.
16. Turchi J. J., Patrick S. M., Henkels K. M. Mechanism of DNA-dependent protein kinase inhibition by cis-diamminedichloroplatinum(II)-damaged DNA. Biochemistry. 36:1997;7586-7593.
17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
18. Henkels K. M., Turchi J. J. Induction of apoptosis in cisplatin-sensitive and -resistant human ovarian cancer cell lines. Cancer Res. 57:1997;4488-4492.
19. Wood R. D., Robins P., Lindahl T. Complementation of the xeroderma pigmentosum DNA repair defect in cell-free extracts. Cell. 53:1988;97-106.
20. Miura N., Miyamoto I., Asahina H., Satokata I., Tanaka K., Okada Y. Identification and characterization of xpac protein, the gene product of the human XPAC (xeroderma pigmentosum group A complementing) gene. J. Biol. Chem. 266:1991;19786-19789.
21. Park C. H., Sancar A. Formation of a ternary complex by human XPA, ERCC1, and ERCC4(XPF) excision repair proteins. Proc. Natl. Acad. Sci. USA. 91:1994;5017-5021.
22. Li L., Peterson C. A., Lu X., Legerski R. J. Mutations in XPA that prevent association with ERCC1 are defective in nucleotide excision repair. Mol. Cell Biol. 15:1995;1993-1998.
23. Li L., Elledge S. J., Peterson C. A., Bales E. S., Legerski R. J. Specific association between the human DNA repair proteins XPA and ERCC1. Proc. Natl. Acad. Sci. USA. 91:1994;5012-5016.
24. Park C. H., Mu D., Reardon J. T., Sancar A. The general transcription-repair factor TFIIH is recruited to the excision repair complex by the XPA protein independent of the TFIIE transcription factor. J. Biol. Chem. 270:1995;4896-4902.
25. Lee S. H., Kim D. K., Drissi R. Human xeroderma pigmentosum group A protein interacts with human replication protein A and inhibits DNA replication. J. Biol. Chem. 270:1995;21800-21805.
26. He Z., Henricksen L. A., Wold M. S., Ingles C. J. RPA involvement in the damage-recognition and incision steps of nucleotide excision repair. Nature. 374:1995;566-569.
27. Kuraoka I., Morita E. H., Saijo M., Matsuda T., Morikawa K., Shirakawa M., Tanaka K. Identification of a damaged-DNA binding domain of the XPA protein. Mutat. Res. 362:1996;87-95.
28. Satokata I., Tanaka K., Okada Y. Molecular basis of group A xeroderma pigmentosum: A missense mutation and two deletions located in a zinc finger consensus sequence of the XPAC gene. Hum. Genet. 88:1992;603-607.
29. Gunz D., Hess M., Naegeli H. Recognition of DNA adducts by human nucleotide excision repair: Evidence for a thermodynamic probing mechanism. J. Biol. Chem. 271:1996;25089-25098.
30. Patrick S. M., Turchi J. J. Replication protein A (RPA) binding to duplex cisplatin-damaged DNA is mediated through the generation of single-stranded DNA. J. Biol. Chem. 274:1999;14972-14978.