High level expression of a recombinant phospholipase C from Bacillus cereus in Bacillus subtilis

Applied Microbiology and Biotechnology

Volumn 74, Issue 3, 2007, Pages 634-639

  Durban, Markus A ^a   Silbersack, Jörg ^a   Schweder, Thomas ^a   Schauer, Frieder ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Bacillus cereus; Bacillus subtilis; Cloning; Expression; Phospholipase C

Indexed keywords

BACILLUS CEREUS; BACILLUS SUBTILIS; EXPRESSION; PHOSPHOLIPASE C;

AMINO ACIDS; CLONING; DNA; ENZYMES; GENES; GENETIC ENGINEERING; STRAIN; SUBSTITUTION REACTIONS; SUBSTRATES; VECTORS;

BACTERIA;

AMINO ACID; BACTERIAL DNA; BACTERIAL ENZYME; PHOSPHOLIPASE C; RECOMBINANT ENZYME; RECOMBINANT PHOSPHOLIPASE C; UNCLASSIFIED DRUG;

AMINO ACID; BACTERIUM; DNA; ENZYME ACTIVITY; GENE EXPRESSION; PH; TRANSFORMATION;

ACIDITY; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS CEREUS; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; EXPRESSION VECTOR; GENE AMPLIFICATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; SUPERNATANT; TEMPERATURE DEPENDENCE;

BACILLUS CEREUS; BACILLUS SUBTILIS; CLONING, MOLECULAR; DNA, BACTERIAL; ENZYME STABILITY; GENE EXPRESSION; GENETIC VECTORS; HEAT; HYDROGEN-ION CONCENTRATION; PHOSPHOLIPASE C; PHOSPHORYLCHOLINE; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TRANSFORMATION, BACTERIAL;

BACILLUS CEREUS; BACILLUS SUBTILIS;

EID: 33847099547     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0712-z     Document Type: Article

References (22)

1. Anthonsen T, D'Arrigo P, Pedrocchi-Fantoni G, Secundo F, Servi S, Sundby E (1999) Phospholipids hydrolysis in organic solvents catalysed by immobilised phospholipase C. J Mol Catal B Enzym 6:125-132
2. Durban M, Bornscheuer UT (2003) An assay system for the detection of phospholipase C activity. Eur J Lipid Sci Technol 105:633-637
3. El-Sayed MY, Roberts MF (1985) Charged detergents enhance the activity of phospholipase C (Bacillus cereus) towards micellar short-chain phosphatidylcholine. Biochim Biophys Acta 831:133-141
4. Garcia de Herreros A, Dominguez I, Diaz-Meco MT, Graziani G, Cornet ME, Guddal PH, Johansen T, Moscat J (1991) Requirement of phospholipase C-catalyzed hydrolysis of phosphatidylcholine for maturation of Xenopus laevis oocytes in response to insulin and ras p21. J Biol Chem 266:6825-6829
5. Ghelardi E, Celandroni F, Salvetti S, Barsotti C, Baggiani A, Senesi S (2002) Identification and characterization of toxigenic Bacillus cereus isolates responsible for two food-poisoning outbreaks. FEMS Microbiol Lett 208:129-134
6. Guddal PH, Johansen T, Schulstad K, Little C (1989) Apparent phosphate retrieval system in Bacillus cereus. J Bacteriol 171:5702-5706
7. Haftendorn R, Ulbrich-Hofmann R (2002) Activity of phospholipase C in two-phase systems. Anal Biochem 306:144-147
8. Hergenrother PJ, Spaller MR, Haas MK, Martin SF (1995) Chromogenic assay for phospholipase C from Bacillus cereus. Anal Biochem 229:313-316
9. Johansen T, Holm T, Guddal PH, Sletten K, Haugli FB, Little C (1988) Cloning and sequencing of the gene encoding the phosphatidylcholine-preferring phospholipase C of Bacillus cereus. Gene 65:293-304
10. Kamberov E, Ivanov A (1990) Purification of phospholipase-C from Bacillus cereus by affinity chromatography on 2-(4-aminophenylsulphonyl) ethyl-cellulose. J Chromatogr 525:307-318
11. Kurioka S, Matsuda M (1976) Phospholipase C assay using p-nitrophenylphosphoryl-choline together with sorbitol and its application to studying the metal and detergent requirement of the enzyme. Anal Biochem 75:281-289
12. Little C, Otnaess A-B (1975) The metal ion dependence of phospholipase C from Bacillus cereus. Biochim Biophys Acta 391:326-333
13. Martin SF, Hergenrother PJ (1998) General base catalysis by the phosphatidylcholine-preferring phospholipase C from Bacillus cereus: the role of Glu4 and Asp55. Biochemistry 37:5755-5760
14. Mulcahy LS, Smith MR, Stacey DW (1985) Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells. Nature 313:241-243
15. Notermans S, Batt CA (1998) A risk assessment approach for food-borne Bacillus cereus and its toxins. Symp Ser Soc Appl Microbiol 84:51-61
16. Sambrook J, Fritsch EF, Manniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press
17. Seo K-H, Rhee JI (2004) High-level expression of recombinant phospholipase C from Bacillus cereus in Pichia pastoris and its characterization. Biotechnol Lett 26:1475-1479
18. Silbersack J, Jürgen B, Hecker M, Schneidinger B, Schmuck R, Schweder T (2006) An acetoin-regulated expression system of Bacillus subtilis. Appl Microbiol Biotechnol (in press). DOI 10.1007/s00253-006-0549- 5
19. Tan CA, Roberts MF (1998) Engineering of the nonspecific phospholipase C from Bacillus cereus: replacement of glutamic acid-4 by alanine results in loss of interfacial catalysis and enhanced phosphomonoesterase activity. Biochemistry 37:4275-4279
20. Tan CA, Hehir MJ, Roberts MF (1997) Cloning, overexpression, refolding, and purification of the nonspecific phospholipase C from Bacillus cereus. Protein Expr Purif 10:365-372
21. Toora S, Singh G (1990) Factors affecting production and activity of phospholipase C by Yersinia enterocolitica. J Appl Bacteriol 69:113-118
22. Wu SC, Yeung JC, Duan Y, Ye R, Szarka SJ, Habibi HR, Wong SL (2002) Functional production and characterization of a fibrin-specific single-chain antibody fragment from Bacillus subtilis: effects of molecular chaperones and a wall-bound protease on antibody fragment production. Appl Environ Microbiol 68:3261-3269