Development of an efficient genome editing tool in Bacillus licheniformis using CRISPR-Cas9 nickase

Applied and Environmental Microbiology

Volumn 84, Issue 6, 2018, Pages

  Li, Kaifeng ^a   Cai, Dongbo ^b   Wang, Zhangqian ^b   He, Zhili ^a,c   Chen, Shouwen ^a,b  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b HUBEI UNIVERSITY   (China)

^c UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

Bacillus licheniformis; CRISPR Cas9n; Deletion; Genome editing; Integration; Nattokinase production

Indexed keywords

BACTERIOLOGY; EFFICIENCY; INTEGRATION;

BACILLUS LICHENIFORMIS; CRISPR-CAS9N; DELETION; ENGINEERED STRAINS; INDUSTRIAL BACTERIA; NATTOKINASE; STRAIN IMPROVEMENT; TRANSFORMATION EFFICIENCY;

GENES;

BACTERIUM; BIOLOGICAL PRODUCTION; CHROMOSOME; ENZYME; GENE EXPRESSION; GENETIC ENGINEERING; GENOME; PROTEIN;

BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS;

BACTERIAL PROTEIN; DEOXYRIBONUCLEASE I; NATTOKINASE; SUBTILISIN;

BACILLUS LICHENIFORMIS; CRISPR CAS SYSTEM; ENZYMOLOGY; GENE EDITING; GENETICS; METABOLISM; PROCEDURES;

BACILLUS LICHENIFORMIS; BACTERIAL PROTEINS; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASE I; GENE EDITING; SUBTILISINS;

EID: 85042672913     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02608-17     Document Type: Article

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