A highly specific SpCas9 variant is identified by in vivo screening in yeast

Nature Biotechnology

Volumn 36, Issue 3, 2018, Pages 265-271

  Casini, Antonio ^a   Olivieri, Michele ^a   Petris, Gianluca ^a   Montagna, Claudia ^a   Reginato, Giordano ^a   Maule, Giulia ^a   Lorenzin, Francesca ^a   Prandi, Davide ^a   Romanel, Alessandro ^a   Demichelis, Francesca ^a   Inga, Alberto ^a   Cereseto, Anna ^a  

^a UNIVERSITY OF TRENTO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

EFFICIENCY; GENES;

GENOMIC LOCUS; MEDIAN RESIDUAL; RANDOM MUTATION; STREPTOCOCCUS PYOGENES; TARGET ACTIVITY; TARGET SITES; WILD TYPES; YEAST-BASED ASSAYS;

YEAST;

B LYMPHOCYTE RECEPTOR; CASPASE 9; DNA (CYTOSINE 5) METHYLTRANSFERASE 1; GUIDE RNA; TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 6; VASCULOTROPIN A;

ARTICLE; ASSAY; GENE LOCUS; IN VIVO STUDY; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; STREPTOCOCCUS PYOGENES; WILD TYPE; CRISPR CAS SYSTEM; ENZYME SPECIFICITY; ENZYMOLOGY; GENE EDITING; GENETICS;

CRISPR-ASSOCIATED PROTEIN 9; CRISPR-CAS SYSTEMS; GENE EDITING; MUTATION; RNA, GUIDE; STREPTOCOCCUS PYOGENES; SUBSTRATE SPECIFICITY;

EID: 85042931802     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.4066     Document Type: Article

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