High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

Nature Protocols

Volumn 2, Issue 1, 2007, Pages 31-34

  Gietz, R Daniel ^a   Schiestl, Robert H ^b  

^a UNIVERSITY OF MANITOBA   (Canada)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LITHIUM ACETATE; MACROGOL; PLASMID DNA; SINGLE STRANDED DNA; ACETIC ACID DERIVATIVE; MACROGOL DERIVATIVE;

ARTICLE; CELL CULTURE; CELL GROWTH; CELL TRANSFORMATION; CENTRIFUGATION; CULTURE MEDIUM; CULTURE TECHNIQUE; GENETIC SELECTION; HEAT SHOCK; INCUBATION TEMPERATURE; INCUBATION TIME; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SPECTROPHOTOMETRY; TEMPERATURE SENSITIVITY; YEAST CELL; CYTOLOGY; GENETIC PROCEDURES; GENETIC TRANSFORMATION; GENETICS; HEAT;

SACCHAROMYCES CEREVISIAE;

ACETATES; DNA, SINGLE-STRANDED; GENETIC TECHNIQUES; HEAT; POLYETHYLENE GLYCOLS; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

EID: 34347206860     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.13     Document Type: Article

References (11)

1. Ito, H., Fukuda, Y., Murata, K. & Kimura, A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168 (1983).
2. Schiestl, R.H. & Gietz, R.D. High efficiency transformation of intact yeast cells using single-stranded nucleic acids as carrier. Curr. Genet. 16, 339-346 (1989).
3. Gietz, R.D., Schiestl, R.H., Willems, A.R. & Woods, R.A. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11, 355-360 (1995).
4. Gietz, R.D. & Woods, R.A. Yeast transformation. in Methods in Enzymology, Guide to Yeast Genetics and Cell Biology, Parts B and C, Vol. 350 (eds. Guthrie, C. & Fink, G.R.) 87-96 (Academic Press, San Diego, CA, 2001).
5. Linske-O'Connell, L.I., Sherman, F. & McLendon, G. Stabilizing amino acid replacements at position 52 in yeast iso-1-cytochrome c: in vivo and in vitro effects. Biochemistry 34, 7094-7102 (1995).
6. Yamamoto, T., Moerschell, L.R.P., Wakem, P., Ferguson, D. & Sherman, F. Parameters affecting the frequencies of transformation and co-transformation with synthetic oligonucleotides in yeast. Yeast 8, 935-948 (1992).
7. Gietz, R.D. & Woods, R.A. Genetic transformation of yeast. BioTechniques 30, 816-831 (2001).
8. Gietz, R.D. & Schiestl, R.H. Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat. Protocols doi: 10.1038/nprot.2007.14.
9. Gietz, R.D. & Schiestl, R.H. Large scale high efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat. Protocols doi: 10.1038/nprot.2007.15.
10. Gietz, R.D. & Schiestl, R.H. Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method. Nat. Protocols doi: 10.1038/ nprot.2007.16.
11. Rose, M.D. Isolation of genes by complementation in yeast. Methods Enzymol. 152, 481-504 (1987).