Chain initiation on the soraphen-producing modular polyketide synthase from Sorangium cellulosum

Chemistry and Biology

Volumn 8, Issue 12, 2001, Pages 1197-1208

  Wilkinson, Christopher J ^a   Frost, Elizabeth J ^a   Staunton, James ^a   Leadlay, Peter F ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Acyltransferase; Chain initiation; Erythromycin; Polyketide; Soraphen

Indexed keywords

BACTERIA (MICROORGANISMS); ILEX COOKII; POLYANGIUM CELLULOSUM; SACCHAROPOLYSPORA ERYTHRAEA; SORANGIUM; UNCULTURED ACTINOMYCETE;

HETEROCYCLIC COMPOUND; MULTIENZYME COMPLEX; POLYKETIDE SYNTHASE; SORAPHEN A;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; MYXOCOCCALES; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE; RNA TRANSLATION;

AMINO ACID SEQUENCE; HETEROCYCLIC COMPOUNDS; KINETICS; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; MYXOCOCCALES; PEPTIDE CHAIN INITIATION; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY; SUPPORT, NON-U.S. GOV'T;

EID: 0035542855     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(01)00087-4     Document Type: Article

References (60)

1. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea
2. Modular organization of genes required for complex polyketide biosynthesis
3. 6-Deoxyerythronolide B synthase 2 from Saccharopolyspora erythraea. Cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme
4. The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin
5. Organization of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: Analysis of the enzymatic domains in the modular polyketide synthase
6. Production of a novel polyketide through the construction of a hybrid polyketide synthase
7. Identification and characterization of the niddamycin polyketide synthase genes from Streptomyces caelestis
8. Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis
9. The biosynthetic gene cluster for the 26-membered ring polyene macrolide pimaricin - A new polyketide synthase organization encoded by two subclusters separated by functionalization genes
10. Identification of a gene cluster for antibacterial polyketide-derived antibiotic biosynthesis in the nystatin producer Streptomyces noursei ATCC 11455
11. Amphotericin biosynthesis in Streptomyces nodosus: Deductions from analysis of polyketide synthase and late genes
12. The pipecolate-incorporating enzyme for the biosynthesis of the immunosuppressant rapamycin - Nucleotide sequence analysis, disruption and heterologous expression of rapP from Streptomyces hygroscopicus
13. Genetic contributions to understanding polyketide synthases
14. Identification of a 60-kb region of the chromosome of Pseudomonas fluorescens NCIB-10586 required for the biosynthesis of pseudomonic acid (mupirocin)
15. Metabolic diversity in myxobacteria: Identification of the myxalamid and the stigmatellin biosynthetic gene cluster of Stigmatella aurantiaca Sg a15 and a combined polyketide-(poly)peptide gene cluster from the epothilone producing strain Sorangium cellulosum So ce 90
16. New lessons of combinatorial biosynthesis from myxobacteria - The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1
17. The biosynthetic gene cluster for the microtubule-stabilizing agents epothilones A and B from Sorangium cellulosum So ce 90
18. Isolation and characterization of the epothilone biosynthetic gene cluster from Sorangium cellulosum
19. Combinatorial biosynthesis of polyketides and nonribosomal peptides
20. Engineering broader specificity into an antibiotic-producing polyketide synthase
21. Novel erythromycins from a recombinant Saccharopolyspora erythraea strain NRRL 2338 pIG1 - I. Fermentation, isolation and biological activity
22. Polyketide synthesis in vitro on a modular polyketide synthase
23. A chain initiation factor common to both modular and aromatic polyketide synthases
24. Biosynthesis of the ansamycin antibiotic rifamycin: Deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699
25. The loading module of rifamycin synthetase is an adenylation-thiolation didomain with substrate tolerance for substituted benzoates
26. The Escherichia coli malonyl-CoA:acyl carrier protein transacylase at 1.5-angstrom resolution - Crystal structure of a fatty acid synthase component
27. Alteration of the substrate specificity of the malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase domain of the multifunctional fatty acid synthase by mutation of a single arginine residue
28. Novel avermectins produced by mutational biosynthesis
29. Substrate specificity of the loading didomain of the erythromycin polyketide synthase
30. A Sorangium cellulosum (Myxobacterium) gene cluster for the biosynthesis of the macrolide antibiotic soraphen A - Cloning, characterization, and homology to polyketide synthase genes from actinomycetes
31. A hybrid modular polyketide synthase obtained by domain swapping
32. Construction of new vectors for high-level expression in actinomycetes
33. A cluster of bacterial genes for anaerobic benzene ring biodegradation
34. Benzoate-coenzyme A ligase, encoded by badA, is one of three ligases able to catalyze benzoyl-coenzyme A formation during anaerobic growth of Rhodopseudomonas palustris on benzoate
35. Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus
36. Limited proteolysis and active site studies of the first multienzyme component of the erythromycin-producing polyketide synthase
37. The polyketide synthase (PKS) of aspyrone biosynthesis - Evidence for the enzyme bound intermediates from incorporation studies with N-acetylcysteamine thioesters in intact cells of Aspergillus melleus
38. Macrolide biosynthesis. 7. Incorporation of polyketide chain elongation intermediates into methymycin
39. The potential role of fatty acid initiation in the biosynthesis of the fungal aromatic polyketide aflatoxin B-1
40. Erythromycin biosynthesis - Highly efficient incorporation of polyketide chain elongation intermediates into 6-deoxyerythronolide B in an engineered Streptomyces host
41. A plant-like biosynthesis of benzoyl-CoA in the marine bacterium 'Streptomyces maritimus'
42. Cloning, sequencing and analysis of the enterocin biosynthesis gene cluster from the marine isolate 'Streptomyces maritimus': Evidence for the derailment of an aromatic polyketide synthase
43. The loading domain of the erythromycin polyketide synthase is not essential for erythromycin biosynthesis in Saccharopolyspora erythraea
44. Precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase
45. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli
46. Origin and true nature of the starter unit for the rapamycin polyketide synthase
47. Evidence for a double-helical structure for modular polyketide synthases
48. The parallel and convergent universes of polyketide synthases and nonribosomal peptide synthetases
49. High efficiency transformation of E. coli by high voltage electroporation
50. Improved M13 phage cloning vectors and host strains - Nucleotide sequences of the M13MP18 and pUC19 vectors
51. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp
52. Transformation of Streptomyces erythraeus
53. Analysis of seven genes from the eryAI/eryK region of the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea
54. Evaluating precursor-directed biosynthesis towards novel erythromycins through in vitro studies on a bimodular polyketide synthase
55. Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-CoA:acyl carrier protein transacylase domains in modular polyketide synthases
56. Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage