Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae

Methods in Enzymology

Volumn 533, Issue , 2013, Pages 103-131

  Stuckey, Samantha ^a   Storici, Francesca ^a  

^a GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Agarose gel electrophoresis; Colony PCR of the transformants; Concentration of the pcr product; Delitto perfetto method; Genetic locus; In vivo site directed mutagenesis; PCR amplification of the core cassette; Point mutations and random mutations; Saccharomyces cerevisiae; Transformation using DNA oligonucleotides

Indexed keywords

AGAR GEL ELECTROPHORESIS; ARTICLE; DELITTO PERFETTO SYSTEM; DNA MODIFICATION; DNA SEQUENCE; GENE FUNCTION; GENE LOCUS; GENE MUTATION; GENE SILENCING; GENETIC MANIPULATION; GENETIC MARKER; GENETIC PROCEDURES; GENOMIC INSTABILITY; HOMOLOGOUS RECOMBINATION; IN VITRO STUDY; IN VIVO STUDY; KNOCKOUT GENE; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

SACCHAROMYCES CEREVISIAE;

AGAROSE GEL ELECTROPHORESIS; COLONY PCR OF THE TRANSFORMANTS; CONCENTRATION OF THE PCR PRODUCT; DELITTO PERFETTO METHOD; GENETIC LOCUS; IN VIVO SITE-DIRECTED MUTAGENESIS; PCR AMPLIFICATION OF THE CORE CASSETTE; POINT MUTATIONS AND RANDOM MUTATIONS; SACCHAROMYCES CEREVISIAE; TRANSFORMATION USING DNA OLIGONUCLEOTIDES;

ELECTROPHORESIS, AGAR GEL; GENE KNOCKOUT TECHNIQUES; GENETIC LOCI; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SELECTION, GENETIC;

EID: 84887228800     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-420067-8.00008-8     Document Type: Chapter

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20. Explanatory chapter: PCR -Primer design.
21. Agarose Gel Electrophoresis.
22. Chemical Transformation of Yeast.
23. Colony PCR.
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