Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases

Nature Communications

Volumn 7, Issue , 2016, Pages

  Van Der Meer, Jan Ytzen ^a   Poddar, Harshwardhan ^a   Baas, Bert Jan ^a,c   Miao, Yufeng ^a,d   Rahimi, Mehran ^a   Kunzendorf, Andreas ^a   Van Merkerk, Ronald ^a   Tepper, Pieter G ^a   Geertsema, Edzard M ^a,e   Thunnissen, Andy Mark W H ^b   Quax, Wim J ^a   Poelarends, Gerrit J ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

^c UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^d JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^e HANZE UNIVERSITY OF APPLIED SCIENCES   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

4 OXALOCROTONATE TAUTOMERASE; ALDEHYDE DERIVATIVE; ALKENE DERIVATIVE; ENZYME; UNCLASSIFIED DRUG; 4-OXALOCROTONATE TAUTOMERASE; CROTONIC ACID DERIVATIVE; ISOMERASE; OXALOCROTONATE;

ACETALDEHYDE; AMINO ACID; BIOENGINEERING; CATALYST; ENZYME ACTIVITY; GENE EXPRESSION;

ARTICLE; BIOCATALYST; ENANTIOSELECTIVITY; ENZYME ENGINEERING; ENZYME STRUCTURE; MICHAEL ADDITION; PROTEIN EXPRESSION; REACTION ANALYSIS; BIOCATALYSIS; CHEMISTRY; ENZYME SPECIFICITY; GENETICS; KINETICS; METABOLISM; PROTEIN ENGINEERING; STEREOISOMERISM;

BIOCATALYSIS; CROTONATES; ISOMERASES; KINETICS; PROTEIN ENGINEERING; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

EID: 84960428419     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms10911     Document Type: Article

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