Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects

Genome Biology

Volumn 18, Issue 1, 2017, Pages

  Gao, Yuanpeng ^a   Wu, Haibo ^a   Wang, Yongsheng ^a   Liu, Xin ^a   Chen, Linlin ^a   Li, Qian ^a   Cui, Chenchen ^a   Liu, Xu ^a   Zhang, Jingcheng ^a   Zhang, Yong ^a  

^a NORTHWEST A F UNIVERSITY   (China)

Author keywords

Chromatin immunoprecipitation sequencing (ChIP seq); CRISPR Cas9; Homologous recombination; Nickase; Off target; Single strand break; Tuberculosis

Indexed keywords

CAS9 NICKASE; CRISPR ASSOCIATED PROTEIN; NATURAL RESISTANCE ASSOCIATED MACROPHAGE PROTEIN 1; UNCLASSIFIED DRUG; CATION TRANSPORT PROTEIN; DEOXYRIBONUCLEASE I; GUIDE RNA; NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1; PROTEIN BINDING;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BOVINE TUBERCULOSIS; CELL NUCLEUS TRANSPLANTATION; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; CRISPR CAS SYSTEM; FEMALE; GENE INSERTION; GENE TARGETING; MALE; MYCOBACTERIUM BOVIS; NATURAL RESISTANCE ASSOCIATED MACROPHAGE PROTEIN 1 GENE; NONHUMAN; PLASMID; PREDICTION; TRANSGENE; TRANSGENIC CATTLE; ANIMAL; BINDING SITE; BOVINE; DISEASE RESISTANCE; GENE EXPRESSION REGULATION; GENE LOCUS; GENE ORDER; GENE VECTOR; GENETICS; METABOLISM; RECOMBINATION REPAIR; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BINDING SITES; CATION TRANSPORT PROTEINS; CATTLE; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASE I; DISEASE RESISTANCE; GENE KNOCK-IN TECHNIQUES; GENE ORDER; GENE TARGETING; GENETIC LOCI; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; NUCLEAR TRANSFER TECHNIQUES; PROTEIN BINDING; RECOMBINATIONAL DNA REPAIR; RNA, GUIDE; TRANSGENES;

EID: 85011279013     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/s13059-016-1144-4     Document Type: Article

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