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Volumn 6, Issue , 2016, Pages

A CRISPR-Cas9 assisted non-homologous end-joining strategy for one-step engineering of bacterial genome

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL GENE; BACTERIAL GENOME; CHROMOSOME; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA END JOINING REPAIR; DNA TEMPLATE; GENE DELETION; HOMOLOGOUS RECOMBINATION; BACTERIAL CHROMOSOME; CHROMOSOME DELETION; CRISPR CAS SYSTEM; DOUBLE STRANDED DNA BREAK; ESCHERICHIA COLI; GENE TARGETING; GENETIC ENGINEERING; GENETICS; LACTOSE OPERON; PROCEDURES;

EID: 84997272235     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep37895     Document Type: Article
Times cited : (92)

References (50)
  • 1
    • 84939563369 scopus 로고    scopus 로고
    • Bacterial recombineering: Genome engineering via phage-based homologous recombination
    • Pines, G., Freed, E. F., Winkler, J. D. & Gill, R. T. Bacterial recombineering: genome engineering via phage-based homologous recombination. Acs Synth Biol 4, 1176-1185, doi: 10.1021/acssynbio.5b00009 (2015).
    • (2015) Acs Synth Biol , vol.4 , pp. 1176-1185
    • Pines, G.1    Freed, E.F.2    Winkler, J.D.3    Gill, R.T.4
  • 2
    • 84926665601 scopus 로고    scopus 로고
    • A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies
    • Gu, P. F. et al. A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies. Sci Rep-Uk 5, doi: ARTN 968410.1038/srep09684 (2015).
    • (2015) Sci Rep-UK , vol.5
    • Gu, P.F.1
  • 3
    • 84958247886 scopus 로고    scopus 로고
    • Synthetic biology to access and expand nature's chemical diversity
    • Smanski, M. J. et al. Synthetic biology to access and expand nature's chemical diversity. Nat Rev Microbiol 14, 135-149, doi: 10.1038/ nrmicro.2015.24 (2016).
    • (2016) Nat Rev Microbiol , vol.14 , pp. 135-149
    • Smanski, M.J.1
  • 4
    • 84873800970 scopus 로고    scopus 로고
    • Genome-scale engineering for systems and synthetic biology
    • Esvelt, K. M. & Wang, H. H. Genome-scale engineering for systems and synthetic biology. Mol Syst Biol 9, 641, doi: 10.1038/ msb.2012.66 (2013).
    • (2013) Mol Syst Biol , vol.9 , pp. 641
    • Esvelt, K.M.1    Wang, H.H.2
  • 5
    • 84976307779 scopus 로고    scopus 로고
    • Recombineering: Genetic engineering in bacteria using homologous recombination
    • Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N. & Court, D. L. Recombineering: genetic engineering in bacteria using homologous recombination. Curr Protoc Mol Biol 106, 1 1611-1116 39, doi: 10.1002/0471142727.mb0116s106 (2014).
    • (2014) Curr Protoc Mol Biol , vol.106 , pp. 11611-111639
    • Thomason, L.C.1    Sawitzke, J.A.2    Li, X.3    Costantino, N.4    Court, D.L.5
  • 6
    • 0031924072 scopus 로고    scopus 로고
    • Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli
    • Murphy, K. C. Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli. J Bacteriol 180, 2063-2071 (1998).
    • (1998) J Bacteriol , vol.180 , pp. 2063-2071
    • Murphy, K.C.1
  • 7
    • 0031664853 scopus 로고    scopus 로고
    • A new logic for DNA engineering using recombination in Escherichia coli
    • Zhang, Y., Buchholz, F., Muyrers, J. P. & Stewart, A. F. A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet 20, 123-128, doi: 10.1038/2417 (1998).
    • (1998) Nat Genet , vol.20 , pp. 123-128
    • Zhang, Y.1    Buchholz, F.2    Muyrers, J.P.3    Stewart, A.F.4
  • 8
    • 0034612342 scopus 로고    scopus 로고
    • One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
    • Datsenko, K. A. & Wanner, B. L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. P Natl Acad Sci USA 97, 6640-6645, doi: DOI 10.1073/pnas.120163297 (2000).
    • (2000) P Natl Acad Sci USA , vol.97 , pp. 6640-6645
    • Datsenko, K.A.1    Wanner, B.L.2
  • 9
    • 84930936429 scopus 로고    scopus 로고
    • Multiplexed tracking of combinatorial genomic mutations in engineered cell populations
    • Zeitoun, R. I. et al. Multiplexed tracking of combinatorial genomic mutations in engineered cell populations. Nat Biotechnol 33, 631-637, doi: 10.1038/nbt.3177 (2015).
    • (2015) Nat Biotechnol , vol.33 , pp. 631-637
    • Zeitoun, R.I.1
  • 10
    • 84874608929 scopus 로고    scopus 로고
    • RNA-guided editing of bacterial genomes using CRISPR-Cas systems
    • Jiang, W. Y., Bikard, D., Cox, D., Zhang, F. & Marraffini, L. A. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol 31, 233-239, doi: 10.1038/nbt.2508 (2013).
    • (2013) Nat Biotechnol , vol.31 , pp. 233-239
    • Jiang, W.Y.1    Bikard, D.2    Cox, D.3    Zhang, F.4    Marraffini, L.A.5
  • 11
    • 84885610892 scopus 로고    scopus 로고
    • Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
    • Ran, F. A. et al. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell 155, 479-480, doi: 10.1016/j.cell.2013.09.040 (2013).
    • (2013) Cell , vol.155 , pp. 479-480
    • Ran, F.A.1
  • 12
    • 84949641392 scopus 로고    scopus 로고
    • Current and future prospects for CRISPR-based tools in bacteria
    • Luo, M. L., Leenay, R. T. & Beisel, C. L. Current and future prospects for CRISPR-based tools in bacteria. Biotechnol Bioeng 113, 930-943, doi: 10.1002/bit.25851 (2016).
    • (2016) Biotechnol Bioeng , vol.113 , pp. 930-943
    • Luo, M.L.1    Leenay, R.T.2    Beisel, C.L.3
  • 13
    • 84873729095 scopus 로고    scopus 로고
    • Multiplex genome engineering using CRISPR/Cas systems
    • Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819-823, doi: 10.1126/science.1231143 (2013).
    • (2013) Science , vol.339 , pp. 819-823
    • Cong, L.1
  • 14
    • 84873734105 scopus 로고    scopus 로고
    • RNA-guided human genome engineering via Cas9
    • Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823-826, doi: 10.1126/science.1232033 (2013).
    • (2013) Science , vol.339 , pp. 823-826
    • Mali, P.1
  • 15
    • 51849121635 scopus 로고    scopus 로고
    • DNA repair by nonhomologous end joining and homologous recombination during cell cycle in human cells
    • Mao, Z., Bozzella, M., Seluanov, A. & Gorbunova, V. DNA repair by nonhomologous end joining and homologous recombination during cell cycle in human cells. Cell Cycle 7, 2902-2906 (2008).
    • (2008) Cell Cycle , vol.7 , pp. 2902-2906
    • Mao, Z.1    Bozzella, M.2    Seluanov, A.3    Gorbunova, V.4
  • 16
    • 33645781346 scopus 로고    scopus 로고
    • Making ends meet: Repairing breaks in bacterial DNA by non-homologous end-joining
    • Bowater, R. & Doherty, A. J. Making ends meet: repairing breaks in bacterial DNA by non-homologous end-joining. Plos Genet 2, e8, doi: 10.1371/journal.pgen.0020008 (2006).
    • (2006) Plos Genet , vol.2 , pp. e8
    • Bowater, R.1    Doherty, A.J.2
  • 17
    • 3242881500 scopus 로고    scopus 로고
    • The cellular response to general and programmed DNA double strand breaks
    • Bassing, C. H. & Alt, F. W. The cellular response to general and programmed DNA double strand breaks. DNA Repair 3, 781-796, doi: http://dx.doi.org/10.1016/j.dnarep.2004.06.001 (2004).
    • (2004) DNA Repair , vol.3 , pp. 781-796
    • Bassing, C.H.1    Alt, F.W.2
  • 18
    • 84903362877 scopus 로고    scopus 로고
    • Programmable removal of bacterial strains by use of genome-targeting CRISPR-Cas systems
    • Gomaa, A. A. et al. Programmable removal of bacterial strains by use of genome-targeting CRISPR-Cas systems. MBio 5, e00928-00913, doi: 10.1128/mBio.00928-13 (2014).
    • (2014) MBio , vol.5 , pp. e00928-e00913
    • Gomaa, A.A.1
  • 19
    • 84983208863 scopus 로고    scopus 로고
    • Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases
    • Citorik, R. J., Mimee, M. & Lu, T. K. Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases. Nat Biotechnol 32, 1141-1145, doi: 10.1038/nbt.3011 (2014).
    • (2014) Nat Biotechnol , vol.32 , pp. 1141-1145
    • Citorik, R.J.1    Mimee, M.2    Lu, T.K.3
  • 20
    • 0034889360 scopus 로고    scopus 로고
    • Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system
    • Aravind, L. & Koonin, E. V. Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system. Genome Res 11, 1365-1374, doi: DOI 10.1101/gr.181001 (2001).
    • (2001) Genome Res , vol.11 , pp. 1365-1374
    • Aravind, L.1    Koonin, E.V.2
  • 21
    • 7444269607 scopus 로고    scopus 로고
    • Mycobacterial Ku and ligase proteins constitute a two-component NHEJ repair machine
    • Della, M. et al. Mycobacterial Ku and ligase proteins constitute a two-component NHEJ repair machine. Science 306, 683-685, doi: 10.1126/science.1099824 (2004).
    • (2004) Science , vol.306 , pp. 683-685
    • Della, M.1
  • 22
    • 67749091313 scopus 로고    scopus 로고
    • Repairing DNA double-strand breaks by the prokaryotic non-homologous end-joining pathway
    • Brissett, N. C. & Doherty, A. J. Repairing DNA double-strand breaks by the prokaryotic non-homologous end-joining pathway. Biochem Soc T 37, 539-545, doi: 10.1042/Bst0370539 (2009).
    • (2009) Biochem Soc T , vol.37 , pp. 539-545
    • Brissett, N.C.1    Doherty, A.J.2
  • 23
    • 84940106526 scopus 로고    scopus 로고
    • CRISPR-Cas9 Based engineering of actinomycetal genomes
    • Tong, Y., Charusanti, P., Zhang, L., Weber, T. & Lee, S. Y. CRISPR-Cas9 Based engineering of actinomycetal genomes. Acs Synth Biol 4, 1020-1029, doi: 10.1021/acssynbio.5b00038 (2015).
    • (2015) Acs Synth Biol , vol.4 , pp. 1020-1029
    • Tong, Y.1    Charusanti, P.2    Zhang, L.3    Weber, T.4    Lee, S.Y.5
  • 24
    • 84930197469 scopus 로고    scopus 로고
    • Targeted DNA degradation using a CRISPR device stably carried in the host genome
    • Caliando, B. J. & Voigt, C. A. Targeted DNA degradation using a CRISPR device stably carried in the host genome. Nat Commun 6, 6989, doi: 10.1038/ncomms7989 (2015).
    • (2015) Nat Commun , vol.6 , pp. 6989
    • Caliando, B.J.1    Voigt, C.A.2
  • 25
    • 34548534319 scopus 로고    scopus 로고
    • Expression of Mycobacterium tuberculosis Ku and Ligase D in Escherichia coli results in RecA and RecB-independent DNA end-joining at regions of microhomology
    • Malyarchuk, S. et al. Expression of Mycobacterium tuberculosis Ku and Ligase D in Escherichia coli results in RecA and RecB-independent DNA end-joining at regions of microhomology. DNA Repair (Amst) 6, 1413-1424, doi: 10.1016/j.dnarep.2007.04.004 (2007).
    • (2007) DNA Repair (Amst) , vol.6 , pp. 1413-1424
    • Malyarchuk, S.1
  • 26
    • 84865070369 scopus 로고    scopus 로고
    • A Programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
    • Jinek, M. et al. A Programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816-821, doi: 10.1126/science.1225829 (2012).
    • (2012) Science , vol.337 , pp. 816-821
    • Jinek, M.1
  • 27
    • 33847251953 scopus 로고    scopus 로고
    • New small nuclear RNA gene-like transcriptional units as sources of regulatory transcripts
    • Pagano, A. et al. New small nuclear RNA gene-like transcriptional units as sources of regulatory transcripts. Plos Genet 3, 174-184, doi: ARTN e110.1371/journal.pgen.0030001 (2007).
    • (2007) Plos Genet , vol.3 , pp. 174-184
    • Pagano, A.1
  • 28
    • 84892749369 scopus 로고    scopus 로고
    • Genetic screens in human cells using the CRISPR-Cas9 system
    • Wang, T., Wei, J. J., Sabatini, D. M. & Lander, E. S. Genetic screens in human cells using the CRISPR-Cas9 system. Science 343, 80-84, doi: 10.1126/science.1246981 (2014).
    • (2014) Science , vol.343 , pp. 80-84
    • Wang, T.1    Wei, J.J.2    Sabatini, D.M.3    Lander, E.S.4
  • 29
    • 84937538704 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing
    • Li, Y. F. et al. Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. Metab Eng 31, 13-21, doi: 10.1016/j.ymben.2015.06.006 (2015).
    • (2015) Metab Eng , vol.31 , pp. 13-21
    • Li, Y.F.1
  • 30
    • 84884926579 scopus 로고    scopus 로고
    • Implementation of stable and complex biological systems through recombinaseassisted genome engineering
    • Santos, C. N., Regitsky, D. D. & Yoshikuni, Y. Implementation of stable and complex biological systems through recombinaseassisted genome engineering. Nat Commun 4, 2503, doi: 10.1038/ncomms3503 (2013).
    • (2013) Nat Commun , vol.4 , pp. 2503
    • Santos, C.N.1    Regitsky, D.D.2    Yoshikuni, Y.3
  • 31
    • 84898949923 scopus 로고    scopus 로고
    • Improved seamless mutagenesis by recombineering using ccdB for counterselection
    • Wang, H. et al. Improved seamless mutagenesis by recombineering using ccdB for counterselection. Nucleic Acids Res 42, e37, doi: 10.1093/nar/gkt1339 (2014).
    • (2014) Nucleic Acids Res , vol.42 , pp. e37
    • Wang, H.1
  • 32
    • 0035810938 scopus 로고    scopus 로고
    • High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
    • Ellis, H. M., Yu, D. G., DiTizio, T. & Court, D. L. High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides. P Natl Acad Sci USA 98, 6742-6746, doi: 10.1073/pnas.121164898 (2001).
    • (2001) P Natl Acad Sci USA , vol.98 , pp. 6742-6746
    • Ellis, H.M.1    Yu, D.G.2    DiTizio, T.3    Court, D.L.4
  • 33
    • 68949161807 scopus 로고    scopus 로고
    • Programming cells by multiplex genome engineering and accelerated evolution
    • Wang, H. H. et al. Programming cells by multiplex genome engineering and accelerated evolution. Nature 460, 894-898, doi: 10.1038/nature08187 (2009).
    • (2009) Nature , vol.460 , pp. 894-898
    • Wang, H.H.1
  • 34
    • 84955464550 scopus 로고    scopus 로고
    • CRMAGE: CRISPR optimized MAGE recombineering
    • Ronda, C., Pedersen, L. E., Sommer, M. O. & Nielsen, A. T. CRMAGE: CRISPR optimized MAGE recombineering. Sci Rep 6, 19452, doi: 10.1038/srep19452 (2016).
    • (2016) Sci Rep , vol.6
    • Ronda, C.1    Pedersen, L.E.2    Sommer, M.O.3    Nielsen, A.T.4
  • 35
    • 0036228521 scopus 로고    scopus 로고
    • Engineering a reduced Escherichia coli genome
    • Kolisnychenko, V. et al. Engineering a reduced Escherichia coli genome. Genome Res 12, 640-647, doi: 10.1101/gr.217202 (2002).
    • (2002) Genome Res , vol.12 , pp. 640-647
    • Kolisnychenko, V.1
  • 36
    • 81855166264 scopus 로고    scopus 로고
    • Use of lambda Red-mediated recombineering and Cre/lox for generation of markerless chromosomal deletions in avian pathogenic Escherichia coli
    • Tuntufye, H. N. & Goddeeris, B. M. Use of lambda Red-mediated recombineering and Cre/lox for generation of markerless chromosomal deletions in avian pathogenic Escherichia coli. FEMS Microbiol Lett 325, 140-147, doi: 10.1111/j.1574-6968.2011.02421.x (2011).
    • (2011) FEMS Microbiol Lett , vol.325 , pp. 140-147
    • Tuntufye, H.N.1    Goddeeris, B.M.2
  • 37
    • 84925355124 scopus 로고    scopus 로고
    • Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system
    • Jiang, Y. et al. Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system. Appl Environ Microbiol 81, 2506-2514, doi: 10.1128/AEM.04023-14 (2015).
    • (2015) Appl Environ Microbiol , vol.81 , pp. 2506-2514
    • Jiang, Y.1
  • 38
    • 84936967101 scopus 로고    scopus 로고
    • Coupling the CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia coli
    • Pyne, M. E., Moo-Young, M., Chung, D. E. A. & Chou, C. P. Coupling the CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia coli. Appl Environ Microb 81, 5103-5114, doi: 10.1128/Aem.01248-15 (2015).
    • (2015) Appl Environ Microb , vol.81 , pp. 5103-5114
    • Pyne, M.E.1    Moo-Young, M.2    Chung, D.E.A.3    Chou, C.P.4
  • 39
    • 84944320385 scopus 로고    scopus 로고
    • The no-SCAR (scarless Cas9 assisted recombineering) system for genome editing in Escherichia coli
    • Reisch, C. R. & Prather, K. L. J. The no-SCAR (scarless Cas9 assisted recombineering) system for genome editing in Escherichia coli. Sci Rep-Uk 5, doi: ARTN 1509610.1038/srep15096 (2015).
    • (2015) Sci Rep-UK , vol.5
    • Reisch, C.R.1    Prather, K.L.J.2
  • 40
    • 84926645319 scopus 로고    scopus 로고
    • Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB-co-4HB) biosynthesis
    • Lv, L., Ren, Y. L., Chen, J. C., Wu, Q. & Chen, G. Q. Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB-co-4HB) biosynthesis. Metab Eng 29, 160-168, doi: 10.1016/j.ymben.2015.03.013 (2015).
    • (2015) Metab Eng , vol.29 , pp. 160-168
    • Lv, L.1    Ren, Y.L.2    Chen, J.C.3    Wu, Q.4    Chen, G.Q.5
  • 41
    • 84882986957 scopus 로고    scopus 로고
    • Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
    • Bikard, D. et al. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res 41, 7429-7437, doi: 10.1093/nar/gkt520 (2013).
    • (2013) Nucleic Acids Res , vol.41 , pp. 7429-7437
    • Bikard, D.1
  • 42
    • 84987875388 scopus 로고    scopus 로고
    • Editing of the Bacillus subtilis genome by the CRISPR-Cas9 system
    • Altenbuchner, J. Editing of the Bacillus subtilis genome by the CRISPR-Cas9 system. Appl Environ Microbiol, doi: 10.1128/ AEM.01453-16 (2016).
    • (2016) Appl Environ Microbiol
    • Altenbuchner, J.1
  • 43
    • 84947999145 scopus 로고    scopus 로고
    • Targeted large-scale deletion of bacterial genomes using CRISPR-nickases
    • Standage-Beier, K., Zhang, Q. & Wang, X. Targeted large-scale deletion of bacterial genomes using CRISPR-nickases. Acs Synth Biol 4, 1217-1225, doi: 10.1021/acssynbio.5b00132 (2015).
    • (2015) Acs Synth Biol , vol.4 , pp. 1217-1225
    • Standage-Beier, K.1    Zhang, Q.2    Wang, X.3
  • 44
    • 84970046200 scopus 로고    scopus 로고
    • Consequences of Cas9 cleavage in the chromosome of Escherichia coli
    • Cui, L. & Bikard, D. Consequences of Cas9 cleavage in the chromosome of Escherichia coli. Nucleic Acids Res, doi: 10.1093/nar/ gkw223 (2016).
    • (2016) Nucleic Acids Res
    • Cui, L.1    Bikard, D.2
  • 45
    • 84941285455 scopus 로고    scopus 로고
    • Bacterial CRISPR: Accomplishments and prospects
    • Peters, J. M. et al. Bacterial CRISPR: accomplishments and prospects. Curr Opin Microbiol 27, 121-126, doi: 10.1016/j. mib.2015.08.007 (2015).
    • (2015) Curr Opin Microbiol , vol.27 , pp. 121-126
    • Peters, J.M.1
  • 46
    • 84930787559 scopus 로고    scopus 로고
    • Efficient genome editing in Clostridium cellulolyticum via CRISPR-Cas9 nickase
    • Xu, T. et al. Efficient genome editing in Clostridium cellulolyticum via CRISPR-Cas9 nickase. Appl Environ Microbiol 81, 4423-4431, doi: 10.1128/AEM.00873-15 (2015).
    • (2015) Appl Environ Microbiol , vol.81 , pp. 4423-4431
    • Xu, T.1
  • 47
    • 84856034421 scopus 로고    scopus 로고
    • What limits the efficiency of double-strand break-dependent stress-induced mutation in Escherichia coli?
    • Shee, C., Ponder, R., Gibson, J. L. & Rosenberg, S. M. What limits the efficiency of double-strand break-dependent stress-induced mutation in Escherichia coli? J Mol Microb Biotech 21, 8-19, doi: 10.1159/000335354 (2011).
    • (2011) J Mol Microb Biotech , vol.21 , pp. 8-19
    • Shee, C.1    Ponder, R.2    Gibson, J.L.3    Rosenberg, S.M.4
  • 48
    • 80051961878 scopus 로고    scopus 로고
    • Impact of a stress-inducible switch to mutagenic repair of DNA breaks on mutation in Escherichia coli
    • Shee, C., Gibson, J. L., Darrow, M. C., Gonzalez, C. & Rosenberg, S. M. Impact of a stress-inducible switch to mutagenic repair of DNA breaks on mutation in Escherichia coli. Proc Natl Acad Sci USA 108, 13659-13664, doi: 10.1073/pnas.1104681108 (2011).
    • (2011) Proc Natl Acad Sci USA , vol.108 , pp. 13659-13664
    • Shee, C.1    Gibson, J.L.2    Darrow, M.C.3    Gonzalez, C.4    Rosenberg, S.M.5
  • 49
    • 84940466800 scopus 로고    scopus 로고
    • Development of a stress-induced mutagenesis module for autonomous adaptive evolution of Escherichia coli to improve its stress tolerance
    • ARTN93
    • Zhu, L. J., Li, Y. & Cai, Z. Development of a stress-induced mutagenesis module for autonomous adaptive evolution of Escherichia coli to improve its stress tolerance. Biotechnol Biofuels 8, doi: ARTN 9310.1186/s13068-015-0276-1 (2015).
    • (2015) Biotechnol Biofuels , vol.8
    • Zhu, L.J.1    Li, Y.2    Cai, Z.3
  • 50
    • 67349270900 scopus 로고    scopus 로고
    • Enzymatic assembly of DNA molecules up to several hundred kilobases
    • Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6, 343-345, doi: 10.1038/ Nmeth.1318 (2009).
    • (2009) Nat Methods , vol.6 , pp. 343-345
    • Gibson, D.G.1


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