New technology and resources for cryptococcal research

Fungal Genetics and Biology

Volumn 78, Issue , 2015, Pages 99-107

  Zhang, Nannan ^a   Park, Yoon Dong ^a   Williamson, Peter R ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Cryptococcosis; Cryptococcus gattii; Cryptococcus neoformans; Pathogenic fungus; RNAi; Yeast

Indexed keywords

FUNGAL PROTEIN; GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN; SHORT HAIRPIN RNA;

ARTICLE; CODON; CRYPTOCOCCOSIS; CRYPTOCOCCUS NEOFORMANS; FILOBASIDIELLA; FUNGAL GENOME; FUNGAL VIRULENCE; GENE DELETION; GENE DISRUPTION; GENE SEQUENCE; GENETIC ANALYSIS; HOMOLOGOUS RECOMBINATION; HOST PATHOGEN INTERACTION; MICROARRAY ANALYSIS; MICROSCOPY; MOLECULAR BIOLOGY; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEOMICS; RESEARCH; RNA INTERFERENCE; RNA SEQUENCE; SIGNAL TRANSDUCTION; GENE EXPRESSION PROFILING; GENE SILENCING; GENE TARGETING; GENETICS; GENOMICS; MEDICAL RESEARCH; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PATHOGENICITY; PROCEDURES; TRENDS;

CRYPTOCOCCUS GATTII; FILOBASIDIELLA NEOFORMANS; FUNGI;

BIOMEDICAL RESEARCH; CRYPTOCOCCOSIS; CRYPTOCOCCUS; GENE EXPRESSION PROFILING; GENE SILENCING; GENE TARGETING; GENOMICS; HOST-PATHOGEN INTERACTIONS; MICROBIOLOGICAL TECHNIQUES; MOLECULAR BIOLOGY; PROTEOMICS;

EID: 84988273383     PISSN: 10871845     EISSN: 10960937     Source Type: Journal    
DOI: 10.1016/j.fgb.2014.11.001     Document Type: Article

References (133)

1. Adler A., Park Y.D., Larsen P., Nagarajan V., Wollenberg K., Qiu J., Myers T.G., Williamson P.R. A novel specificity protein 1 (SP1)-like gene regulating protein kinase C-1 (Pkc1)-dependent cell wall integrity and virulence factors in Cryptococcus neoformans. J. Biol. Chem. 2011, 286:20977-20990.
2. Alvarez M., Casadevall A. Phagosome extrusion and host-cell survival after Cryptococcus neoformans phagocytosis by macrophages. Curr. Biol. 2006, 16:2161-2165.
3. Apidianakis Y., Rahme L.G., Heitman J., Ausubel F.M., Calderwood S.B., Mylonakis E. Challenge of Drosophila melanogaster with Cryptococcus neoformans and role of the innate immune response. Eukaryot. Cell 2004, 3:413-419.
4. Baker L.G., Lodge J.K. Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system. Meth. Mol. Biol. 2012, 845:85-98.
5. Baker L.G., Specht C.A., Donlin M.J., Lodge J.K. Chitosan, the deacetylated form of chitin, is necessary for cell wall integrity in Cryptococcus neoformans. Eukaryot. Cell 2007, 6:855-867.
6. Banerjee U., Datta K., Majumdar T., Gupta K. Cryptococcosis in India: the awakening of a giant?. Med. Mycol. 2001, 39:51-67.
7. Belay T., Cherniak R., Kozel T.R., Casadevall A. Reactivity patterns and epitope specificities of anti-Cryptococcus neoformans monoclonal antibodies by enzyme-linked immunosorbent assay and dot enzyme assay. Infect. Immun. 1997, 65:718-728.
8. Bose I., Doering T.L. Efficient implementation of RNA interference in the pathogenic yeast Cryptococcus neoformans. J. Microbiol. Meth. 2011, 86:156-159.
9. Botts M.R., Giles S.S., Gates M.A., Kozel T.R., Hull C.M. Isolation and characterization of Cryptococcus neoformans spores reveal a critical role for capsule biosynthesis genes in spore biogenesis. Eukaryot. Cell 2009, 8:595-605.
10. Calo S., Shertz-Wall C., Lee S.C., Bastidas R.J., Nicolas F.E., Granek J.A., Mieczkowski P., Torres-Martinez S., Ruiz-Vazquez R.M., Cardenas M.E., et al. Antifungal drug resistance evoked via RNAi-dependent epimutations. Nature 2014, 513:555-558.
11. Casadevall A. Amoeba provide insight into the origin of virulence in pathogenic fungi. Adv. Exp. Med. Biol. 2012, 710:1-10.
12. Casadevall A., DeShaw M., Fan M., Dromer F., Kozel T.R., Pirofski L.A. Molecular and idiotypic analysis of antibodies to Cryptococcus neoformans glucuronoxylomannan. Infect. Immun. 1994, 62:3864-3872.
13. Celotto A.M., Lee J.W., Graveley B.R. Exon-specific RNA interference. a tool to determine the functional relevance of proteins encoded by alternatively spliced mRNAs. Meth. Mol. Biol. 2005, 309:273-282.
14. Chang Y.C., Kwon-Chung K.J. Complementation of a capsule-deficient mutation of Cryptococcus neoformans restores its virulence. Mol. Cell. Biol. 1994, 14:4912-4919.
15. Chaturvedi V., Wong B., Newman S. Oxidative killing of Cryptococcus neoformans by human neutrophils. Evidence that fungal mannitol protects by scavenging reactive oxygen intermediates. J. Immunol. 1996, 156:3836-3840.
16. Chen Y., Toffaletti D.L., Tenor J.L., Litvintseva A.P., Fang C., Mitchell T.G., McDonald T.R., Nielsen K., Boulware D.R., Bicanic T., et al. The Cryptococcus neoformans transcriptome at the site of human meningitis. mBio 2014, 5:e01087-01013.
17. Chrisman C.J., Albuquerque P., Guimaraes A.J., Nieves E., Casadevall A. Phospholipids trigger Cryptococcus neoformans capsular enlargement during interactions with amoebae and macrophages. PLoS Pathogens 2011, 7:e1002047.
18. Chun C.D., Brown J.C., Madhani H.D. A major role for capsule-independent phagocytosis-inhibitory mechanisms in mammalian infection by Cryptococcus neoformans. Cell Host Microbe 2011, 9:243-251.
19. Cleare W., Casadevall A. Scanning electron microscopy of encapsulated and non-encapsulated Cryptococcus neoformans and the effect of glucose on capsular polysaccharide release. Med. Mycol. 1999, 37:235-243.
20. Clemons K.V., Azzi R., Stevens D.A. Experimental systemic cryptococcosis in SCID mice. J. Med. Vet. Mycol. 1996, 34:331-335.
21. Cox G.M., Rude T.H., Dykstra C.C., Perfect J.R. The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis. J. Med. Vet. Mycol. 1995, 33:261-266.
22. Cox G.M., Toffaletti D.L., Perfect J.R. Dominant selection system for use in Cryptococcus neoformans. J. Med. Vet. Mycol. 1996, 34:385-391.
23. Davidson R.C., Cruz M.C., Sia R.A., Allen B., Alspaugh J.A., Heitman J. Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans. Fungal Genet. Biol. 2000, 29:38-48.
24. Davidson R.C., Blankenship J.R., Kraus P.R., de Jesus Berrios M., Hull C.M., D'Souza C., Wang P., Heitman J. A PCR-based strategy to generate integrative targeting alleles with large regions of homology. Microbiology 2002, 148:2607-2615.
25. Derengowski Lda S., Paes H.C., Albuquerque P., Tavares A.H., Fernandes L., Silva-Pereira I., Casadevall A. The transcriptional response of Cryptococcus neoformans to ingestion by Acanthamoeba castellanii and macrophages provides insights into the evolutionary adaptation to the mammalian host. Eukaryot. Cell 2013, 12:761-774.
26. Ding C., Festa R.A., Chen Y.L., Espart A., Palacios O., Espin J., Capdevila M., Atrian S., Heitman J., Thiele D.J. Cryptococcus neoformans copper detoxification machinery is critical for fungal virulence. Cell Host Microbe 2013, 13:265-276.
27. D'Souza C.A., Kronstad J.W., Taylor G., Warren R., Yuen M., Hu G., Jung W.H., Sham A., Kidd S.E., Tangen K., et al. Genome variation in Cryptococcus gattii, an emerging pathogen of immunocompetent hosts. mBio 2011, 2:e00342-00310.
28. Edman J.C. Isolation of telomere like sequences from Cryptococcus neoformans and their use in high-efficiency transformation. Mol. Cell. Biol. 1992, 12:2777-2783.
29. Edman J.C., Kwon-Chung K.J. Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation. Mol. Cell. Biol. 1990, 10:4538-4544.
30. Edwards M.R., Gordon M.A., Lapa E.W., Ghiorse W.C. Micromorphology of Cryptococcus neoformans. J. Bacteriol. 1967, 94:766-777.
31. Erickson T., Liu L., Gueyikian A., Zhu X., Gibbons J., Williamson P. Multiple virulence factors of Cryptococcus neoformans are dependent on VPH1. Mol. Microbiol. 2001, 42:1121-1131.
32. Erickson T., Liu L., Gueyikian A., Zhu X., Gibbons J., Williamson P.R. Multiple virulence factors of Cryptococcus neoformans are dependent on VPH1. Mol. Microbiol. 2001, 42:1121-1131.
33. Falschlehner C., Steinbrink S., Erdmann G., Boutros M. High-throughput RNAi screening to dissect cellular pathways: a how-to guide. Biotechnol. J. 2010, 5:368-376.
34. Fan W., Kraus P., Boily M., Heitman J. Cryptococcus neoformans gene expression during murine macrophage infection. Eukaryot. Cell 2005, 4:1420-1433.
35. Feldmesser M., Casadevall A., Kress Y., Spira G., Orlofsky A. Eosinophil-Cryptococcus neoformans interactions in vivo and in vitro. Infect. Immun. 1997, 65:1899-1907.
36. Feldmesser M., Rivera J., Kress Y., Kozel T.R., Casadevall A. Antibody interactions with the capsule of Cryptococcus neoformans. Infect. Immun. 2000, 68:3642-3650.
37. Feldmesser M., Kress Y., Casadevall A. Dynamic changes in the morphology of Cryptococcus neoformans during murine pulmonary infection. Microbiology 2001, 147:2355-2365.
38. Florio A.R., Ferrari S., De Carolis E., Torelli R., Fadda G., Sanguinetti M., Sanglard D., Posteraro B. Genome-wide expression profiling of the response to short-term exposure to fluconazole in Cryptococcus neoformans serotype A. BMC Microbiol. 2011, 11:97.
39. Fonseca F.L., Guimaraes A.J., Kmetzsch L., Dutra F.F., Silva F.D., Taborda C.P., Araujo Gde S., Frases S., Staats C.C., Bozza M.T., et al. Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis. Fungal Genet. Biol. 2013, 60:64-73.
40. Fox D.S., Cox G.M., Heitman J. Phospholipid-binding protein Cts1 controls septation and functions coordinately with calcineurin in Cryptococcus neoformans. Eukaryot. Cell 2003, 2:1025-1035.
41. Fraser J.A., Subaran R.L., Nichols C.B., Heitman J. Recapitulation of the sexual cycle of the primary fungal pathogen Cryptococcus neoformans var. gattii: implications for an outbreak on Vancouver Island, Canada. Eukaryot. Cell 2003, 2:1036-1045.
42. Fraser J.A., Giles S.S., Wenink E.C., Geunes-Boyer S.G., Wright J.R., Diezmann S., Allen A., Stajich J.E., Dietrich F.S., Perfect J.R., et al. Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak. Nature 2005, 437:1360-1364.
43. Fu J., Hettler E., Wickes B.L. Split marker transformation increases homologous integration frequency in Cryptococcus neoformans. Fungal Genet. Biol. 2006, 43:200-212.
44. Giles S.S., Dagenais T.R., Botts M.R., Keller N.P., Hull C.M. Elucidating the pathogenesis of spores from the human fungal pathogen Cryptococcus neoformans. Infect. Immun. 2009, 77:3491-3500.
45. Goins C.L., Gerik K.J., Lodge J.K. Improvements to gene deletion in the fungal pathogen Cryptococcus neoformans: absence of Ku proteins increases homologous recombination, and co-transformation of independent DNA molecules allows rapid complementation of deletion phenotypes. Fungal Genet. Biol. 2006, 43:531-544.
46. Gorlach J.M., McDade H.C., Perfect J.R., Cox G.M. Antisense repression in Cryptococcus neoformans as a laboratory tool and potential antifungal strategy. Microbiology 2002, 148:213-219.
47. Goulart L., Rosa e Silva L.K., Chiapello L., Silveira C., Crestani J., Masih D., Vainstein M.H. Cryptococcus neoformans and Cryptococcus gattii genes preferentially expressed during rat macrophage infection. Med. Mycol. 2010, 48:932-941.
48. Haynes B.C., Skowyra M.L., Spencer S.J., Gish S.R., Williams M., Held E.P., Brent M.R., Doering T.L. Toward an integrated model of capsule regulation in Cryptococcus neoformans. PLoS Pathogens 2011, 7:e1002411.
49. He X., Lyons D.M., Toffaletti D.L., Wang F., Qiu Y., Davis M.J., Meister D.L., Dayrit J.K., Lee A., Osterholzer J.J., et al. Virulence factors identified by Cryptococcus neoformans mutant screen differentially modulate lung immune responses and brain dissemination. Am. J. Pathol. 2012, 181:1356-1366.
50. Hensel M., Shea J.E., Gleeson C., Jones M.D., Dalton E., Holden D.W. Simultaneous identification of bacterial virulence genes by negative selection. Science 1995, 269:400-403.
51. Hu G., Kronstad J.W. Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition. Curr. Genet. 2006, 1-10.
52. Hu G., Hacham M., Waterman S.R., Panepinto J., Shin S., Liu X., Gibbons J., Valyi-Nagy T., Obara K., Jaffe H.A., et al. PI3K signaling of autophagy is required for starvation tolerance and virulence of Cryptococcus neoformans. J. Clin. Invest. 2008, 118:1186-1197.
53. Hu G., Chen S.H., Qiu J., Bennett J.E., Myers T.G., Williamson P.R. Microevolution during serial mouse passage demonstrates FRE3 as a virulence adaptation gene in Cryptococcus neoformans. mBio 2014, 5:e00941-00914.
54. Hua J., et al. Development of positive selectable markers for the fungal pathogen Cryptococcus neoformans. Clin. Diagn. Lab. Immunol. 2000, 7:125-128.
55. Idnurm A., Reedy J.L., Nussbaum J.C., Heitman J. Cryptococcus neoformans virulence gene discovery through insertional mutagenesis. Eukaryot. Cell 2004, 3:420-429.
56. Idnurm A., Bahn Y.S., Nielsen K., Lin X., Fraser J.A., Heitman J. Deciphering the model pathogenic fungus Cryptococcus neoformans. Nat. Rev. Microbiol. 2005, 3:753-764.
57. Idnurm A., Walton F.J., Floyd A., Reedy J.L., Heitman J. Identification of ENA1 as a virulence gene of the human pathogenic fungus Cryptococcus neoformans through signature-tagged insertional mutagenesis. Eukaryot. Cell 2009, 8:315-326.
58. Janbon G., Maeng S., Yang D.H., Ko Y.J., Jung K.W., Moyrand F., Floyd A., Heitman J., Bahn Y.S. Characterizing the role of RNA silencing components in Cryptococcus neoformans. Fungal Genet. Biol. 2010, 47:1070-1080.
59. Janbon G., Ormerod K.L., Paulet D., Byrnes E.J., Yadav V., Chatterjee G., Mullapudi N., Hon C.C., Billmyre R.B., Brunel F., et al. Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation. PLoS Genetics 2014, 10:e1004261.
60. Jiang N., Yang Y., Janbon G., Pan J., Zhu X. Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans. PLoS One 2012, 7:e52734.
61. Johnston S.A., May R.C. The human fungal pathogen Cryptococcus neoformans escapes macrophages by a phagosome emptying mechanism that is inhibited by Arp2/3 complex-mediated actin polymerisation. PLoS Pathogens 2010, 6:e1001041.
62. Kelly R.M., Chen J., Yauch L.E., Levitz S.M. Opsonic requirements for dendritic cell-mediated responses to Cryptococcus neoformans. Infect. Immun. 2005, 73:592-598.
63. Kim M.S., Kim S.Y., Yoon J.K., Lee Y.W., Bahn Y.S. An efficient gene-disruption method in Cryptococcus neoformans by double-joint PCR with NAT-split markers. Biochem. Biophys. Res. Commun. 2009, 390:983-988.
64. 2 sensing pathway via differential expression of carbonic anhydrase in Cryptococcus neoformans. Genetics 2010, 185:1207-1219.
65. Kirkpatrick W.R., Najvar L.K., Bocanegra R., Patterson T.F., Graybill J.R. New guinea pig model of Cryptococcal meningitis. Antimicrob. Agents Chemother. 2007, 51:3011-3013.
66. Kozubowski L., Thompson J.W., Cardenas M.E., Moseley M.A., Heitman J. Association of calcineurin with the COPI protein Sec28 and the COPII protein Sec13 revealed by quantitative proteomics. PLoS One 2011, 6:e25280.
67. Kozubowski L., Yadav V., Chatterjee G., Sridhar S., Yamaguchi M., Kawamoto S., Bose I., Heitman J., Sanyal K. Ordered kinetochore assembly in the human-pathogenic basidiomycetous yeast Cryptococcus neoformans. mBio 2013, 4:e00614-00613.
68. Kwon-Chung K.J., Varma A., Edman J.C., Bennett J.E. Selection of ura5 and ura3 mutants from the two varieties of Cryptococcus neoformans on 5-fluoroorotic acid medium. J. Med. Vet. Mycol. 1992, 30:61-69.
69. Kwon-Chung J., Boekhout T., Fell J.W., Diaz M. Proposal to conserve the name Cryptococcus gattii against C. hondurianus and C. bacillisporus (Basidomycota, Hymenomycetes, Tremellomycetidae). Taxon 2002, 51:804-806.
70. Lazcano O., Speights V.O., Bilbao J., Becker J., Diaz J. Combined Fontana-Masson-mucin staining of Cryptococcus neoformans. Arch. Pathol. Lab. Med. 1991, 115:1145-1149.
71. Lin X., Hull C.M., Heitman J. Sexual reproduction between partners of the same mating type in Cryptococcus neoformans. Nature 2005, 434:1017-1021.
72. Liu J., Balasubramanian M.K. 1,3-Beta-glucan synthase: a useful target for antifungal drugs. Curr. Drug Targets Infect. Disord. 2001, 1:159-169.
73. Liu T.B., Xue C. Fbp1-mediated ubiquitin-proteasome pathway controls Cryptococcus neoformans virulence by regulating fungal intracellular growth in macrophages. Infect. Immun. 2014, 82:557-568.
74. Liu H., Cottrell T., Pierini L., Goldman W., Doering T. RNA interference in the pathogenic fungus Cryptococcus neoformans. Genetics 2002, 160:463-470.
75. Liu X., Hu G., Panepinto J., Williamson P. Role of a VPS41 homolog in starvation response and virulence of Cryptococcus neoformans. Mol. Microbiol. 2006, 61:1132-1146.
76. Liu O.W., Chun C.D., Chow E.D., Chen C., Madhani H.D., Noble S.M. Systematic genetic analysis of virulence in the human fungal pathogen Cryptococcus neoformans. Cell 2008, 135:174-188.
77. Lodge J.K., Jackson-Machelski E., Toffaletti D.L., Perfect J.R., Gordon J.I. Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans. Proc. Natl. Acad. Sci. USA 1994, 91:12008-12012.
78. Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., et al. The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. Science 2005, 307:1321-1324.
79. Ma H., Croudace J.E., Lammas D.A., May R.C. Expulsion of live pathogenic yeast by macrophages. Curr. Biol. 2006, 16:2156-2160.
80. Mambula S.S., Simons E.R., Hastey R., Selsted M.E., Levitz S.M. Human neutrophil-mediated nonoxidative antifungal activity against Cryptococcus neoformans. Infect. Immun. 2000, 68:6257-6264.
81. Mansour M., Schlesinger L., Levitz S. Optimal T cell responses to Cryptococcus neoformans mannoprotein are dependent on recognition of conjugated carbohydrates by mannose receptors. J. Immunol. 2002, 168:2872-2879.
82. McClelland C.M., Chang Y.C., Kwon-Chung K.J. High frequency transformation of Cryptococcus neoformans and Cryptococcus gattii by Agrobacterium tumefaciens. Fungal Genet. Biol. 2005, 42:904-913.
83. McDade H.C., Cox G.M. A new dominant selectable marker for use in Cryptococcus neoformans. Med. Mycol. 2001, 39:151-154.
84. Monari C., Baldelli F., Pietrella D., Retini C., Tascini C., Francisci D., Bistoni F., Vecchiarelli A. Monocyte dysfunction in patients with acquired immunodeficiency syndrome (AIDS) versus Cryptococcus neoformans. J. Infect. 1997, 35:257-263.
85. Mondon P., Chang Y.C., Varma A., Kwon-Chung K.J. A novel episomal shuttle vector for transformation of Cryptococcus neoformans with the ccdB gene as a positive selection marker in bacteria. FEMS Microbiol. Lett. 2000, 187:41-45.
86. Monheit J.E., Cowan D.F., Moore D.G. Rapid detection of fungi in tissues using calcofluor white and fluorescence microscopy. Arch. Pathol. Lab. Med. 1984, 108:616-618.
87. Murphy J.W., Hidore M.R., Wong S.C. Direct interactions of human lymphocytes with the yeast-like organism, Cryptococcus neoformans. J. Clin. Invest. 1993, 91:1553-1566.
88. Mylonakis E., Ausubel F.M., Perfect J.R., Heitman J., Calderwood S.B. Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesis. Proc. Natl. Acad. Sci. USA 2002, 99:15675-15680.
89. Nakayashiki H., Kadotani N., Mayama S. Evolution and diversification of RNA silencing proteins in fungi. J. Mol. Evol. 2006, 63:127-135.
90. Narasipura S., Ault J., Behr M., Chaturvedi V., Chaturvedi S. Characterization of Cu, Zn superoxide dismutase (SOD1) gene knock-out mutant of Cryptococcus neoformans var. gattii: role in biology and virulence. Mol. Microbiol. 2003, 47:1681-1694.
91. Nelson R.T., Hua J., Pryor B., Lodge J.K. Identification of virulence mutants of the fungal pathogen Cryptococcus neoformans using signature-tagged mutagenesis. Genetics 2001, 157:935-947.
92. O'Meara T.R., Alspaugh J.A. The Cryptococcus neoformans capsule: a sword and a shield. Clin. Microbiol. Rev. 2012, 25:387-408.
93. O'Meara T.R., Norton D., Price M.S., Hay C., Clements M.F., Nichols C.B., Alspaugh J.A. Interaction of Cryptococcus neoformans Rim101 and protein kinase A regulates capsule. PLoS Pathogens 2010, 6:e1000776.
94. O'Meara T.R., Holmer S.M., Selvig K., Dietrich F., Alspaugh J.A. Cryptococcus neoformans Rim101 is associated with cell wall remodeling and evasion of the host immune responses. mBio 2013, 4:e00522-00512.
95. Ory J.J., Griffith C.L., Doering T.L. An efficiently regulated promoter system for Cryptococcus neoformans utilizing the CTR4 promoter. Yeast 2004, 21:919-926.
96. Panepinto J., Liu L., Ramos J., Zhu X., Valyi-Nagy T., Eksi S., Fu J., Jaffe H., Wickes B., Williamson P. The DEAD-box RNA helicase Vad1 regulates multiple virulence-associated genes in Cryptococcus neoformans. J. Clin. Invest. 2005, 115:632-641.
97. Panepinto J., Komperda K., Frases S., Park Y.D., Djordjevic J.T., Casadevall A., Williamson P.R. Sec6-dependent sorting of fungal extracellular exosomes and laccase of Cryptococcus neoformans. Mol. Microbiol. 2009, 71:1165-1176.
98. Park B.J., Wannemuehler K.A., Marston B.J., Govender N., Pappas P.G., Chiller T.M. Estimation of the current global burden of cryptococcal meningitis among persons living with HIV/AIDS. AIDS 2009, 23:525-530.
99. Park Y.D., Shin S., Panepinto J., Ramos J., Qiu J., Frases S., Albuquerque P., Cordero R.J., Zhang N., Himmelreich U., et al. A role for LHC1 in higher order structure and complement binding of the Cryptococcus neoformans capsule. PLoS Pathogens 2014, 10:e1004037.
100. Perfect J.R., Lang S.D., Durack D.T. Chronic cryptococcal meningitis: a new experimental model in rabbits. Am. J. Pathol. 1980, 101:177-194.
101. Prudencio M., Lehmann M.J. Illuminating the host - how RNAi screens shed light on host-pathogen interactions. Biotechnol. J. 2009, 4:826-837.
102. Qin Q.M., Luo J., Lin X., Pei J., Li L., Ficht T.A., de Figueiredo P. Functional analysis of host factors that mediate the intracellular lifestyle of Cryptococcus neoformans. PLoS Pathogens 2011, 7:e1002078.
103. Qiu J., Olszewski M.A., Williamson P.R. Cryptococcus neoformans growth and protection from innate immunity are dependent on expression of a virulence-associated DEAD-Box protein, Vad1. Infect. Immun. 2013, 81:777-788.
104. Reese A., Doering T. Cell wall alpha-1,3-glucan is required to anchor the Cryptococcus neoformans capsule. Mol. Microbiol. 2003, 50:1401-1409.
105. Rodrigues M.L., Alvarez M., Fonseca F.L., Casadevall A. Binding of the wheat germ lectin to Cryptococcus neoformans suggests an association of chitinlike structures with yeast budding and capsular glucuronoxylomannan. Eukaryot. Cell 2008, 7:602-609.
106. Rosen L.B., Freeman A.F., Yang L.M., Jutivorakool K., Olivier K.N., Angkasekwinai N., Suputtamongkol Y., Bennett J.E., Pyrgos V., Williamson P.R., et al. Anti-GM-CSF autoantibodies in patients with cryptococcal meningitis. J. Immunol. 2013, 190:3959-3966.
107. Ruff J.A., Lodge J.K., Baker L.G. Three galactose inducible promoters for use in C. neoformans var. grubii. Fungal Genet. Biol. 2009, 46:9-16.
108. Saenz H.L., Dehio C. Signature-tagged mutagenesis: technical advances in a negative selection method for virulence gene identification. Curr. Opin. Microbiol. 2005, 8:612-619.
109. Salas S.D., Bennett J.E., Kwon-Chung K.J., Perfect J.R., Williamson P.R. Effect of the laccase gene CNLAC1, on virulence of Cryptococcus neoformans. J. Exp. Med. 1996, 184:377-386.
110. Santi L., Beys-da-Silva W.O., Berger M., Calzolari D., Guimaraes J.A., Moresco J.J., Yates J.R. Proteomic profile of Cryptococcus neoformans biofilm reveals changes in metabolic processes. J. Proteome Res. 2014, 13:1545-1559.
111. Selvan L.D., Renuse S., Kaviyil J.E., Sharma J., Pinto S.M., Yelamanchi S.D., Puttamallesh V.N., Ravikumar R., Pandey A., Prasad T.S., et al. Phosphoproteome of Cryptococcus neoformans. J. Proteomics 2014, 97:287-295.
112. Srikanta D., Yang M., Williams M., Doering T.L. A sensitive high-throughput assay for evaluating host-pathogen interactions in Cryptococcus neoformans infection. PLoS One 2011, 6:e22773.
113. Steen B., Lian T., Zuyderduyn S., MacDonald W.K., Marra M., Jones S., Kronstad J.W. Temperature-regulated transcription in the pathogenic fungus Cryptococcus neoformans. Genome Res. 2002, 12:1386-1400.
114. Steen B.R., Zuyderduyn S., Toffaletti D.L., Marra M., Jones S.J., Perfect J.R., Kronstad J. Cryptococcus neoformans gene expression during experimental cryptococcal meningitis. Eukaryot. Cell 2003, 2:1336-1349.
115. Steenbergen J.N., Shuman H.A., Casadevall A. Cryptococcus neoformans interactions with amoebae suggest an explanation for its virulence and intracellular pathogenic strategy in macrophages. Proc. Natl. Acad. Sci. USA 2001, 98:15245-15250.
116. Swanson M.S., Hammer B.K. Legionella pneumophila pathogesesis: a fateful journey from amoebae to macrophages. Annu. Rev. Microbiol. 2000, 54:567-613.
117. Toffaletti D.L., Rude T.H., Johnston S.A., Durack D.T., Perfect J.R. Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA. J. Bacteriol. 1993, 175:1405-1411.
118. Tseng H.K., Liu C.P., Price M.S., Jong A.Y., Chang J.C., Toffaletti D.L., Betancourt-Quiroz M., Frazzitta A.E., Cho W.L., Perfect J.R. Identification of genes from the fungal pathogen Cryptococcus neoformans related to transmigration into the central nervous system. PLoS One 2012, 7:e45083.
119. Tucker S., Casadevall A. Replication of Cryptococcus neoformans in macrophages is accompanied by phagosomal permeabilization and accumulation of vesicles containing polysaccharide in the cytoplasm. Proc. Natl. Acad. Sci. USA 2002, 99:3165-3170.
120. Upadhya R., Campbell L.T., Donlin M.J., Aurora R., Lodge J.K. Global transcriptome profile of Cryptococcus neoformans during exposure to hydrogen peroxide induced oxidative stress. PLoS One 2013, 8:e55110.
121. Vallim M.A., Nichols C.B., Fernandes L., Cramer K.L., Alspaugh J.A. A Rac homolog functions downstream of Ras1 to control hyphal differentiation and high-temperature growth in the pathogenic fungus Cryptococcus neoformans. Eukaryot. Cell 2005, 4:1066-1078.
122. Varma A., Kwon-Chung K. Characterization of the glyceraldehyde-3-phosphate dehydrogenase gene [correction of glyceraldehyde-3-phosphate gene] and the use of its promoter for heterologous expression in Cryptococcus neoformans, a human pathogen. Gene 1999, 232:277.
123. Varma A., Wu S., Guo N., Liao W., Lu G., Li A., Hu Y., Bulmer G., Kwon-Chung K.J. Identification of a novel gene, URE2, that functionally complements a urease-negative clinical strain of Cryptococcus neoformans. Microbiology 2006, 152:3723-3731.
124. Velculescu V.E., Zhang L., Vogelstein B., Kinzler K.W. Serial analysis of gene expression. Science 1995, 270:484-487.
125. Verma S., Idnurm A. The Uve1 endonuclease is regulated by the white collar complex to protect Cryptococcus neoformans from UV damage. PLoS Genetics 2013, 9:e1003769.
126. Vu K., Tham R., Uhrig J.P., Thompson G.R., Na Pombejra S., Jamklang M., Bautos J.M., Gelli A. Invasion of the central nervous system by Cryptococcus neoformans requires a secreted fungal metalloprotease. mBio 2014, 5:e01101-01114.
127. Wang X., Hsueh Y.P., Li W., Floyd A., Skalsky R., Heitman J. Sex-induced silencing defends the genome of Cryptococcus neoformans via RNAi. Genes Develop. 2010, 24:2566-2582.
128. Waterman S.R., Park Y.D., Raja M., Qiu J., Hammoud D.A., O'Halloran T.V., Williamson P.R. Role of CTR4 in the virulence of Cryptococcus neoformans. mBio 2012, 3:e00285-00212.
129. Wickes B.L., Edman J.C. The Cryptococcus neoformans GAL7 gene and its use as an inducible promoter. Mol. Microbiol. 1995, 16:1099-1109.
130. Wolf J., Espadas-Moreno J., Luque-Garcia J.L., Casadevall A. Interaction of Cryptococcus neoformans extracellular vesicles with the cell wall. Eukaryot. Cell 2014.
131. Xue C., Bahn Y.S., Cox G.M., Heitman J. G protein-coupled receptor Gpr4 senses amino acids and activates the cAMP-PKA pathway in Cryptococcus neoformans. Mol. Biol. Cell. 2006, 17:667-679.
132. Zaragoza O., Alvarez M., Telzak A., Rivera J., Casadevall A. The relative susceptibility of mouse strains to pulmonary Cryptococcus neoformans infection is associated with pleiotropic differences in the immune response. Infect. Immun. 2007, 75:2729-2739.
133. Zhao W., Fanning M.L., Lane T. Efficient RNAi-based gene family knockdown via set cover optimization. Artif. Intell. Med. 2005, 35:61-73.