Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)

Proceedings of the National Academy of Sciences of the United States of America

Volumn 115, Issue 21, 2018, Pages 5444-5449

  Singh, Digvijay ^a   Mallon, John ^a   Poddar, Anustup ^a   Wang, Yanbo ^a   Tippana, Ramreddy ^a   Yang, Olivia ^a   Bailey, Scott ^a   Ha, Taekjip ^a,b,c  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Johns Hopkins University   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

CRISPR; CRISPR Cas12a; CRISPR Cpf1; Gene editing; Single molecule

Indexed keywords

DNA; ENDONUCLEASE; GUANINE; GUIDE RNA; PROTEIN CPF1; UNCLASSIFIED DRUG; BACTERIAL DNA; BACTERIAL PROTEIN; BACTERIAL RNA; CPF1 NUCLEASE, ACIDAMINOCOCCUS; PROTEIN BINDING; SINGLE STRANDED DNA;

ACIDAMINOCOCCUS; AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; CRISPR-CAS9 SYSTEM; DNA CLEAVAGE; FLUORESCENCE ANALYSIS; FRANCISELLA NOVICIDA; LACHNOSPIRACEAE; NONHUMAN; ORTHOLOGY; PH; PRIORITY JOURNAL; PROTEIN DNA BINDING; BACTERIAL GENOME; CHEMISTRY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONFORMATION; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR MODEL;

ACIDAMINOCOCCUS; BACTERIAL PROTEINS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DNA CLEAVAGE; DNA, BACTERIAL; DNA, SINGLE-STRANDED; ENDONUCLEASES; GENOME, BACTERIAL; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; RNA, BACTERIAL; RNA, GUIDE;

EID: 85047307013     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1718686115     Document Type: Article

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