Precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase

Science

Volumn 277, Issue 5324, 1997, Pages 367-369

  Jacobsen, John R ^a   Hutchinson, C Richard ^b   Cane, David E ^c   Khosla, Chaitan ^d  

^a Stanford University   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c BROWN UNIVERSITY   (United States)

^d STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT; ERYTHROMYCIN; ERYTHROMYCIN DERIVATIVE; MACROLIDE; POLYKETIDE; SYNTHETASE;

ARTICLE; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME SUBSTRATE; GENETIC ENGINEERING; PRIORITY JOURNAL; SYNTHESIS;

AMINO ACID SUBSTITUTION; BACILLUS CEREUS; BINDING SITES; CATALYSIS; CYCLIZATION; ERYTHROMYCIN; MICROBIAL SENSITIVITY TESTS; MULTIENZYME COMPLEXES; MUTAGENESIS, SITE-DIRECTED; SACCHAROPOLYSPORA; STREPTOMYCES; TRANSFORMATION, GENETIC;

EID: 0030875774     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5324.367     Document Type: Article

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54. +: 432.2807. Found: 432.2810.
55. We thank M. McPherson for mass spectrometry of 6dEB derivatives. Research in our laboratories was supported by grants from NIH (CA66736 to C.K., GM22172 to D.E.C., and GM31925 to C.R.H.), an NSF Young Investigator Award (to C.K.), and a David and Lucile Packard Fellowship for Science and Engineering (to C.K.). J.R.J. is a recipient of a National Institute of General Medical Sciences postdoctoral fellowship (1 F32 GM18590-01).