Purification, characterization and bioactivity determination of a novel polysaccharide from pumpkin (Cucurbita moschata) seeds

Food Hydrocolloids

Volumn 66, Issue , 2017, Pages 357-364

  Wang, Libo ^a   Liu, Fangcheng ^a   Wang, Aoxue ^a   Yu, Zeyuan ^a   Xu, Yaqin ^a   Yang, Yu ^a  

^a NORTHEAST AGRICULTURAL UNIVERSITY   (China)

Author keywords

Antioxidant activity; Characterization; Polysaccharides; Pumpkin seeds; Purification

Indexed keywords

EID: 85008155500     PISSN: 0268005X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodhyd.2016.12.003     Document Type: Article

References (42)

1. Agrawal, P.K., NMR spectroscopy in the structure elucidation of oligosaccharides and glycosides. Phytochemistry 31 (1992), 3307–3330.
2. Cantu-Jungles, T.M., Maria-Ferreira, D., Silva, L.M., Baggio, C.H., Werner, M.F.P., Iacomini, M., et al. Polysaccharides from prunes: Gastroprotective activity and structural elucidation of bioactive pectins. Food Chemistry 146 (2014), 492–499.
3. Chen, Y., Xie, M.Y., Nie, S.P., Li, C., Wang, Y.X., Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chemistry 107 (2008), 231–241.
4. Chi, Y., Wang, L.B., Study on phenol-sulfuric acid method for determination of polysaccharide content in pumpkin seeds. Food and Machinery 30 (2014), 89–92.
5. Diao, W.R., Hu, Q.P., Zhang, H., Xu, J.G., Chemical composition, antibacterial activity and mechanism of action of essential oil from seeds of fennel (Foeniculum vulgare Mill.). Food Control 35 (2014), 109–116.
6. Du, B.J., Song, Y., Hu, X.S., Liao, X.J., Ni, Y.Y., et al. Oligosaccharides prepared by acid hydrolysis of polysaccharides from pumpkin (Cucurbita moschata) pulp and their prebiotic activities. International Journal of Food Science and Technology 46 (2011), 982–987.
7. Fu, C.L., Shi, H., Li, Q.H., A review on pharmacological activities and utilization technologies of pumpkin. Plant Foods for Human Nutrition 61 (2006), 73–80.
8. Fu, C.L., Tian, H.J., Cai, T.Y., Liu, Y., Li, Q.H., Some properties of an acidic protein-bound polysaccharide from the fruit of pumpkin. Food Chemistry 100 (2007), 944–947.
9. Gao, J., Zhang, T., Jin, Z.Y., Xu, X.M., Wang, J.H., Zha, X.Q., Structural characterisation, physicochemical properties and antioxidant activity of polysaccharide from Lilium lancifolium Thunb. Food Chemistry 169 (2015), 430–438.
10. He, L., Ji, P.F., Cheng, J.W., Wang, Y.B., Qian, H., Li, W.Q., et al. Structural characterization and immunostimulatory activity of a novel protein-bound polysaccharide produced by Hirsutella sinensis Liu, Guo, Yu & Zeng. Food Chemistry 141 (2013), 946–953.
11. Iwo, M.I., Insanu, M., Dass, C.A.S., Development of immunonutrient from pumpkin (Cucurbita moschata Duchense Ex. Lamk.) Seed. Procedia Chemistry 13 (2014), 105–111.
12. Jiao, J., Li, Z.G., Gai, Q.Y., Li, X.J., Wei, F.Y., Fu, Y.J., et al. Microwave-assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties, fatty acid compositions and antioxidant activities. Food Chemistry 147 (2014), 17–24.
13. Jun, H.I., Lee, C.H., Song, G.S., Kim, Y.S., Characterization of the pectic polysaccharides from pumpkin peel. LWT-Food Science and Technology 39 (2006), 554–561.
14. Kostálová, Z., Hromádková, Z., Ebringerová, A., Polovka, M., Michaelsen, T.E., Paulsen, B.S., Polysaccharides from the Styrian oil-pumpkin with antioxidant and complement-fixing activity. Industrial Crops and Products 41 (2013), 127–133.
15. Kozarski, M., Klaus, A., Jakovljevic, D., Todorovic, N., Niksic, M., Vrvic, M.M., et al. Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: Chemical characterization and bioactivities at different levels of purification. Food Research International 64 (2014), 53–64.
16. Li, Q.H., Fu, C.L., Rui, Y.K., Hu, G.H., Cai, T.Y., Effects of protein-bound polysaccharide isolated from pumpkin on insulin in diabetic rats. Plant Foods for Human Nutrition 60 (2005), 13–16.
17. Li, S.Q., Shah, N.P., Antioxidant and antibacterial activities of sulphated polysaccharides from Pleurotus eryngii and Streptococcus thermophilus ASCC 1275. Food Chemistry 165 (2014), 262–270.
18. Lott, J.A., Stephan, V.A., Pritchard, K.A., Evaluation of the Coomassie brilliant blue G-250 method for urinary protein. Clinical Chemistry 29 (1983), 1946–1950.
19. Matthewsa, K.K., O'Briena, D.J., Whitley, N.C., Burkec, J.M., Millerd, J.E., Barczewski, R.A., Investigation of possible pumpkin seeds and ginger effects ongastrointestinal nematode infection indicators in meat goat kids and lambs. Small Ruminant Research 136 (2016), 1–6.
20. Nep, E.I., Conway, B.R., Characterization of grewia gum, a potential pharmaceutical excipient. Journal of Excipients and Food Chemicals 1 (2010), 30–40.
21. Reziga, L., Chouaibi, M., Msaadab, K., Hamdi, S., Chemical composition and profile characterisation of pumpkin(Cucuibita maxima) seed oil. Industrial Crops and Products 37 (2012), 82–87.
22. Song, Y., Zhang, Y.Y., Zhou, T.T., Zhang, H., Hu, X.S., Li, Q.H., A preliminary study of monosaccharide compositionand α-glucosidase inhibitory effect of polysaccharides from pumpkin (Cucurbita moschata) fruit. International Journal of Food Science and Technology 47 (2012), 357–361.
23. Suvakanta, D., Narsimha, M.P., Pulak, D., Joshabir, C., Biswajit, D., Optimization and characterization of purified polysaccharide from Musa sapientum L. as a pharmaceutical excipient. Food Chemistry 149 (2014), 76–83.
24. Szterk, A., Roszko, M., Sosinska, E., Derewiaka, D., Lewicki, P.P., Chemical composition and oxidative stability of selected plant oils. Journal of the American Oil Chemists Society 87 (2010), 637–645.
25. Tan, H.F., Gan, C.Y., Polysaccharide with antioxidant, α-amylase inhibitory and ACE inhibitory activities from Momordica charantia. International Journal of Biological Macromolecules 85 (2016), 487–496.
26. Wang, L.B., Cheng, L., Xu, Y.Q., Wang, T.H., Li, L.B., Wang, H.S., Extraction technology optimization and antioxidant activity of polysaccharides from pumpkin seeds. Transactions of the Chinese Society of Agricultural Engineering 32 (2016), 284–290.
27. Wang, Y., Lu, Z.X., Wu, H., Lv, F.X., Study on the antibiotic activity of microcapsule curcumin against food borne pathogens. International Journal of Food Microbiology 136 (2009), 71–74.
28. Wang, F., Wang, W., Huang, Y., Liu, Z., Zhang, J., Characterization of a novel polysaccharide purified from a herb of Cynomorium songaricum Rupr. Food Hydrocolloids 47 (2015), 79–86.
29. Wang, L.B., Xu, Y.Q., Yang, Y., Sun, X., Aqueous enzymatic extraction of pumpkin seed oil and its physical-chemical properties. Transactions of the Chinese Society of Agricultural Engineering 27 (2011), 383–387.
30. Wang, L.B., Xu, Y.Q., Yu, Z.Y., Wang, T.H., Li, L.B., Ethanol fractional precipitation and ultrasonic modification of pumpkin polysaccharides. Transaction of the Chinese Society for Agricultural Machinery 46 (2015), 215–220.
31. Wang, Y., Yin, H.P., Lv, X.B., Wang, Y.F., Gao, H.Y., Wang, M., Protection of chronic renal failure by a polysaccharide from Cordyceps sinensis. Fitoerapia 81 (2010), 397–402.
32. Xanthopoulou, M.N., Nomikos, T., Fragopoulou, E., Antonopoulou, S., Antioxidant and lipoxygenase inhibitory activities of pumpkin seed extracts. Food Research International 42 (2009), 641–646.
33. Xie, J.H., Xie, M.Y., Nie, S.P., Shen, M.Y., Wang, Y.X., Li, C., Isolation, chemical composition and antioxidant activities of a water-soluble polysaccharide from Cyclocarya paliurus (Batal.) Iljinskaja. Food Chemistry 2010:119 (2010), 1626–1632.
34. Xu, Y.Q., Zhang, L., Yang, Y., Song, X.M., Yu, Z.Y., Optimization of ultrasound-assisted compound enzymatic extractionand characterization of polysaccharides from blackcurrant. Carbohydrate Polymers 117 (2015), 895–902.
35. 4-caused liver damage in mice. Food Chemistry 138 (2013), 1256–1264.
36. Yang, X.B., Zhao, Y., Lv, Y., Chemical composition and antioxidant activity of an acidic polysaccharide extracted from Cucurbita moschata Duchesne ex Poiret. Journal of Agricultural and Food Chemistry 55 (2007), 4684–4690.
37. Yu, Z.Y., Liu, L., Xu, Y.Q., Wang, L.B., Teng, X., Li, X.G., et al. Characterization and biological activities of a novel polysaccharide isolated from raspberry (Rubus idaeus L.) fruits. Carbohydrate Polymers 132 (2015), 180–186.
38. Zhang, J., Cui, J.H., Yin, T.T., Sun, L.Z., Li, G.X., Activated effect of lignin on α-amylase. Food Chemistry 141 (2013), 2229–2237.
39. Zhang, G.F., Su, D.Y., Zhang, Y.J., Jin, H., Chen, P., Zhu, L.Y., et al. The hypoglycemic effect of the pumpkin polysaccharide to the different diabetic model mice. Journal of Chinese Institute of Food Science and Technology 14 (2014), 23–27.
40. Zhao, X.H., Qian, L., Yin, D.L., Zhou, Y., Hypolipidemic effect of the polysaccharides extracted from pumpkin by cellulase-assisted method on mice. International Journal of Biological Macromolecules 64 (2014), 137–138.
41. Zhao, H., Wang, Q.H., Sun, Y.P., Yang, B.Y., Wang, Z.B., Chai, G.F., et al. Purification, characterization and immunomodulatory effects of Plantago depressa polysaccharides. Carbohydrate Polymers 112 (2014), 63–72.
42. Zou, C., Du, Y., Li, Y., Yang, J., Zhang, L., Kennedy, J.F., Preparation of lacquer polysaccharide sulfates and their antioxidant activity in vitro. Carbohydrate Polymers 73 (2008), 322–323.