High frequency transformation of Cryptococcus neoformans and Cryptococcus gattii by Agrobacterium tumefaciens

Fungal Genetics and Biology

Volumn 42, Issue 11, 2005, Pages 904-913

  McClelland, Carol M ^a   Chang, Yun C ^a   Kwon Chung, K J ^b  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Agrobacterium tumefaciens; ATMT; C. gattii; Cryptococcus neoformans; Insertional mutagenesis; Transformation

Indexed keywords

ARTICLE; BIOTRANSFORMATION; CONTROLLED STUDY; CRYPTOCOCCUS; CRYPTOCOCCUS GATTII; CRYPTOCOCCUS NEOFORMANS; ELECTROPORATION; GENE FREQUENCY; GENE INSERTION; GENETIC TRANSFECTION; MOLECULAR MODEL; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RHIZOBIUM RADIOBACTER; SACCHAROMYCES CEREVISIAE; SEROTYPE;

CRYPTOCOCCUS; CRYPTOCOCCUS NEOFORMANS; PLASMIDS; RHIZOBIUM RADIOBACTER; TRANSFORMATION, BACTERIAL; TRANSFORMATION, GENETIC;

AGROBACTERIUM TUMEFACIENS; FILOBASIDIELLA NEOFORMANS; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 27544492512     PISSN: 10871845     EISSN: 10960937     Source Type: Journal    
DOI: 10.1016/j.fgb.2005.07.003     Document Type: Article

References (48)

1. C. Baron, N. Domke, M. Beinhofer, and S. Hapfelmeier Elevated temperature differentially affects virulence, VirB protein accumulation, and T-pilus formation in different Agrobacterium tumefaciens and Agrobacterium vitis strains J. Bacteriol. 183 2001 6852 6861
2. M. Bevan Binary Agrobacterium vectors for plant transformation Nucleic Acids Res. 12 1984 8711 8721
3. J.T. Bish, and A. Sarachek Influences of temperature and adenine concentration upon the cultural instability of a red adenine auxotroph of Candida albicans Mycologia 59 1967 671 688
4. M. Bolker, H.U. Bohnert, K.H. Braun, J. Gorl, and R. Kahmann Tagging pathogenicity genes in Ustilago maydis by restriction enzyme-mediated integration (REMI) Mol. Gen. Genet. 248 1995 547 552
5. J.S. Brown, A. Aufauvre-Brown, J. Brown, J.M. Jennings, H. Arst Jr., and D.W. Holden Signature-tagged and directed mutagenesis identify PABA synthetase as essential for Aspergillus fumigatus pathogenicity Mol. Microbiol. 36 2000 1371 1380
6. P. Bundock, A. den Dulk-Ras, A. Beijersbergen, and P.J. Hooykaas Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae EMBO J. 14 1995 3206 3214
7. Y.C. Chang, and K.J. Kwon-Chung Complementation of a capsule-deficient mutation of Cryptococcus neoformans restores its virulence Mol. Cell. Biol. 14 1994 4912 4919
8. Y.C. Chang, L.A. Penoyer, and K.J. Kwon-Chung The second capsule gene of Cryptococcus neoformans, CAP64, is essential for virulence Infect. Immun. 64 1996 1977 1983
9. Y.C. Chang, L.A. Penoyer, and K.J. Kwon-Chung The second STE12 homologue of Cryptococcus neoformans is MATa-specific and plays an important role in virulence Proc. Natl. Acad. Sci. USA 98 2001 3258 3263
10. Y.C. Chang, B.L. Wickes, and K.J. Kwon-Chung Further analysis of the CAP59 locus of Cryptococcus neoformans: structure defined by forced expression and description of a new ribosomal protein-encoding gene Gene 167 1995 179 183
11. J.P. Combier, D. Melayah, C. Raffier, G. Gay, and R. Marmeisse Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the symbiotic ectomycorrhizal fungus Hebeloma cylindrosporum FEMS Microbiol. Lett. 220 2003 141 148
12. R.C. Davidson, M.C. Cruz, R.A. Sia, B. Allen, J.A. Alspaugh, and J. Heitman Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans Fungal Genet. Biol. 29 2000 38 48
13. J.C. Edman, and K.J. Kwon-Chung Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation Mol. Cell. Biol. 10 1990 4538 4544
14. T. Erickson, L. Liu, A. Gueyikian, X. Zhu, J. Gibbons, and P.R. Williamson Multiple virulence factors of Cryptococcus neoformans are dependent on VPH1 Mol. Microbiol. 42 2001 1121 1131
15. J.A. Fraser, R.L. Subaran, C.B. Nichols, and J. Heitman Recapitulation of the sexual cycle of the primary fungal pathogen Cryptococcus neoformans var. gattii: implications for an outbreak on Vancouver Island, Canada Eukaryot. Cell 2 2003 1036 1045
16. K.J. Fullner, and E.W. Nester Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens J. Bacteriol. 178 1996 1498 1504
17. D.M. Gardiner, and B.J. Howlett Negative selection using thymidine kinase increases the efficiency of recovery of transformants with targeted genes in the filamentous fungus Leptosphaeria maculans Curr. Genet. 45 2004 249 255
18. C.L. Griffith, J.S. Klutts, L. Zhang, S.B. Levery, and T.L. Doering UDP-glucose dehydrogenase plays multiple roles in the biology of the pathogenic fungus Cryptococcus neoformans J. Biol. Chem. 279 2004 51669 51676
19. A. Idnurm, J.L. Reedy, J.C. Nussbaum, and J. Heitman Cryptococcus neoformans virulence gene discovery through insertional mutagenesis Eukaryot. Cell 3 2004 420 429
20. C.H. Khang, S.Y. Park, Y.H. Lee, and S. Kang A dual selection based, targeted gene replacement tool for Magnaporthe grisea and Fusarium oxysporum Fungal Genet. Biol. 42 2005 483 492
21. S.E. Kidd, F. Hagen, R.L. Tscharke, M. Huynh, K.H. Bartlett, M. Fyfe, L. Macdougall, T. Boekhout, K.J. Kwon-Chung, and W. Meyer A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada) Proc. Natl. Acad. Sci. USA 101 2004 17258 17263
22. C. Koncz, K. Nemeth, G.P. Redei, and J. Schell T-DNA insertional mutagenesis in Arabidopsis Plant. Mol. Biol. 20 1992 963 976
23. T. Kunik, T. Tzfira, Y. Kapulnik, Y. Gafni, C. Dingwall, and V. Citovsky Genetic transformation of HeLa cells by Agrobacterium Proc. Natl. Acad. Sci. USA 98 2001 1871 1876
24. K.J. Kwon-Chung, and J.E. Bennett Epidemiologic differences between the two varieties of Cryptococcus neoformans Am. J. Epidemiol. 120 1984 123 130
25. K.J. Kwon-Chung, A. Varma, J.C. Edman, and J.E. Bennett Selection of ura5 and ura3 mutants from the two varieties of Cryptococcus neoformans on 5-fluoroorotic acid medium J. Med. Vet. Mycol. 30 1992 61 69
26. H. Lee, Y.C. Chang, and K.J. Kwon-Chung TUP1 disruption reveals biological differences between MATa and MATa strains of cryptococcus neoformans Mol. Microbiol. 55 2005 1222 1232
27. F.J. Maier, and W. Schafer Mutagenesis via insertional- or restriction enzyme-mediated-integration (REMI) as a tool to tag pathogenicity related genes in plant pathogenic fungi Biol. Chem. 380 1999 855 864
28. H.C. McDade, and G.M. Cox A new dominant selectable marker for use in Cryptococcus neoformans Med. Mycol. 39 2001 151 154
29. C.B. Michielse, P.J. Hooykaas, C.A. van den Hondel, and A.F. Ram Agrobacterium-mediated transformation as a tool for functional genomics in fungi Curr. Genet. 2005
30. T.S. Mikosch, B. Lavrijssen, A.S. Sonnenberg, and L.J. van Griensven Transformation of the cultivated mushroom Agaricus bisporus (Lange) using T-DNA from Agrobacterium tumefaciens Curr. Genet. 39 2001 35 39
31. E.D. Mullins, and S. Kang Transformation: a tool for studying fungal pathogens of plants Cell. Mol. Life Sci. 58 2001 2043 2052
32. S.D. Narasipura, J.G. Ault, M.J. Behr, V. Chaturvedi, and S. Chaturvedi Characterization of Cu,Zn superoxide dismutase (SOD1) gene knock-out mutant of Cryptococcus neoformans var. gattii: role in biology and virulence Mol. Microbiol. 47 2003 1681 1694
33. R.T. Nelson, J. Hua, B. Pryor, and J.K. Lodge Identification of virulence mutants of the fungal pathogen Cryptococcus neoformans using signature-tagged mutagenesis Genetics 157 2001 935 947
34. P.R. Peachey, P.O. Gubbins, and R.E. Martin The association between cryptococcal variety and immunocompetent and immunocompromised hosts Pharmacotherapy 18 1998 255 264
35. J.R. Perfect, S.D.R. Lang, and D.T. Durack Chronic cryptococcal meningitis: A new experimental model in rabbits Am. J. Pathol. 101 1980 177 194
36. R.L. Roberts, M. Metz, D.E. Monks, M.L. Mullaney, T. Hall, and E.W. Nester Purine synthesis and increased Agrobacterium tumefaciens transformation of yeast and plants Proc. Natl. Acad. Sci. USA 100 2003 6634 6639
37. S. Rolland, C. Jobic, M. Fevre, and C. Bruel Agrobacterium-mediated transformation of Botrytis cinerea, simple purification of monokaryotic transformants and rapid conidia-based identification of the transfer-DNA host genomic DNA flanking sequences Curr. Genet. 44 2003 164 171
38. S.D. Salas, J.E. Bennett, K.J. Kwon-Chung, J.R. Perfect, and P.R. Williamson Effect of the laccase gene CNLAC1, on virulence of Cryptococcus neoformans J. Exp. Med. 184 1996 377 386
39. A. Sarachek Mutagenicity of cytosine for an adenine and arginine requiring strain of Candida albicans Mycopathol. Mycol. Appl. 26 1965 72 78
40. J.A. Sugui, Y.C. Chang, and K.J. Kwon-Chung Agrobacterium tumefaciens-mediated transformation of Aspergillus fumigatus: an efficient tool for insertional mutagenesis and targeted gene disruption Appl. Environ. Microbiol. 71 2005 1798 1802
41. T.D. Sullivan, P.J. Rooney, and B.S. Klein Agrobacterium tumefaciens integrates transfer DNA into single chromosomal sites of dimorphic fungi and yields homokaryotic progeny from multinucleate yeast Eukaryot. Cell 1 2002 895 905
42. D.L. Toffaletti, T.H. Rude, S.A. Johnston, D.T. Durack, and J.R. Perfect Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA J. Bacteriol. 175 1993 1405 1411
43. R.L. Tscharke, M. Lazera, Y.C. Chang, B.L. Wickes, and K.J. Kwon-Chung Haploid fruiting in Cryptococcus neoformans is not mating type α-specific Fungal Genet. Biol. 39 2003 230 237
44. M.A. Uhl, M. Biery, N. Craig, and A.D. Johnson Haploinsufficiency-based large-scale forward genetic analysis of filamentous growth in the diploid human fungal pathogen C. albicans EMBO J. 22 2003 2668 2678
45. A. Varma, J.C. Edman, and K.J. Kwon-Chung Molecular and genetic analysis of URA5 transformants of Cryptococcus neoformans Infec. Immun. 60 1992 1101 1108
46. A. Varma, and K.J. Kwon-Chung Characterization of the glyceraldehyde-3-phosphate dehydrogenase gene (correction of glyceraldehyde-3-phosphate gene) and the use of its promoter for heterologous expression in Cryptococcus neoformans, a human pathogen Gene 232 1999 155 163
47. M.J. Wagner, J.A. Sharp, and W.C. Summers Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1 Proc. Natl. Acad. Sci. USA 78 1981 1441 1445
48. J.P. Woods, D. Kersulyte, W.E. Goldman, and D.E. Berg Fast DNA isolation from Histoplasma capsulatum: methodology for arbitrary primer polymerase chain reaction-based epidemiological and clinical studies J. Clin. Microbiol. 31 1993 463 464