Vector-derived expression of recombinant rat interleukin-6

Protein Expression and Purification

Volumn 7, Issue 3, 1996, Pages 269-274

  Braciak, T A ^a   Northemann, W ^b   Chong, D K ^a   Schroeder, J A ^a   Gauldie, J ^a  

^a MCMASTER UNIVERSITY   (Canada)

^b ELIAS Entwicklungslabor ^*  (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRIDAE; ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HUMAN ADENOVIRUS; MURINAE; ORYCTOLAGUS CUNICULUS;

EID: 0030152136     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0038     Document Type: Article

References (9)

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3. 3. Frorath, B., Abney, C. C., Berthold, H., Scanarini, M., and Northemann, W. (1992) Production of recombinant rat interleukin-6 in Escherichia coli using a novel highly efficient expression vector pGEX-3T. Biotechniques 12, 558-563.
4. 4. Northemann, W., Braciak, T. A., Hattori, M., Lee, F., and Fey, G. H. (1989) Structure of the rat interleukin 6 gene and its expression in macrophage-derived cells. J. Biol. Chem. 264, 16072-16082.
5. 5. Smith, D. B., and Johnson, K. S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
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7. 7. Mauch, L., Seibler, J., Haubruck, H., Cook, N. J., Abney, C. C., Berthold, H., Wirbelauer, C., Liedvogel, B., Scherbaum, W. A., and Northemann, W. (1993) Baculovirus-mediated expression of human 65 kDa and 67 kDa glutamic acid decarboxylases in SF9 insect cells and their relevance in diagnosis of insulin-dependent diabetes mellitus. J. Biochem. 113, 699-704.
8. 8. Braciak, T. A., Mittal, S. K., Graham, F. L., Richards, C. D., and Gauldie, J. (1993) Construction of recombinant human type 5 adenoviruses expressing rodent IL-6 genes: An approach to investigate in vivo cytokine function. J. Immunol. 151, 5145-5153.
9. 9. Gauldie, J., Richards, C., Harnish, D., Lansdorp, P., and Baumann, H. (1987) Interferon beta 2/B cell stimulatory factor 2 shares identity with monocyte-derived hepatocyte-stimulatory factor and regulates the major acute phase protein response in liver cells. Proc. Natl. Acad. Sci. USA 84, 7251-7255.