Efficient genome editing in clostridium cellulolyticum via CRISPR-Cas9 nickase

Applied and Environmental Microbiology

Volumn 81, Issue 13, 2015, Pages 4423-4431

  Xu, Tao ^a   Li, Yongchao ^a   Shi, Zhou ^a   Hemme, Christopher ^a   Li, Yuan ^a   Zhu, Yonghua ^b   Van Nostrand, Joy D ^a   He, Zhili ^a   Zhou, Jizhong ^a,c,d  

^a UNIVERSITY OF OKLAHOMA   (United States)

^b HUNAN UNIVERSITY   (China)

^c LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^d TSINGHUA UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM;

BACTERIAL GENOMES; CLOSTRIDIUM CELLULOLYTICUM; DOUBLE STRAND BREAKS; EUKARYOTIC GENOME; HOMOLOGOUS RECOMBINATION; NONHOMOLOGOUS END JOINING; SINGLE VECTORS; STREPTOCOCCUS PYOGENES;

GENES;

BACTERIUM; BIOENERGY; ENZYME ACTIVITY; EUKARYOTE; GENETIC ENGINEERING; GENOME; PROKARYOTE; RECOMBINATION;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM CELLULOLYTICUM; EUKARYOTA; PROKARYOTA; STREPTOCOCCUS PYOGENES;

DEOXYRIBONUCLEASE I;

BACTERIUM TRANSFORMATION; CLOSTRIDIUM CELLULOLYTICUM; CRISPR CAS SYSTEM; ENZYMOLOGY; GENE TARGETING; GENETICS; HOMOLOGOUS RECOMBINATION; METABOLISM; MICROBIAL GENETICS; MOLECULAR BIOLOGY; PROCEDURES; STREPTOCOCCUS PYOGENES;

CLOSTRIDIUM CELLULOLYTICUM; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASE I; GENE TARGETING; GENETICS, MICROBIAL; HOMOLOGOUS RECOMBINATION; MOLECULAR BIOLOGY; STREPTOCOCCUS PYOGENES; TRANSFORMATION, BACTERIAL;

EID: 84930787559     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00873-15     Document Type: Article

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