Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans

Fungal Genetics and Biology

Volumn 29, Issue 1, 2000, Pages 38-48

  Davidson, Robert C ^a   Cruz, M Cristina ^a   Sia, Rey A L ^a   Allen, Brandy ^a   Alspaugh, J Andrew ^a   Heitman, Joseph ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Basidiomycete; Homologous recombination; Mycology; Pathogenic fungi

Indexed keywords

CALCINEURIN; FUNGAL DNA; GUANINE NUCLEOTIDE BINDING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE;

ARTICLE; BIOLISTIC TRANSFORMATION; CONTROLLED STUDY; CRYPTOCOCCUS NEOFORMANS; DNA RECOMBINATION; ELECTROPORATION; FUNGAL GENETICS; GENE DISRUPTION; NONHUMAN; PRIORITY JOURNAL; SEROTYPE; TECHNIQUE;

CRYPTOCOCCUS NEOFORMANS; FILOBASIDIELLA NEOFORMANS; FUNGI;

EID: 0034001825     PISSN: 10871845     EISSN: None     Source Type: Journal    
DOI: 10.1006/fgbi.1999.1180     Document Type: Article

References (35)

1. Alspaugh J. A., Perfect J. R., Heitman J. Cryptococcus neoformans mating and virulence are regulated by the G-protein α subunit GPA1 and cAMP. Genes Dev. 11:1997;3206-3217.
2. Casadevall A., Perfect J. R. Cryptococcus neoformans. Secondary Cryptococcus neoformans. 1998;ASM Press, Washington.
3. Chang Y. C., Kwon-Chung K. J. Complementation of a capsule-deficient mutation of Cryptococcus neoformans restores its virulence. Mol. Cell. Biol. 14:1994;4912-4919.
4. Chang Y. C., Kwon-Chung K. J. Isolation of the third capsule-associated gene,LE>CAP60, required for virulence in Cryptococcus neoformans. Infect. Immun. 66:1998;2230-2236.
5. Chang Y. C., Penoyer L. A., Kwon-Chung K. J. The second capsule gene of Cryptococcus neoformans,CAP64, is essential for virulence. Infect. Immun. 64:1996;1977-1983.
6. Cruz M. C., Cavallo L. M., Görlach J. M., Cox G., Perfect J. R., Cardenas M. E., Heitman J. Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans. Mol. Cell. Biol. 19:1999;4101-4112.
7. Cruz M. C., Sia R. A. L., Olson M., Cox G. M., Heitman J. Comparison of the roles of calcineurin in physiology and virulence in serotype D and serotype A strains of Cryptococcus neoformans. Infect. Immun. 68:2000;982-985.
8. Del Poeta M., Toffaletti D. L., Rude T. H., Dykstra C. C., Heitman J., Perfect J. R. Topoisomerase I is essential in Cryptococcus neoformans: Role in pathobiology and as an antifungal target. Genetics. 152:1999;167-178.
9. Edman, J. C., and Kwon-Chung, K. J.1990. Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation. Mol. Cell. Biol.10: 4538-4544.
10. Fasoli M. O., Kerridge D., Ryley J. F. Pathogenicity of 5-fluorocytosine resistant strains of Candida albicans. J. Med. Vet. Mycol. 28:1990;27-34.
11. Heitman J., Allen B., Alspaugh J. A., Kwon-Chung K. J. On the origins of congenic MATα and MATa strains of the pathogenic yeast Cryptococcus neoformans. Fung. Genet. Biol. 28:1999;1-5.
12. Ivanovics G., Marjai E., Dobozy A. The growth of purine mutants of Bacillus anthracis in the body of the mouse. J. Gen. Microbiol. 53:1968;147-162.
13. Kirsch D. R., Whitney R. R. Pathogenicity of Candida albicans auxotrophic mutants in experimental infections. Infect. Immun. 59:1991;3297-3300.
14. Kwon-Chung K. J. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia. 68:1976;942-946.
15. Kwon-Chung K. J., Bennett J. E. Distribution of α and a mating types of Cryptococcus neoformans among natural and clinical isolates. Am. J. Epidemiol. 108:1978;337-340.
16. Kwon-Chung K. J., Edman J. C., Wickes B. L. Genetic association of mating types and virulence in Cryptococcus neoformans. Infect. Immun. 60:1992;602-605.
17. Kwon-Chung K. J., Hill W. B. Studies on the pink adenine deficient strains of Candida albicans cultural and morphological characteriztics. Sabouraudia. 8:1970;48-49.
18. Lodge J. K., Jackson-Machelski E., Toffaletti D. L., Perfect J. R., Gordon J. I. Targeted gene replacement demonstrates that myristoyl-CoA:protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans. Proc. Natl. Acad. Sci. USA. 91:1994;12008-12012.
19. Manning M., Snoddy C. B., Fromtling R. A. Comparative pathogenicity of auxotrophic mutants of Candida albicans. Can. J. Microbiol. 30:1984;31-35.
20. McFarland W. C., Stocker B. A. Effect of different purine auxotrophic mutations on mouse-virulence of a Vi-positive strain of Salmonella dublin and of two strains of Salmonella typhimurium. Microb. Pathog. 3:1987;129-141.
21. Mitchell T. G., Perfect J. R. Cryptococcosis in the era of AIDS - 100 years after the discovery of Cryptococcus neoformans. Clin. Microbiol. Rev. 8:1995;515-548.
22. Moore T. D. E., Edman J. C. The α-mating type locus of Cryptococcus neoformans contains a peptide pheromone gene. Mol. Cell. Biol. 13:1993;1962-1970.
23. Odom A., Muir S., Lim E., Toffaletti D. L., Perfect J., Heitman J. Calcineurin is required for virulence of Cryptococcus neoformans. EMBO J. 16:1997;2576-2589.
24. Perfect J. R., Toffaletti D. L., Rude T. H. The gene encoding phosphoribosylaminoimidazole carboxylase (ADE2) is essential for growth of Cryptococcus neoformans in cerebrospinal fluid. Infect. Immun. 61:1993;4446-4451.
25. Pitkin J. W., Panaccione D. G., Walton J. D. A putative cyclic peptide efflux pump encoded by the TOXA gene of the plant-pathogenic fungus Cochliobolus carbonum. Microbiology. 142:1996;1557-1565.
26. Rayssiguier C., Thaler D. S., Radman M. The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature. 23:1989;396-401.
27. Rhodes J. C., Howard D. H. Isolation and characterization of arginine auxotrophs of Cryptococcus neoformans. Infect. Immun. 27:1980;910-914.
28. Salas S. D., Bennett J. E., Kwon-Chung K. J., Perfect J. R., Williamson P. R. Effect of the laccase gene, CNLAC1, on virulence of Cryptococcus neoformans. J. Exp. Med. 184:1996;377-386.
29. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
30. Shepherd M. G. Pathogenicity of morphological and auxotrophic mutants of Candida albicans in experimental infections. Infect. Immun. 50:1985;541-544.
31. Sherman F. Getting started with yeast. Guthrie C., Fink G. R. Methods in Enzymology. 1991;3-21 Academic Press, San Diego.
32. Sudarshan S., Davidson R. C., Heitman J., Alspaugh J. A. Molecular analysis of the Cryptococcus neoformans ADE2 gene, a selectable marker for transformation and gene disruption. Fungal Genet. Biol. 27:1999;36-48.
33. te Riele H., Maandag E. R., Berns A. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc. Natl. Acad. Sci. USA. 89:1992;5128-5132.
34. Toffaletti D. L., Perfect J. R. Biolistic DNA delivery for Cryptococcus neoformans transformation. Maresca B., Kobayashi G. S. Molecular Biology of Pathogenic Fungal: A Laboratory Manual. 1994;303-308 Telos Press, New York.
35. Toffaletti D. L., Rude T. H., Johnston S. A., Durack D. T., Perfect J. R. Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA. J. Bacteriol. 175:1993;1405-1411.