A PCR-based strategy to generate integrative targeting alleles with large regions of homology

Microbiology

Volumn 148, Issue 8, 2002, Pages 2607-2615

  Davidson, Robert C ^a   Blankenship, Jill R ^a   Kraus, Peter R ^a   de Jesus Berrios, Marisol ^a   Hull, Christina M ^a   D'Souza, Cletus ^a   Wang, Ping ^a   Heitman, Joseph ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Cryptococcus neoformans; Gene disruption; PCR overlap

Indexed keywords

ALLELE; ARTICLE; CONTROLLED STUDY; CRYPTOCOCCUS NEOFORMANS; DIPLOIDY; FUNGAL STRAIN; FUNGUS ISOLATION; GENE CONSTRUCT; GENE DELETION; GENE DISRUPTION; GENE SEQUENCE; GENE TARGETING; GENETIC ANALYSIS; GENOME; METHODOLOGY; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SEROTYPING; CHEMISTRY; CLASSIFICATION; GENETIC MARKER; GENETIC RECOMBINATION; GENETICS;

CRYPTOCOCCUS NEOFORMANS; FILOBASIDIELLA NEOFORMANS; FUNGI; MYXOGASTRIA;

FUNGAL DNA; FUNGAL PROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHOTRANSFERASE; SACCHAROMYCES CEREVISIAE PROTEIN; TOR1 PROTEIN, S CEREVISIAE;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ALLELES; CRYPTOCOCCUS NEOFORMANS; DNA, FUNGAL; FUNGAL PROTEINS; GENE TARGETING; GENETIC MARKERS; GENOME, FUNGAL; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE DELETION; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 0036667448     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-148-8-2607     Document Type: Article

References (47)

1. Cryptococcus neoformans mating and virulence are regulated by the G-protein α subunit GPA1 and cAMP
2. RAS1 regulates filamentation, mating and growth at high temperature of Cryptococcus neoformans
3. Morphogenesis of Cryptococcus neoformans
4. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae
5. Cryptococcus neoformans
6. Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformans
7. The Cryptococcus neoformans STE11 α gene is similar to other fungal mitogen- activated protein kinase kinase kinase (MAPKKK) genes but is mating type specific
8. Urease as a virulence factor in experimental cryptococcosis
9. Extracellular phospholipase activity is a virulence factor for Cryptococcus neoformans
10. Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans
11. Comparison of the roles of calcineurin in physiology and virulence in serotype D and serotype A strains of Cryptococcus neoformans
12. Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans
13. Strategy for deletion of complete open reading frames in Saccharomyces cerevisiae
14. Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation
15. Calcineurin regulatory subunit is essential for virulence and mediates interactions with FKBP12-FK506 in Cryptococcus neoformans
16. Pathogenesis of pulmonary Cryptococcus neoformans infection in the rat
17. Antisense repression in Cryptococcus neoformans as a laboratory tool and potential antifungal strategy
18. The Cryptococcus neoformans genome sequencing project
19. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
20. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension
21. Development of positive selectable markers for the fungal pathogen Cryptococcus neoformans
22. A new genus, Filobasidiella, the perfect state of Cryptococcus neoformans
23. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans
24. Cryptococcosis
25. Melaninlacking mutants of Cryptococcus neoformans and their virulence for mice
26. RNA interference in the pathogenic fungus Cryptococcus neoformans
27. Gene disruption with PCR products in Saccharomyces cerevisiae
28. A new dominant selectable marker for use in Cryptococcus neoformans
29. Cryptococcosis in the era of AIDS - 100 years after the discovery of Cryptococcus neoformans
30. The α-mating type locus of Cryptococcus neoformans contains a peptide pheromone gene
31. Identification of virulence mutants of the fungal pathogen Cryptococcus neoformans using signature-tagged mutagenesis
32. Sequence length required for homologous recombination in Cryptococcus neoformans
33. Calcineurin is required for virulence of Cryptococcus neoformans
34. Chronic cryptococcal meningitis: A new experimental model in rabbits
35. A putative cyclic peptide efflux pump encoded by the TOXA gene of the plant-pathogenic fungus Cochliobolus carbonum
36. Contributo alla morfologia e biologia dei blastomiceti che si sviluppano nei succhi di alcuni frutti
37. Getting started with yeast
38. Diploid strains of the pathogenic basidiomycete Cryptococcus neoformans are thermally dimorphic
39. Molecular analysis of the Cryptococcus neoformans ADE2 gene, a selectable marker for transformation and gene disruption
40. Biolistic DNA delivery for Cryptococcus neoformans transformation
41. Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA
42. Systematic genetic analysis with ordered arrays of yeast deletion mutants
43. PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae
44. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae
45. The G-protein β subunit GPB1 is required for mating and haploid fruiting in Cryptococcus neoformans
46. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions