Single-step trypsin cleavage of a fusion protein to obtain human insulin and its C peptide

European Journal of Biochemistry

Volumn 236, Issue 2, 1996, Pages 656-661

  Jonasson, Per ^a   Nilsson, Joakim ^a   Samuelsson, Elisabet ^a   Moks, Tomas ^a   Ståhl, Stefan ^a   Uhlén, Mathias ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

affinity chromatography; fusion protein; proinsulin; refolding; staphylococcal protein A

Indexed keywords

C PEPTIDE; HYBRID PROTEIN; PROINSULIN;

ARTICLE; CELL INCLUSION; ESCHERICHIA COLI; GENETIC ENGINEERING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEQUENCE ANALYSIS;

EID: 0029876212     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.00656.x     Document Type: Article

References (29)

1. Brosius, J. & Holy, A. (1984) Regulation of ribosomal RNA promotors with a synthetic lac operator, Proc. Natl Acad. Sci. USA 81, 6929-6933.
2. Cole, R. D. (1967) Sulfitolysis, Methods Enzymol. 11, 206-208.
3. Cousens, L. S., Shuster, J. R., Gallegos, C., Ku, L., Stempien, M. M., Urdea, M. S., Sanchez-Pescador, R., Taylor, A. & Tekamp-Olson, P. (1987) High level expression of proinsulin in the yeast, Saccharomyces cerevisiae, Gene (Amst.) 61, 265-275.
4. Frank, B. H., Pettee, J. M., Zimmerman, R. E. & Burck, P. J. (1981) The production of human proinsulin and its transformation to human insulin and C peptide, in Peptides: synthesis, structure and function, proceedings of the seventh american peptide symposium (Rich, D. H. & Gross, E., eds) pp. 729-738, Pierce Chemical Company, Rockford, IL.
5. Guo, L.-H., Stepién, P. P., Tso, J. Y., Brousseau, R., Narang, S., Thomas, D. Y. & Wu, R. (1984) Synthesis of human insulin gene. Construction of expression vectors for fused proinsulin production in Escherichia coli, Gene (Amst.) 29, 251-254.
6. Hansson, M., Ståhl, S., Hjorth, R., Uhlén, M. & Moks, T. (1994) Single-step recovery of a secreted recombinant protein by expanded bed adsorption, Biotechnology 12, 285-288.
7. Hultman, T., Ståhl, S., Hornes, E. & Uhlén, M. (1989) Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support, Nucleic Acids Res. 17, 4937-4946.
8. Johansson, B.-L., Sjöberg, S. & Wahren, J. (1992) The influence of human C peptide on renal function and glucose utilization in type 1 (insulin-dependent) diabetic patients. Diabetologia 35, 121-128.
9. Johansson, B.-L., Kernell, A., Sjöberg, S. & Wahren, J. (1993) Influence of combined C peptide and insulin administration on renal function and metabolic control in diabetes type 1, J. Clin. Endocrinol. Metab. 77, 976-981.
10. Kang, Y. & Yoon, J.-W. (1991) Development of a high-expression vector (pYK10-9) of human proinsulin gene. Biotechnol. Lett. 13, 755-760.
11. Kang, Y. & Yoon, J.-W. (1994) Effect of modification of connecting peptide of proinsulin on its export, J. Biotechnol. 36, 45-54.
12. Kemmler, W., Peterson, J. D. & Steiner, D. F. (1971) Studies on the conversion of proinsulin to insulin, J. Biol. Chem. 246, 6786-6791.
13. Löwenadler, B., Jansson, B., Paleus, S., Holmgren, E., Nilsson, B., Moks, T., Palm, G., Josephson, S., Philipson, L. & Uhlén, M. (1987) A gene fusion system for generating antibodies against short peptides, Gene (Amst.) 58, 87-97.
14. Moks, T., Abrahmsén, L., Österlöf, B., Josephson, S., Östling, M., Enfors, S.-O., Persson, I., Nilsson, B. & Uhlén, M. (1987) Large-scale affinity purification of human insulin-like growth factor I from culture medium of Escherichia coli, Biotechnology 5, 379-382.
15. Murby, M., Cedergren, L., Nilsson, J., Nygren, P.-A., Hammarberg, B., Nilsson, B., Enfors, S.-O. & Uhlén, M. (1991) Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy, Biotechnol. Appl. Biochem. 14, 336-346.
16. Murby, M., Nguyen, T. N., Binz, H., Uhlén, M. & Ståhl, S. (1994) Production and recovery of recombinant proteins of low solubility, in Separations for biotechnology (Pyle, D. L., ed.) vol. 3, pp. 336-344, Boockcraft Ltd, Bath.
17. Nilsson, B., Moks, T., Jansson, B., Abrahmsén, L., Elmblad, A., Holmgren, E., Henrichson, C., Jones, T. A. & Uhlén, M. (1987) A synthetic IgG-binding domain based on staphylococcal protein A, Prot. Eng. 1, 107-113.
18. Nygren, P.Å., Ståhl, S. & Uhlén, M. (1994) Engineering proteins to facilitate bioprocessing, Trends Biotechnol. 12, 184-188.
19. Öberg, U., Rundström, G., Grönlund, H., Uhlén, M. & Nygren, P-Å. (1994) Intracellular production and renaturation from inclusion bodies of a scFv fragment fused to a serum albumin binding affinity tail, in Proc. 6th Eur. Congr. Biotechnol. (Alberghina, L., Frontali, L. & Sensi, P., eds) pp. 179-182. Elsevier Science B. V., Amsterdam, The Netherlands.
20. Rudolph, R. (1995) Successful protein folding on an industrial scale, in Principles and practice of protein folding (Cleland, J. L. & Craik, C. S., eds) pp. 283-298. John Wiley and Sons Inc., New York.
21. Rüther, U. (1982) pUR 250 allows rapid chemical sequencing of both DNA strands of inserts, Nucleic Acids Res. 10, 5765-5772.
22. Samuelsson, E., Moks, T., Nilsson, B. & Uhlén, M. (1994) Enhanced in vitro refolding of insulin-like growth factor I using a solubilizing fusion partner, Biochemistry 33, 4207-4211.
23. Shen, S.-H. (1984) Multiple joined genes prevent product degradation in Escherichia coli, Proc. Natl Acad. Sci. USA 81, 4627-4631.
24. Ståhl, S., Sjölander, A., Nygren, P-Å., Berzins, K., Perlmann, P. & Uhlén, M. (1989) A dual expression system for the generation, analysis and purification of antibodies to a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA, J. Immunol. Meth. 124, 43-52.
25. Uhlén, M., Nilsson, B., Guss, B., Lindberg, M., Gatenbeck, S. & Philipson, L. (1983) Gene fusion vectors based on staphylococcal protein A, Gene (Amst.) 23, 369-378.
26. Uhlén, M., Forsberg, G., Moks, T., Hartmanis, M. & Nilsson, B. (1992) Fusion proteins in biotechnology, Curr. Opin. Biotechnol. 3, 363-369.
27. Wang, M., Scott, W. A., Rao, R., Udey, J., Conner, G. E. & Brew, K. (1989) Recombinant bovine α-lactalbumin obtained by limited proteolysis of a fusion protein expressed at high levels in Escherichia coli, J. Biol. Chem. 264, 21 116-21 121.
28. Williams, D. C., Van Frank, R. M., Muth, W. L. & Burnett, J. P. (1982) Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins, Science 215, 687-689.
29. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of M13mp18 and pUC19 vectors, Gene (Amst.) 33, 103-119.