Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: Efficient regeneration of the fusion protein and selective cleavage with trypsin

Protein Science

Volumn 5, Issue 3, 1996, Pages 456-467

  Chen, Guo Qiang ^a   Gouaux, J Eric ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

bacteriorhodopsin; Escherichia coli; maltose binding protein; membrane protein overexpression; membrane protein refolding; selective trypsin cleavage; synthetic gene

Indexed keywords

BACTERIORHODOPSIN; HYBRID PROTEIN; MALTOSE BINDING PROTEIN;

ARTICLE; CELL REGENERATION; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; SOLUBILIZATION;

EID: 0030001914     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050307     Document Type: Article

References (52)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. 1993. Current protocols in molecular biology. New York: John Wiley & Sons, Inc. pp 1.8.1-1.8.8; 11.20-11.28.
2. Bauer U, Hildebrandt V, Dencher NA, Wrede P. 1992. In vitro synthesis of bacterio-opsin: Integration into microsomal membranes. Biochem Biophys Res Commun 187:1480-1485.
3. Bayley H, Huang KS, Radhakrishnan R, Ross AH, Takagaki Y, Khorana HG. 1981. Site of attachment of retinal in bacteriorhodopsin. Proc Natl Acad Sci USA 78:2225-2229.
4. Braiman MS, Stern LJ, Chao BH, Khorana HG. 1987. Structure-function studies on bacteriorhodopsin. IV. Purification and renaturation of bacterio-opsin polypeptide expressed in Escherichia coli. J Biol Chem 262:9271-9276.
5. Chen GQ, Choi I, Ramachandran B, Gouaux JE. 1994. Total gene synthesis: Novel single-step and convergent strategies applied to the construction of a 779 base pair bacteriorhodopsin gene. J Am Chem Soc 116: 8799-8800.
6. De Grip WJ. 1982. Purification of bovine rhodopsin over concanavalin A-Sepharose. Methods Enzymol 81:197-201.
7. Devereux J, Haeberli P, Smithies O. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387-395.
8. Ferrando E, Schweiger U, Oesterhelt D. 1993. Homologous bacterio-opsin-encoding gene expression via site-specific vector integration. Gene 125:41-47.
9. Gale AJ, Schimmel P. 1995. Isolated RNA binding domain of a class I tRNA synthetase. Biochemistry 34:8896-8903.
10. Grisshammer R, Duckworth R, Henderson R. 1993. Expression of a rat neurotensin receptor in Escherichia coli. Biochem J 295:571-576.
11. Henderson R, Baldwin JM, Ceska TA, Zemlin F, Beckmann E, Downing KH. 1990. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. J Mol Biol 213:899-929.
12. Hildebrandt V, Polakowski F, Buldt G. 1991. Purple fission yeast: Over-expression and processing of the pigment bacteriorhodopsin in Schizosaccharomyces pombe. Photochem Photobiol 54:1009-1016.
13. Hiraoka O, Anaguchi H, Yamasaki K, Fukunaga R, Nagata S, Ota Y. 1994. Ligand binding domain of granulocyte colony-stimulating factor receptor. J Biol Chem 269:22412-22419.
14. Huang KS, Bayley H, Khorana HG. 1980. Delipidation of bacteriorhodopsin and reconstitution with exogenous phospholipid. Proc Natl Acad Sci USA 77:323-327.
15. Huang KS, Bayley H, Liao MJ, London E, Khorana HG. 1981. Refolding of an integral membrane protein. Denaturation, renaturation and reconstitution of intact bacteriorhodopsin and two proteolytic fragments. J Biol Chem 256:3802-3809.
16. Kahn TW, Engelman DM. 1992. Bacteriorhodopsin can be refolded from two independently stable transmembrane helices and the complementary five-helix fragment. Biochemistry 51:6144-6151.
17. Karnik SS, Nassal M, Doi T, Jay E, Sgaramella V, Khorana HG. 1987. Structure-function studies on bacteriorhodopsin. J Biol Chem 262:9255-9263.
18. Kellermann OK, Ferenci T. 1982. Maltose binding protein from Escherichia coli. Methods Enzymol 90:459-463.
19. Khorana HG. 1993. Two light-transducing membrane proteins: Bacteriorhodopsin and the mammalian rhodopsin. Proc Natl Acad Sci USA 90:1166-1171.
20. Khorana HG, Gerber GE, Herlihy WC, Gray CP, Anderegg RJ, Nehei K, Biemann K. 1979. Amino acid sequence of bacteriorhodopsin. Proc Natl Acad Sci USA 76:5046-5050.
21. Ko YH, Thomas PJ, Delannoy MR, Pedersen PL. 1993. The cystic fibrosis transmembrane conductance regulator. J Biol Chem 268:24330-24338.
22. Krebs MP, Hauss T, Heyn MP, RajBhandary UL, Khorana HG. 1991. Expression of bacterioopsin gene in Halobacterium halobium using a multi-copy plasmid. Proc Natl Acad Sci USA 88:859-863.
23. Krebs MP, Khorana HG. 1993. Mechanism of light-dependent proton translocation by bacteriorhodopsin. J Bacteriol 175:1555-1560.
24. Lanyi JK. 1993. Proton translocation mechanism and energetics in the light-driven pump bacteriorhodopsin. Biochim Biophys Acta 1183:241-261.
25. Liao MJ, London E, Khorana HG. 1983. Regeneration of the native bacteriorhodopsin structure from two chymotryptic fragments. J Biol Chem 258:9949-9955.
26. London E, Khorana HG. 1982. Denaturation and renaturation of bacteriorhodopsin in detergents and lipid-detergent mixtures. J Biol Chem 257: 7003-7011.
27. MacKenzie D, Arendt A, Hargrave P, McDowell JH, Molday RS. 1984. Localization of binding sites for carboxyl terminal specific anti-rhodopsin monoclonal antibodies using synthetic peptides. Biochemistry 23:6544-6549.
28. Maina CV, Riggs PD, Grandea AG III, Slatko BE, Moran LS, Tagliamonte JA, McReynolds LA, Guan CD. 1988. An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74:365-373.
29. Mathies RA, Lin SW, Ames JB, Pollard WT. 1991. From femtoseconds to biology: Mechanism of bacteriorhodopsin's light-driven proton pump. Annu Rev Biophys Biophys Chem 20:491-518.
30. Matsudaira P. 1987. Sequence from picomole quantities of proteins electroblotted onto polyvinylidine difluoride membranes. J Biol Chem 262: 10035-10038.
31. Miercke LJW, Betlach MC, Mitra AK, Shand RF, Fong SK, Stroud RM. 1991. Wild-type and mutant bacteriorhodopsins D85N, D96N, and R82Q: Purification to homogeneity, pH dependence of pumping, and electron diffraction. Biochemistry 30:3088-3098.
32. Milder SJ, Thorgeirsson TE, Miercke LJW, Stroud RM, Kliger DS. 1991. Effects of detergent environments on the photocycle of purified monomeric bacteriorhodopsin. Biochemistry 30:1751-1761.
33. Muccio DD, DeLucas LJ. 1985. Isolation of detergent-solubilized monomers of bacteriorhodopsin by size-exclusion high-performance liquid chromatography. J Chromatography 326:243-250.
34. Nambiar KP, Stackhouse J, Presnell SR, Benner SA. 1987. Expression of bovine pancreatic ribonuclease A in Escherichia coli. Eur J Biochem 163:67-11.
35. Ni B, Chang M, Duschl A, Lanyi J, Needleman R. 1990. An efficient system for the synthesis of bacteriorhodopsin in Halobacterium halobium. Gene 90:169-172
36. Oesterhelt D, Stoeckenius W 1971. Rhodopsin-like protein from the purple membrane of Halobacterium halobium. Nature New Biology 233:149-152.
37. Oprian DD, Molday RS, Kaufman RJ, Khorana HG. 1987. Expression of a synthetic bovine rhodopsin gene in monkey kidney cells. Proc Natl Acad Sci USA 84:8874-8878.
38. Ovchinnikov YA, Abdulaev NG, Feigina MY, Kiselev AV, Lobanov NA. 1979. The structural basis of the functioning of bacteriorhodopsin: An overview. FEBS Lett 100:219-224.
39. Pompejus M, Friedrich K, Teufel M, Fritz HJ. 1993. High-yield production of bacteriorhodopsin via expression of a synthetic gene in Escherichia coli. Eur J Biochem 211:27-35.
40. Prodromou C, Pearl LH. 1992. Recursive PCR: A novel technique for total gene synthesis. Protein Eng 5:827-829.
41. Quinlan RA, Moir RD, Stewart M. 1989. Expression in Escherichia coli of fragments of glial fibrillary acidic protein: Characterization, assembly properties and paracrystal formation J Cell Sci 93:71-83.
42. Rehorek M, Heyn MP. 1979. Binding of all-trans-retinal to the purple membrane. Evidence for cooperativity and determination of the extinction coefficient Biochemistry 18:4977-4983.
43. Robyt JF, White BJ. 1987. Biochemical techniques: Theory and practice. Monterey, California: Brooks/Cole. pp 235-236.
44. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning. New York: Cold Spring Harbor Laboratory Press, pp 18.47-18.55.
45. Sandhu GS, Aleff RA, Kline BC. 1992. Dual asymmetric PCR: One-step construction of synthetic genes. BioTechniques 12:14-16.
46. Schertler GFX 1992. Overproduction of membrane proteins. Curr Op Struct Biol 2:534-544.
47. Shand RF, Miercke LJW, Mitra AK, Fong SK, Stroud RM, Betlach MC. 1991. Wild-type and mutant bacterioopsins D85N, D96N, and R82Q: High-level expression in Escherichia coli. Biochemistry 30:3082-3088.
48. Sonar S, Patel N, Fischer W, Rothschild KJ. 1993. Cell-free synthesis, functional refolding, and spectroscopic characterization of bacteriorhodopsin, an integral membrane protein. Biochemistry 32:13777-13781.
49. Wald G, Brown PK. 1953. The molar extinction of rhodopsin. J Gen Physiol 37:189-200.
50. Wildenauer D, Khorana HG. 1977. The preparation of lipid-depleted bacteriorhodopsin. Biochim Biophys Acta 466:315-324.
51. Yamaguchi N, Jinbo Y, Arai M, Koyama K. 1993 Visualization of the morphology of purple membrane surfaces by monoclonal antibody techniques. FEBS Lett 324:287-292.
52. Ye QZ, Johnson LL, Baragi V. 1992. Gene synthesis and expression in E. coli for PUMP, a human matrix metalloproteinase. Biochem Biophys Res Commun 186:143-149.