The loading domain of the erythromycin polyketide synthase is not essential for erythromycin biosynthesis in Saccharopolyspora erythraea

Microbiology

Volumn 144, Issue 2, 1998, Pages 543-553

  Pereda, Ana ^a   Summers, Richard G ^a   Stassi, Diane L ^a   Ruan, Xiaoan ^a   Katz, Leonard ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

Loading domain; Polyketide synthase; Streptomyces

Indexed keywords

ACYL CARRIER PROTEIN; ACYLTRANSFERASE; ERYTHROMYCIN; POLYKETIDE; SYNTHETASE;

ARTICLE; BIOSYNTHESIS; DRUG SYNTHESIS; FUNGUS MUTANT; NONHUMAN; PRIORITY JOURNAL; RECOMBINANT DNA TECHNOLOGY; SACCHAROPOLYSPORA;

FUNGI; SACCHAROPOLYSPORA; SACCHAROPOLYSPORA ERYTHRAEA; STREPTOMYCES;

EID: 0031888940     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-144-2-543     Document Type: Article

References (40)

1. Aigle, B., Schneider, D., Morilhat, C., Vandewiele, D., Daty, A., Holl, A.-C., Simonet, J.-M. & Decaris, B. (1996). An amplifiable and deletable locus of Streptomyces ambofaciens RP181110 contains a very large gene homologous to polyketide synthase genes. Microbiology 142, 2815-2824.
2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990). Basic local alignment search tool. J Mol Biol 215, 403-410.
3. Aparicio, J. F., Caffrey, P., Marsden, A. F. A., Stauton, J. & Leadlay, P. F. (1994). Limited proteolysis and active-site studies of the first multienzyme component of the erythromycin-producing polyketide synthase. J Biol Chem 269, 8524-8528.
4. Bedford, D., Jacobsen, J. R., Luo, G., Cane, D. E. & Khosla, C. (1996). A functional chimeric modular polyketide synthase generated via domain replacement. Chem Biol 3, 827-831.
5. Bevitt, D. J, Cortes, J., Haydock, S. F. & Leadlay, P. F. (1992). 6-Deoxyerythronolide-B synthase 2 from Saccharopolyspora erythraea. Cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme. Eur J Biochem 204, 39-49.
6. Bibb, M. J., White, J., Ward, J. M. & Janssen, G. R. (1994). The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythraea is translated in the absence of a conventional ribosome-binding site. Mol Microbiol 14, 533-545.
7. Caffrey, P., Bevitt, D. J., Staunton, J. & Leadlay, P. F. (1992). Identification of DEBS1, DEBS2 and DEBS3, the multienzyme polypeptides of the erythromycin-producing polyketide synthase from Saccharopolyspora erythraea. FEBS Lett 304, 225-228.
8. Cortes, J., Haydock, S. F., Roberts, G. A., Bevitt, D. J. & Leadlay, P. F. (1990). An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea. Nature 348, 176-178.
9. Devereux, J., Haeberli, P. & Smithies, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12, 387-395.
10. Dewitt, J. P. (1985). Evidence for a sex factor in Streptomyces erythreus. J Bacteriol 164, 969-971.
11. Donadio, S. & Katz, L. (1992). Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea. Gene 111, 51-60.
12. Donadio, S., Staver, M., McAlpine, J. B., Swanson, S. J. & Katz, L. (1991). Modular organization of genes required for complex polyketide biosynthesis. Science 252, 675-679.
13. Donadio, S., McAlpine, J. B., Shelden, P. J., Jackson, M. & Katz, L. (1993). An erythromycin analog produced by reprogramming polyketide synthesis. Proc Natl Acad Sci USA 90, 7119-7123.
14. Hopwood, D. A. & Sherman, D. H. (1990). Molecular genetics of polyketides and its comparison to fatty acid biosynthesis. Annu Rev Genet 24, 37-66.
15. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. & Schempf, H. (1985). Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich : John Innes Foundation.
16. Hsieh, Y.-J. & Kolattukudy, P. E. (1994). Inhibition of erythromycin synthesis by disruption of malonyl-coenzyme A decarboxylase gene eryM in Saccharopolyspora erythraea. J Bacteriol 176, 714-724.
17. Hutchinson, C. R. & Fujii, I. (1995). Polyketide synthase gene manipulation: a structure-function approach in engineering novel antibiotics. Annu Rev Microbiol 49, 201-238.
18. Jacoben, J. R., Hutchinson, C. R., Cane, D. E. & Khosla, C. (1997). Precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase. Science 277 , 367-369.
19. Kao, C. M., Katz, L. & Khosla, C. (1994). Engineered biosynthesis of a complete macrolactone in a heterologous host. Science 265, 509-512.
20. Katz, L. & Donadio, S. (1993). Polyketide synthesis: prospect for hybrid antibiotics. Annu Rev Microbiol 47, 875-912.
21. Kuhstoss, S., Huber, M., Turner, J. R., Paschal, J. W. & Rao, N. (1996). Production of a novel polyketide through the construction of a hybrid polyketide synthase. Gene 183, 231-236.
22. McAlpine, J. B., Tuan, J. S., Brown, D. P., Grebner, K. D., Whittern, D. N., Buko, A. & Katz, L. (1987). New antibiotics from genetically engineered actinomycetes. J Antibiot 40, 1115-1122.
23. Marsden, A. F. A., Caffrey, P., Aparicio, J. F., Loughran, M. S., Staunton, J. & Leadlay, P. F. (1994). Stereospecific acyl transfers on the erythromycin-producing polyketide synthase. Science 263, 378-380.
24. Mathur, M. & Kolattukudy, P. E. (1992). Molecular cloning and sequencing of the gene for mycocerosic acid synthase, a novel fatty acid elongating multifunctional enzyme, from Mycobacterium tuberculosis var. bovis Bacillus Calmette-Guerin. J Biol Chem 267, 19388-19395.
25. Molnar, I., Aparicio, J. F., Haydock, S. F., Khaw, L. E., Schwecke, T. & O'Hagan, D. (1993). Biosynthesis of fatty acid and polyketide metabolites. Nat Prod Rep 10, 593-624.
26. Olinynyk, I., Brown, M. J. B., Cortes, J., Staunton, J. & Leadlay, P. F. (1996). A hybrid modular polyketide synthase obtained by domain swapping. Chem Biol 3, 833-839.
27. Osakabe, Y., Osakabe, K., Kawai, S., Katayama, Y. & Morohoshi, N. (1995). Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis. Plant Mol Biol 28, 1133-1141.
28. Pieper, R., Ebert-Khosla, S., Cane, D. & Khosla, C. (1996a). Erythromycin biosynthesis: kinetic studies on a fully active modular polyketide synthase using natural and unnatural substrates. Biochemistry 35, 2054-2060.
29. Pieper, R., Kao, C., Khosla, C., Luo, G. & Cane, D. E. (1996b). Specificity and versatility in erythromycin biosynthesis. Chem Soc Rev 25, 297-302.
30. Ruan, X., Stassi, D., Lax, S. & Katz, L. (1997a). A second type-I PKS gene cluster isolated from Streptomyces hygroscopicus ATCC 29253, a rapamycin-producing strain. Gene 184, 1-9.
31. Ruan, X., Pereda, A., Stassi, D., Zeidner, D., Summers, R. G., Jackson, M., Shivakumar, A., Kakavas, S., Staver, M. J., Donadio, S. & Katz, L. (1997b). Acyltransferase domain substitutions in the erythromycin polyketide synthase yield novel erythromycin derivatives. J Bacteriol 179, 6416-6425.
32. Ruby, C. L. & Danis, S. J. (1992). Complex organization of the Streptomyces avermitilis genes encoding the avermectin polyketide synthase. Gene 115, 119-125.
33. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
34. Schwecke, T., Aparicio, J. F., Molnar, I., Konig, A., Khaw, L. E., Haydock, S. F., Oliynyk, M., Caffrey, P., Cortes, J. B., Lester, J. B., Bohm, G. A., Staunton, J. & Leadlay, P. F. (1995). The biosynthethic gene cluster for the polyketide immunosuppressant rapamycin. Proc Natl Acad Sci USA 92, 7839-7843.
35. Swan, D. G., Rodriguez, A. M., Vilches, C., Mendez, C. & Salas, J. A. (1994). Characterization of a Streptomyces antibioticus gene encoding a type I polykedde synthase which has an unusual coding sequence. Mol Gen Genet 242, 358-362.
36. Tsukamoto, N., Chuck, J.-A., Luo, G., Kao, C. M., Khosla, C. & Cane, D. E. (1996). 6-Deoxyerythronolide B synthase 1 is specifically acylated by a diketide intermediate at the betaketoacyl-acyl carrier protein synthase domain of module 2. Biochemistry 35, 15244-15248.
37. Vara, J. A., Lewandowska-Skarbek, M., Wang, Y.-G., Donadio, S. & Hutchinson, C. R. (1989). Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus). J Bacteriol 171, 5872-5881.
38. Weber, J. M. & Losick, R. (1988). The use of a chromosome integration vector to map erythromycin resistance and production genes in Saccharopolyspora erythraea (Streptomyces erythreus). Gene 68, 173-180.
39. Weber, J. M., Leung, J. O., Swanson, S. J., Idler, K. B. & McAlpine, J. B. (1991). An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea. Science 252, 114-117.
40. Yamamoto, H., Maurer, K. H. & Hutchinson, C. R. (1986). Transformation of Streptomyces erythreus. J Antibiot 39, 1304-1313.