A novel and efficient method for bacteria genome editing employing both CRISPR/Cas9 and an antibiotic resistance cassette

Frontiers in Microbiology

Volumn 8, Issue MAY, 2017, Pages

  Zhang, Hong ^a,b   Cheng, Qiu Xiang ^c   Liu, Ai Min ^d   Zhao, Guo Ping ^a   Wang, Jin ^a  

^a INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c Shanghai Personal Biotechnology Limited Company   (China)

^d ANHUI NORMAL UNIVERSITY   (China)

Author keywords

Antibiotic resistance cassette; CRISPR Cas9; Genome editing; Protospacer adjacent motif; Sequence independent

Indexed keywords

ANTIBIOTIC RESISTANCE; BACTERIAL GENOME; CRISPR CAS SYSTEM; ESCHERICHIA COLI; ESSENTIAL GENE; GENE EDITING; GENE INSERTION; NONHUMAN; PLASMID; POINT MUTATION;

EID: 85019765181     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2017.00812     Document Type: Article

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