Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded

Nature Communications

Volumn 8, Issue , 2017, Pages

  Wrenbeck, Emily E ^a   Azouz, Laura R ^a   Whitehead, Timothy A ^a,b  

^a Michigan State University   (United States)

^b Michigan State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALIPHATIC AMIDE HYDROLASE; AMIDASE; AMIDE; BACTERIAL ENZYME; ENZYME VARIANT; HYDROLASE; MESSENGER RNA; UNCLASSIFIED DRUG; ACETAMIDE; ACETAMIDE DERIVATIVE; ISOBUTYRAMIDE; PROPIONAMIDE;

BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; HYDROLYSIS; MOLECULAR ANALYSIS; MUTATION; PROTEIN; SUBSTRATE;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYTIC EFFICIENCY; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE MUTATION; IN VITRO STUDY; MISSENSE MUTATION; MOLECULAR EVOLUTION; NONHUMAN; NONSENSE MUTATION; POINT MUTATION; PROTEIN HYDROLYSIS; PSEUDOMONAS AERUGINOSA; SINGLE MUTATION FITNESS; BIOLOGICAL MODEL; CHEMISTRY; GENE LIBRARY; GENETIC SELECTION; GENETICS; GENOTYPE; HYDROLYSIS; MUTAGENESIS; MUTATION; PLASMID; REPRODUCTIVE FITNESS; SENSITIVITY AND SPECIFICITY;

ACETAMIDES; AMIDES; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; GENE LIBRARY; GENETIC FITNESS; GENOTYPE; HYDROLYSIS; MODELS, GENETIC; MUTAGENESIS; MUTATION; PLASMIDS; SELECTION, GENETIC; SENSITIVITY AND SPECIFICITY; SUBSTRATE SPECIFICITY;

EID: 85020295301     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms15695     Document Type: Article

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