Combinatorial biosynthesis of polyketides and nonribosomal peptides

Current Opinion in Chemical Biology

Volumn 5, Issue 2, 2001, Pages 159-164

  Staunton, James ^a   Wilkinson, Barrie ^b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b BIOTICA TECHNOLOGY LTD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ERYTHROMYCIN; ISOENZYME; METHYMYCIN; MEVINOLIN; OLEANDOMYCIN; PEPTIDE; PEPTIDE SYNTHASE; PIKROMYCIN; POLYKETIDE; POLYKETIDE SYNTHASE; RAPAMYCIN;

COMBINATORIAL CHEMISTRY; DRUG SCREENING; GENE TECHNOLOGY; GENETIC ENGINEERING; METHODOLOGY; PROTEIN SYNTHESIS; REVIEW;

EID: 0035313411     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(00)00185-X     Document Type: Review

References (46)

1. Genetic contributions to understanding polyketide synthases
2. Polyketide synthesis: Prospects for hybrid antibiotics
3. Harnessing the biosynthetic code: Combinations, permutations and mutations
4. Design and application of multi modular peptide synthetases
5. Engineering broader specificity into an antibiotic producing polyketide synthase
6. Acyltransferase domain substitutions in the erythromycin polyketide synthase yield novel erythromycin derivatives
7. Gain of function mutagenesis of the erythromycin polyketide synthase. 2. Engineered biosynthesis of an eight-membered ring tetraketide lactone
8. Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage
9. Manipulation of macrolide ring size by directed mutagenesis of a modular polyketide synthase
10. Engineering of a minimal modular polyketide synthase and targeted alteration of the stereospecificity of polyketide chain extension
11. Novel erythromycins from a recombinant Saccharopolyspora erythraea strain NRRL 2338 pIG1. I. Fermentation, isolation and biological activity
12. Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel 'unnatural' natural products
13. A multiplasmid approach to preparing large libraries of polyketides
14. Production of a novel polyketide through the construction of a hybrid polyketide synthase
15. Formation of functional heterologous complexes using subunits from the picromycin, erythromycin and oleandomycin polyketide synthases
16. Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis
17. Lovastatin nonaketide synthase catalyses an intramolecular Diels-Alder reaction of a substrate analogue
18. Knowledge-base design of bimodular and trimodular polyketide synthases based on domain and module swaps: A route to simple statin analogues
19. Engineered biosynthesis of novel polyketides: Influence of a downstream enzyme on the catalytic specificity of a minimal aromatic polyketide synthase
20. Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits
21. Deciphering the mechanism of the assembly of aromatic polyketides by a bacterial polyketide synthase
22. Disruption of an aromatase/cyclase from the oxytetracycline gene cluster of Streptomyces rimosus results in production of novel polyketides with shorter chain lengths
23. Ectopic expression of the minimal whiE polyketide synthase generates a library of aromatic polyketides of diverse sizes and shapes
24. A chain initiation factor common to both modular and aromatic polyketide synthases
25. Conversion of a β ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with glutamine
26. Cloning, sequencing and analysis of the enterocin biosynthesis gene cluster from the marine isolate 'Streptomyces maritimus': Evidence for the derailment of an aromatic polyketide synthase
27. Alternative modular polyketide synthase expression controls macrolactone structure
28. The parallel and convergent universes of polyketide synthases and nonribosomal peptide synthetases
29. Initiation, elongation and termination strategies in polyketide and polypeptide antibiotic biosynthesis
30. Aminoacyl-CoAs as probes of condensation domain selectivity in nonribosomal peptide synthesis
31. Acyl-CoA hydrolysis by the high molecular weight protein 1 subunit of yersiniabactin synthetase: Mutational evidence for a cascade of four acyl-enzyme intermediates during hydrolytic editing
32. Peptide cyclization catalysed by the thioesterase domain of tyrocidine synthetase
33. Rational design of peptide antibiotics by targeted replacement of bacterial and fungal domains
34. Construction of hybrid peptide synthetases by module and domain fusions
35. Dipeptide formation on engineered hybrid peptide synthetases
36. Construction and in vitro analysis of a new bi-modular polypeptide synthetase for synthesis of N-methylated acyl peptides
37. The specificity conferring code of adenylation domains in nonribosomal peptide synthetases
38. Predictive, structure based model of amino acid recognition by nonribosomal peptide adenylation domains
39. Polyketide synthase gene coupled to the peptide synthetase module involved in the biosynthesis of the cyclic heptapeptide microcystin
40. Structural organisation of microcystin biosynthesis in Microcystis aeruginosa PCC7806: An integrated peptide-polyketide synthetase system
41. The biosynthetic gene cluster for the antitumor drug bleomycin from Streptomyces verticillus ATCC15003 supporting functional interactions between nonribosomal peptide synthetases and a polyketide synthase
42. The mycosubtilin of Bacillus subtilis ATCC6633: A multifunctional hybrid between a peptide synthetase, an amino transferase and a fatty acid synthase
43. New lessons for combinatorial biosynthesis from myxobacteria. The myxothiazol biosynthetic gene cluster of Stigmatella aurantiaca DW4/3-1
44. Novel features in a combined polyketide synthase/nonribosomal peptide synthetase: The myxalamid biosynthetic gene cluster of the myxobacterium Stigmatella aurantiaca Sg a15
45. The myxochelin iron transport regulon of the myxobacterium Stigmatella aurantiaca Sg a15
46. Production of the antitumor drug epirubicin (4′-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius