Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB-co-4HB) biosynthesis

Metabolic Engineering

Volumn 29, Issue , 2015, Pages 160-168

  Lv, Li ^a   Ren, Yi Lin ^a   Chen, Jin Chun ^a   Wu, Qiong ^a   Chen, Guo Qiang ^a,b  

^a Tsinghua University   (China)

^b TSINGHUA UNIVERSITY   (China)

Author keywords

4 hydroxybutyrate; CRISPRi; Metabolic engineering; P3HB4HB; PHB; Synthetic biology

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; GENES; METABOLIC ENGINEERING; METABOLISM;

4-HYDROXYBUTYRATE; CRISPRI; P3HB4HB; PHB; SYNTHETIC BIOLOGY;

GENE EXPRESSION REGULATION;

4 HYDROXYBUTYRIC ACID; BUTYRIC ACID DERIVATIVE; CARBON; GLUCOSE; GUIDE RNA; POLY(3 HYDROXYBUTYRATE CO 4 HYDROXYBUTYRATE); POLYHYDROXYALKANOIC ACID; SUCCINATE DEHYDROGENASE; SUCCINATE SEMIALDEHYDE DEHYDROGENASE; SUCCINYL COENZYME A SYNTHETASE; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN; HYDROXYBUTYRIC ACID; POLY(3-HYDROXYBUTYRATE-CO-4-HYDROXYBUTYRATE); POLYESTER;

ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; DOWN REGULATION; ESCHERICHIA COLI; ESSENTIAL GENE; FEASIBILITY STUDY; GENE EXPRESSION REGULATION; METABOLIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; SDHA GENE; SDHB GENE; SUCC GENE; SUCD GENE; CRISPR CAS SYSTEM; ENZYMOLOGY; GENETICS; METABOLISM;

ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

CRISPR-CAS SYSTEMS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROXYBUTYRATES; METABOLIC ENGINEERING; POLYESTERS;

EID: 84926645319     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.03.013     Document Type: Article

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