메뉴 건너뛰기




Volumn 42, Issue , 2016, Pages 178-188

Systems metabolic engineering of Escherichia coli for the heterologous production of high value molecules — a veteran at new shores

Author keywords

[No Author keywords available]

Indexed keywords

CELL ENGINEERING; ESCHERICHIA COLI; METABOLISM; MOLECULES; PRODUCTION PLATFORMS;

EID: 84982836684     PISSN: 09581669     EISSN: 18790429     Source Type: Journal    
DOI: 10.1016/j.copbio.2016.05.004     Document Type: Review
Times cited : (40)

References (83)
  • 1
    • 1542423138 scopus 로고
    • Production of L-threonine by auxotrophic mutants of Escherichia coli
    • 1 Huang, H.T., Production of L-threonine by auxotrophic mutants of Escherichia coli. Appl Microbiol 9 (1961), 419–424.
    • (1961) Appl Microbiol , vol.9 , pp. 419-424
    • Huang, H.T.1
  • 2
    • 0019800517 scopus 로고
    • Bacterial production of human insulin
    • 2 Riggs, A.D., Bacterial production of human insulin. Diabetes Care 4 (1981), 64–68.
    • (1981) Diabetes Care , vol.4 , pp. 64-68
    • Riggs, A.D.1
  • 3
    • 84923860609 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli for production of mixed-acid fermentation end products
    • 3 Förster, A.H., Gescher, J., Metabolic engineering of Escherichia coli for production of mixed-acid fermentation end products. Front Bioeng Biotechnol, 2, 2014, 16.
    • (2014) Front Bioeng Biotechnol , vol.2 , pp. 16
    • Förster, A.H.1    Gescher, J.2
  • 4
    • 84870172887 scopus 로고    scopus 로고
    • The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli
    • 4 Richter, K., Gescher, J., The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli. Biochem Soc Trans 40 (2012), 1222–1226.
    • (2012) Biochem Soc Trans , vol.40 , pp. 1222-1226
    • Richter, K.1    Gescher, J.2
  • 5
    • 84923868543 scopus 로고    scopus 로고
    • Advanced biotechnology metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products
    • 5 Becker, J., Wittmann, C., Advanced biotechnology metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products. Angew Chem Int Ed Engl 54 (2015), 3328–3350.
    • (2015) Angew Chem Int Ed Engl , vol.54 , pp. 3328-3350
    • Becker, J.1    Wittmann, C.2
  • 6
    • 84882921508 scopus 로고    scopus 로고
    • Systems metabolic engineering of Escherichia coli for chemicals, materials, biofuels, and pharmaceuticals
    • C Wittmann S.Y. Lee Springer
    • 6 Na, D., Park, J.H., Jang, Y.S., Lee, J.W., Lee, S.Y., Systems metabolic engineering of Escherichia coli for chemicals, materials, biofuels, and pharmaceuticals. Wittmann, C, Lee, S.Y., (eds.) Systems Metabolic Engineering, 2012, Springer, 117–149.
    • (2012) Systems Metabolic Engineering , pp. 117-149
    • Na, D.1    Park, J.H.2    Jang, Y.S.3    Lee, J.W.4    Lee, S.Y.5
  • 7
    • 84950277538 scopus 로고    scopus 로고
    • Microbial production of value-added nutraceuticals
    • 7 Wang, J., Guleria, S., Koffas, M.A., Yan, Y., Microbial production of value-added nutraceuticals. Curr Opin Biotechnol 37 (2015), 97–104.
    • (2015) Curr Opin Biotechnol , vol.37 , pp. 97-104
    • Wang, J.1    Guleria, S.2    Koffas, M.A.3    Yan, Y.4
  • 8
    • 84940206768 scopus 로고    scopus 로고
    • Advancing metabolic engineering through systems biology of industrial microorganisms
    • 8 Dai, Z., Nielsen, J., Advancing metabolic engineering through systems biology of industrial microorganisms. Curr Opin Biotechnol 36 (2015), 8–15.
    • (2015) Curr Opin Biotechnol , vol.36 , pp. 8-15
    • Dai, Z.1    Nielsen, J.2
  • 9
    • 84938415635 scopus 로고    scopus 로고
    • Microbial pigments as natural color sources: current trends and future perspectives
    • 9 Tuli, H.S., Chaudhary, P., Beniwal, V., Sharma, A.K., Microbial pigments as natural color sources: current trends and future perspectives. J Food Sci Technol 52 (2015), 4669–4678.
    • (2015) J Food Sci Technol , vol.52 , pp. 4669-4678
    • Tuli, H.S.1    Chaudhary, P.2    Beniwal, V.3    Sharma, A.K.4
  • 10
    • 84923923224 scopus 로고    scopus 로고
    • Systems metabolic engineering of Escherichia coli for gram scale production of the antitumor drug deoxyviolacein from glycerol
    • This paper reports on the heterologous production of the high-value therapeutic agent deoxyviolacein with an outstanding production titer of 1.6 g/L. The heart of the study is a tailor-made E. coli strain, which was engineered on a systems-wide level. Optimized process operations and streamlined purification yielded >99.5% pure deoxyviolacein.
    • 10•• Rodrigues, A.L., Becker, J., de Souza Lima, A.O., Porto, L.M., Wittmann, C., Systems metabolic engineering of Escherichia coli for gram scale production of the antitumor drug deoxyviolacein from glycerol. Biotechnol Bioeng 111 (2014), 2280–2289 This paper reports on the heterologous production of the high-value therapeutic agent deoxyviolacein with an outstanding production titer of 1.6 g/L. The heart of the study is a tailor-made E. coli strain, which was engineered on a systems-wide level. Optimized process operations and streamlined purification yielded >99.5% pure deoxyviolacein.
    • (2014) Biotechnol Bioeng , vol.111 , pp. 2280-2289
    • Rodrigues, A.L.1    Becker, J.2    de Souza Lima, A.O.3    Porto, L.M.4    Wittmann, C.5
  • 11
    • 84858009322 scopus 로고    scopus 로고
    • Microbial production of the drugs violacein and deoxyviolacein: analytical development and strain comparison
    • 11 Rodrigues, A.L., Göcke, Y., Bolten, C., Brock, N.L., Dickschat, J.S., Wittmann, C., Microbial production of the drugs violacein and deoxyviolacein: analytical development and strain comparison. Biotechnol Lett 34 (2012), 717–720.
    • (2012) Biotechnol Lett , vol.34 , pp. 717-720
    • Rodrigues, A.L.1    Göcke, Y.2    Bolten, C.3    Brock, N.L.4    Dickschat, J.S.5    Wittmann, C.6
  • 13
    • 84924140841 scopus 로고    scopus 로고
    • High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway
    • 13 Fang, M.Y., Zhang, C., Yang, S., Cui, J.Y., Jiang, P.X., Lou, K., Wachi, M., Xing, X.H., High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Microb Cell Fact, 14, 2015, 8.
    • (2015) Microb Cell Fact , vol.14 , pp. 8
    • Fang, M.Y.1    Zhang, C.2    Yang, S.3    Cui, J.Y.4    Jiang, P.X.5    Lou, K.6    Wachi, M.7    Xing, X.H.8
  • 16
    • 0034091478 scopus 로고    scopus 로고
    • Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production
    • 16 Stahmann, K.P., Revuelta, J.L., Seulberger, H., Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production. Appl Microbiol Biotechnol 53 (2000), 509–516.
    • (2000) Appl Microbiol Biotechnol , vol.53 , pp. 509-516
    • Stahmann, K.P.1    Revuelta, J.L.2    Seulberger, H.3
  • 17
    • 84858005266 scopus 로고    scopus 로고
    • Riboflavin production by Ashbya gossypii
    • 17 Kato, T., Park, E.Y., Riboflavin production by Ashbya gossypii. Biotechnol Lett 34 (2012), 611–618.
    • (2012) Biotechnol Lett , vol.34 , pp. 611-618
    • Kato, T.1    Park, E.Y.2
  • 18
    • 84923925548 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli for the production of riboflavin
    • 18 Lin, Z., Xu, Z., Li, Y., Wang, Z., Chen, T., Zhao, X., Metabolic engineering of Escherichia coli for the production of riboflavin. Microb Cell Fact, 13, 2014, 104.
    • (2014) Microb Cell Fact , vol.13 , pp. 104
    • Lin, Z.1    Xu, Z.2    Li, Y.3    Wang, Z.4    Chen, T.5    Zhao, X.6
  • 19
    • 35348981360 scopus 로고    scopus 로고
    • Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum — over expression and modification of G6P dehydrogenase
    • 19 Becker, J., Klopprogge, C., Herold, A., Zelder, O., Bolten, C.J., Wittmann, C., Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum — over expression and modification of G6P dehydrogenase. J Biotechnol 132 (2007), 99–109.
    • (2007) J Biotechnol , vol.132 , pp. 99-109
    • Becker, J.1    Klopprogge, C.2    Herold, A.3    Zelder, O.4    Bolten, C.J.5    Wittmann, C.6
  • 20
    • 11144261828 scopus 로고    scopus 로고
    • A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum
    • 20 Ohnishi, J., Katahira, R., Mitsuhashi, S., Kakita, S., Ikeda, M., A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum. FEMS Microbiol Lett 242 (2005), 265–274.
    • (2005) FEMS Microbiol Lett , vol.242 , pp. 265-274
    • Ohnishi, J.1    Katahira, R.2    Mitsuhashi, S.3    Kakita, S.4    Ikeda, M.5
  • 22
    • 84924488449 scopus 로고    scopus 로고
    • Production of salidroside in metabolically engineered Escherichia coli
    • 22 Bai, Y., Bi, H., Zhuang, Y., Liu, C., Cai, T., Liu, X., Zhang, X., Liu, T., Ma, Y., Production of salidroside in metabolically engineered Escherichia coli. Sci Rep, 4, 2014, 6640.
    • (2014) Sci Rep , vol.4 , pp. 6640
    • Bai, Y.1    Bi, H.2    Zhuang, Y.3    Liu, C.4    Cai, T.5    Liu, X.6    Zhang, X.7    Liu, T.8    Ma, Y.9
  • 23
    • 84958969892 scopus 로고    scopus 로고
    • Engineering the shikimate pathway for biosynthesis of molecules with pharmaceutical activities in E. coli
    • 23 Jiang, M., Zhang, H., Engineering the shikimate pathway for biosynthesis of molecules with pharmaceutical activities in E. coli. Curr Opin Biotechnol 42 (2016), 1–6.
    • (2016) Curr Opin Biotechnol , vol.42 , pp. 1-6
    • Jiang, M.1    Zhang, H.2
  • 25
    • 84922572075 scopus 로고    scopus 로고
    • Evaluation of the anti-inflammatory, anti-catabolic and pro-anabolic effects of E-caryophyllene, myrcene and limonene in a cell model of osteoarthritis
    • 25 Rufino, A.T., Ribeiro, M., Sousa, C., Judas, F., Salgueiro, L., Cavaleiro, C., Mendes, A.F., Evaluation of the anti-inflammatory, anti-catabolic and pro-anabolic effects of E-caryophyllene, myrcene and limonene in a cell model of osteoarthritis. Eur J Pharmacol 750 (2015), 141–150.
    • (2015) Eur J Pharmacol , vol.750 , pp. 141-150
    • Rufino, A.T.1    Ribeiro, M.2    Sousa, C.3    Judas, F.4    Salgueiro, L.5    Cavaleiro, C.6    Mendes, A.F.7
  • 26
    • 84929376563 scopus 로고    scopus 로고
    • Microbial synthesis of myrcene by metabolically engineered Escherichia coli
    • 26 Kim, E.M., Eom, J.H., Um, Y., Kim, Y., Woo, H.M., Microbial synthesis of myrcene by metabolically engineered Escherichia coli. J Agric Food Chem 63 (2015), 4606–4612.
    • (2015) J Agric Food Chem , vol.63 , pp. 4606-4612
    • Kim, E.M.1    Eom, J.H.2    Um, Y.3    Kim, Y.4    Woo, H.M.5
  • 27
    • 84899051891 scopus 로고    scopus 로고
    • Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development
    • 27 Paddon, C.J., Keasling, J.D., Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development. Nat Rev Microbiol 12 (2014), 355–367.
    • (2014) Nat Rev Microbiol , vol.12 , pp. 355-367
    • Paddon, C.J.1    Keasling, J.D.2
  • 28
    • 0038391517 scopus 로고    scopus 로고
    • Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
    • 28 Martin, V.J., Pitera, D.J., Withers, S.T., Newman, J.D., Keasling, J.D., Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol 21 (2003), 796–802.
    • (2003) Nat Biotechnol , vol.21 , pp. 796-802
    • Martin, V.J.1    Pitera, D.J.2    Withers, S.T.3    Newman, J.D.4    Keasling, J.D.5
  • 29
    • 84879142653 scopus 로고    scopus 로고
    • High-level production of amorpha-4,11-diene, a precursor of the antimalarial agent artemisinin, in Escherichia coli
    • This study is the first report on the production of the artemisinin precursor amorpha-4,11-diene at commercially relevant titers. Production performance of the recombinant strains was substantially optimized by selecting the most suitable enzyme variants for S. cerevisiae and S. aureus. Combined with a superior fermentation process, 27.4 g/L amorpha-4,11-diene was produced in fed-batch fermentation.
    • 29•• Tsuruta, H., Paddon, C.J., Eng, D., Lenihan, J.R., Horning, T., Anthony, L.C., Regentin, R., Keasling, J.D., Renninger, N.S., Newman, J.D., High-level production of amorpha-4,11-diene, a precursor of the antimalarial agent artemisinin, in Escherichia coli. PLoS One, 4, 2009, e4489 This study is the first report on the production of the artemisinin precursor amorpha-4,11-diene at commercially relevant titers. Production performance of the recombinant strains was substantially optimized by selecting the most suitable enzyme variants for S. cerevisiae and S. aureus. Combined with a superior fermentation process, 27.4 g/L amorpha-4,11-diene was produced in fed-batch fermentation.
    • (2009) PLoS One , vol.4 , pp. e4489
    • Tsuruta, H.1    Paddon, C.J.2    Eng, D.3    Lenihan, J.R.4    Horning, T.5    Anthony, L.C.6    Regentin, R.7    Keasling, J.D.8    Renninger, N.S.9    Newman, J.D.10
  • 30
    • 84938209211 scopus 로고    scopus 로고
    • Experimental design-aided systematic pathway optimization of glucose uptake and deoxyxylulose phosphate pathway for improved amorphadiene production
    • 30 Zhang, C., Zou, R., Chen, X., Stephanopoulos, G., Too, H.P., Experimental design-aided systematic pathway optimization of glucose uptake and deoxyxylulose phosphate pathway for improved amorphadiene production. Appl Microbiol Biotechnol 99 (2015), 3825–3837.
    • (2015) Appl Microbiol Biotechnol , vol.99 , pp. 3825-3837
    • Zhang, C.1    Zou, R.2    Chen, X.3    Stephanopoulos, G.4    Too, H.P.5
  • 32
    • 84951014894 scopus 로고    scopus 로고
    • Developing fermentative terpenoid production for commercial usage
    • 32 Leavell, M.D., McPhee, D.J., Paddon, C.J., Developing fermentative terpenoid production for commercial usage. Curr Opin Biotechnol 37 (2015), 114–119.
    • (2015) Curr Opin Biotechnol , vol.37 , pp. 114-119
    • Leavell, M.D.1    McPhee, D.J.2    Paddon, C.J.3
  • 33
    • 84955249209 scopus 로고    scopus 로고
    • Combinatorial engineering of hybrid mevalonate pathways in Escherichia coli for protoilludene production
    • 33 Yang, L., Wang, C., Zhou, J., Kim, S.W., Combinatorial engineering of hybrid mevalonate pathways in Escherichia coli for protoilludene production. Microb Cell Fact, 15, 2016, 14.
    • (2016) Microb Cell Fact , vol.15 , pp. 14
    • Yang, L.1    Wang, C.2    Zhou, J.3    Kim, S.W.4
  • 34
    • 84920077579 scopus 로고    scopus 로고
    • Natural and engineered production of taxadiene with taxadiene synthase
    • 34 Soliman, S., Tang, Y., Natural and engineered production of taxadiene with taxadiene synthase. Biotechnol Bioeng 112 (2015), 229–235.
    • (2015) Biotechnol Bioeng , vol.112 , pp. 229-235
    • Soliman, S.1    Tang, Y.2
  • 35
    • 84949575568 scopus 로고    scopus 로고
    • Synthetic biology for pharmaceutical drug discovery
    • 35 Trosset, J.Y., Carbonell, P., Synthetic biology for pharmaceutical drug discovery. Drug Des Devel Ther 9 (2015), 6285–6302.
    • (2015) Drug Des Devel Ther , vol.9 , pp. 6285-6302
    • Trosset, J.Y.1    Carbonell, P.2
  • 36
  • 39
    • 0034838359 scopus 로고    scopus 로고
    • Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol
    • 39 Huang, Q., Roessner, C.A., Croteau, R., Scott, A.I., Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol. Bioorg Med Chem 9 (2001), 2237–2242.
    • (2001) Bioorg Med Chem , vol.9 , pp. 2237-2242
    • Huang, Q.1    Roessner, C.A.2    Croteau, R.3    Scott, A.I.4
  • 40
    • 84926646130 scopus 로고    scopus 로고
    • Distributing a metabolic pathway among a microbial consortium enhances production of natural products
    • This study proposes a promising concept to link the synthetic capacity of different cells for production.
    • 40• Zhou, K., Qiao, K., Edgar, S., Stephanopoulos, G., Distributing a metabolic pathway among a microbial consortium enhances production of natural products. Nat Biotechnol 33 (2015), 377–383 This study proposes a promising concept to link the synthetic capacity of different cells for production.
    • (2015) Nat Biotechnol , vol.33 , pp. 377-383
    • Zhou, K.1    Qiao, K.2    Edgar, S.3    Stephanopoulos, G.4
  • 41
    • 18844392599 scopus 로고    scopus 로고
    • Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli
    • 41 Alper, H., Jin, Y.S., Moxley, J.F., Stephanopoulos, G., Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli. Metab Eng 7 (2005), 155–164.
    • (2005) Metab Eng , vol.7 , pp. 155-164
    • Alper, H.1    Jin, Y.S.2    Moxley, J.F.3    Stephanopoulos, G.4
  • 42
    • 22844452835 scopus 로고    scopus 로고
    • Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets
    • 42 Alper, H., Miyaoku, K., Stephanopoulos, G., Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets. Nat Biotechnol 23 (2005), 612–616.
    • (2005) Nat Biotechnol , vol.23 , pp. 612-616
    • Alper, H.1    Miyaoku, K.2    Stephanopoulos, G.3
  • 43
    • 84875670791 scopus 로고    scopus 로고
    • Engineering central metabolic modules of Escherichia coli for improving beta-carotene production
    • Authors applied a multi-module combinatory approach to optimize beta-carotene production in E. coli. Variations in the expression levels of selected genes from the TCA cycle, PP pathway, ATP synthesis, isoprenoid pathway, and terminal biosynthesis allowed the production of 2.1 g/L beta-carotene.
    • 43• Zhao, J., Li, Q., Sun, T., Zhu, X., Xu, H., Tang, J., Zhang, X., Ma, Y., Engineering central metabolic modules of Escherichia coli for improving beta-carotene production. Metab Eng 17 (2013), 42–50 Authors applied a multi-module combinatory approach to optimize beta-carotene production in E. coli. Variations in the expression levels of selected genes from the TCA cycle, PP pathway, ATP synthesis, isoprenoid pathway, and terminal biosynthesis allowed the production of 2.1 g/L beta-carotene.
    • (2013) Metab Eng , vol.17 , pp. 42-50
    • Zhao, J.1    Li, Q.2    Sun, T.3    Zhu, X.4    Xu, H.5    Tang, J.6    Zhang, X.7    Ma, Y.8
  • 44
    • 79955103635 scopus 로고    scopus 로고
    • Engineering of a plasmid-free Escherichia coli strain for improved in vivo biosynthesis of astaxanthin
    • 44 Lemuth, K., Steuer, K., Albermann, C., Engineering of a plasmid-free Escherichia coli strain for improved in vivo biosynthesis of astaxanthin. Microb Cell Fact, 10, 2011, 29.
    • (2011) Microb Cell Fact , vol.10 , pp. 29
    • Lemuth, K.1    Steuer, K.2    Albermann, C.3
  • 45
    • 84925511388 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli to produce zeaxanthin
    • 45 Li, X.R., Tian, G.Q., Shen, H.J., Liu, J.Z., Metabolic engineering of Escherichia coli to produce zeaxanthin. J Ind Microbiol Biotechnol 42 (2015), 627–636.
    • (2015) J Ind Microbiol Biotechnol , vol.42 , pp. 627-636
    • Li, X.R.1    Tian, G.Q.2    Shen, H.J.3    Liu, J.Z.4
  • 46
    • 84884591440 scopus 로고    scopus 로고
    • Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines
    • 46 Weichert, S., Jennewein, S., Hufner, E., Weiss, C., Borkowski, J., Putze, J., Schroten, H., Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines. Nutr Res 33 (2013), 831–838.
    • (2013) Nutr Res , vol.33 , pp. 831-838
    • Weichert, S.1    Jennewein, S.2    Hufner, E.3    Weiss, C.4    Borkowski, J.5    Putze, J.6    Schroten, H.7
  • 47
    • 85047696209 scopus 로고    scopus 로고
    • Enhanced production of 2′-fucosyllactose in engineered Escherichia coli BL21star(DE3) by modulation of lactose metabolism and fucosyltransferase
    • 47 Chin, Y.W., Kim, J.Y., Lee, W.H., Seo, J.H., Enhanced production of 2′-fucosyllactose in engineered Escherichia coli BL21star(DE3) by modulation of lactose metabolism and fucosyltransferase. J Biotechnol 210 (2015), 107–115.
    • (2015) J Biotechnol , vol.210 , pp. 107-115
    • Chin, Y.W.1    Kim, J.Y.2    Lee, W.H.3    Seo, J.H.4
  • 48
    • 84876816081 scopus 로고    scopus 로고
    • Construction of Escherichia coli strains with chromosomally integrated expression cassettes for the synthesis of 2′-fucosyllactose
    • The authors constructed the first selection of a marker-free E. coli strain that produces 2′-fucosyllactose from lactose and glycerol. Implementation of the full biosynthetic pathway for GDP-L-fucose allowed de novo synthesis of the high-priced fucose component from glycerol. Overall, a final 2′-fucosyllactose concentration of >20 g/L was achieved.
    • 48• Baumgärtner, F., Seitz, L., Sprenger, G.A., Albermann, C., Construction of Escherichia coli strains with chromosomally integrated expression cassettes for the synthesis of 2′-fucosyllactose. Microb Cell Fact, 12, 2013, 40 The authors constructed the first selection of a marker-free E. coli strain that produces 2′-fucosyllactose from lactose and glycerol. Implementation of the full biosynthetic pathway for GDP-L-fucose allowed de novo synthesis of the high-priced fucose component from glycerol. Overall, a final 2′-fucosyllactose concentration of >20 g/L was achieved.
    • (2013) Microb Cell Fact , vol.12 , pp. 40
    • Baumgärtner, F.1    Seitz, L.2    Sprenger, G.A.3    Albermann, C.4
  • 49
    • 84862215539 scopus 로고    scopus 로고
    • Whole cell biosynthesis of a functional oligosaccharide, 2′-fucosyllactose, using engineered Escherichia coli
    • 49 Lee, W.H., Pathanibul, P., Quarterman, J., Jo, J.H., Han, N.S., Miller, M.J., Jin, Y.S., Seo, J.H., Whole cell biosynthesis of a functional oligosaccharide, 2′-fucosyllactose, using engineered Escherichia coli. Microb Cell Fact, 11, 2012, 48.
    • (2012) Microb Cell Fact , vol.11 , pp. 48
    • Lee, W.H.1    Pathanibul, P.2    Quarterman, J.3    Jo, J.H.4    Han, N.S.5    Miller, M.J.6    Jin, Y.S.7    Seo, J.H.8
  • 50
    • 85027929533 scopus 로고    scopus 로고
    • Synthesis of the human milk oligosaccharide lacto-N-tetraose in metabolically engineered, plasmid-free E. coli
    • 50 Baumgärtner, F., Conrad, J., Sprenger, G.A., Albermann, C., Synthesis of the human milk oligosaccharide lacto-N-tetraose in metabolically engineered, plasmid-free E. coli. Chembiochem 15 (2014), 1896–1900.
    • (2014) Chembiochem , vol.15 , pp. 1896-1900
    • Baumgärtner, F.1    Conrad, J.2    Sprenger, G.A.3    Albermann, C.4
  • 52
    • 84958191367 scopus 로고    scopus 로고
    • Novel alpha-L-fucosidases from a soil metagenome for production of fucosylated human milk oligosaccharides
    • 52 Lezyk, M., Jers, C., Kjaerulff, L., Gotfredsen, C.H., Mikkelsen, M.D., Mikkelsen, J.D., Novel alpha-L-fucosidases from a soil metagenome for production of fucosylated human milk oligosaccharides. PLOS ONE, 11, 2016, e0147438.
    • (2016) PLOS ONE , vol.11 , pp. e0147438
    • Lezyk, M.1    Jers, C.2    Kjaerulff, L.3    Gotfredsen, C.H.4    Mikkelsen, M.D.5    Mikkelsen, J.D.6
  • 53
    • 84901927684 scopus 로고    scopus 로고
    • Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work
    • 53 Pena, C., Castillo, T., Garcia, A., Millan, M., Segura, D., Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work. Microb Biotechnol 7 (2014), 278–293.
    • (2014) Microb Biotechnol , vol.7 , pp. 278-293
    • Pena, C.1    Castillo, T.2    Garcia, A.3    Millan, M.4    Segura, D.5
  • 54
    • 85014870763 scopus 로고    scopus 로고
    • Diamines for bio-based materials
    • C. Wittmann J.C. Liao Wiley-VCH [in press]
    • 54 Becker, J., Wittmann, C., Diamines for bio-based materials. Wittmann, C., Liao, J.C., (eds.) Industrial Biotechnology, 2016, Wiley-VCH [in press].
    • (2016) Industrial Biotechnology
    • Becker, J.1    Wittmann, C.2
  • 55
    • 84939207951 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli for the production of 1,3-diaminopropane, a three carbon diamine
    • 55 Chae, T.U., Kim, W.J., Choi, S., Park, S.J., Lee, S.Y., Metabolic engineering of Escherichia coli for the production of 1,3-diaminopropane, a three carbon diamine. Sci Rep, 5, 2015, 13040.
    • (2015) Sci Rep , vol.5 , pp. 13040
    • Chae, T.U.1    Kim, W.J.2    Choi, S.3    Park, S.J.4    Lee, S.Y.5
  • 56
    • 84941217806 scopus 로고    scopus 로고
    • Top value platform chemicals: bio-based production of organic acids
    • 56 Becker, J., Lange, A., Fabarius, J., Wittmann, C., Top value platform chemicals: bio-based production of organic acids. Curr Opin Biotechnol 36 (2015), 168–175.
    • (2015) Curr Opin Biotechnol , vol.36 , pp. 168-175
    • Becker, J.1    Lange, A.2    Fabarius, J.3    Wittmann, C.4
  • 57
    • 77956296603 scopus 로고    scopus 로고
    • Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber
    • In this study, E. coli was, for the first time, engineered to produce spider dragline silk, an extraordinarily strong and elastic protein fiber with broad application potential. Strain optimization, high cell density cultivation and subsequent product purification yielded a total of 1.2 g of protein.
    • 57•• Xia, X.X., Qian, Z.G., Ki, C.S., Park, Y.H., Kaplan, D.L., Lee, S.Y., Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber. Proc Natl Acad Sci U S A 107 (2010), 14059–14063 In this study, E. coli was, for the first time, engineered to produce spider dragline silk, an extraordinarily strong and elastic protein fiber with broad application potential. Strain optimization, high cell density cultivation and subsequent product purification yielded a total of 1.2 g of protein.
    • (2010) Proc Natl Acad Sci U S A , vol.107 , pp. 14059-14063
    • Xia, X.X.1    Qian, Z.G.2    Ki, C.S.3    Park, Y.H.4    Kaplan, D.L.5    Lee, S.Y.6
  • 58
    • 0025008260 scopus 로고
    • Structure of a protein superfiber: spider dragline silk
    • 58 Xu, M., Lewis, R.V., Structure of a protein superfiber: spider dragline silk. Proc Natl Acad Sci U S A 87 (1990), 7120–7124.
    • (1990) Proc Natl Acad Sci U S A , vol.87 , pp. 7120-7124
    • Xu, M.1    Lewis, R.V.2
  • 59
    • 15444368211 scopus 로고    scopus 로고
    • Characterization of the protein components of Nephila clavipes dragline silk
    • 59 Sponner, A., Schlott, B., Vollrath, F., Unger, E., Grosse, F., Weisshart, K., Characterization of the protein components of Nephila clavipes dragline silk. Biochemistry 44 (2005), 4727–4736.
    • (2005) Biochemistry , vol.44 , pp. 4727-4736
    • Sponner, A.1    Schlott, B.2    Vollrath, F.3    Unger, E.4    Grosse, F.5    Weisshart, K.6
  • 60
    • 84865600352 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli BL21 for biosynthesis of heparosan, a bioengineered heparin precursor
    • 60 Zhang, C., Liu, L., Teng, L., Chen, J., Liu, J., Li, J., Du, G., Metabolic engineering of Escherichia coli BL21 for biosynthesis of heparosan, a bioengineered heparin precursor. Metab Eng 14 (2012), 521–527.
    • (2012) Metab Eng , vol.14 , pp. 521-527
    • Zhang, C.1    Liu, L.2    Teng, L.3    Chen, J.4    Liu, J.5    Li, J.6    Du, G.7
  • 61
    • 84885900286 scopus 로고    scopus 로고
    • High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production
    • 61 Restaino, O.F., Bhaskar, U., Paul, P., Li, L., De Rosa, M., Dordick, J.S., Linhardt, R.J., High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production. Appl Microbiol Biotechnol 97 (2013), 3893–3900.
    • (2013) Appl Microbiol Biotechnol , vol.97 , pp. 3893-3900
    • Restaino, O.F.1    Bhaskar, U.2    Paul, P.3    Li, L.4    De Rosa, M.5    Dordick, J.S.6    Linhardt, R.J.7
  • 62
    • 84865300402 scopus 로고    scopus 로고
    • Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12
    • 62 Barreteau, H., Richard, E., Drouillard, S., Samain, E., Priem, B., Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12. Carbohydr Res 360 (2012), 19–24.
    • (2012) Carbohydr Res , vol.360 , pp. 19-24
    • Barreteau, H.1    Richard, E.2    Drouillard, S.3    Samain, E.4    Priem, B.5
  • 63
    • 55549129643 scopus 로고    scopus 로고
    • A high-throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors
    • 63 Yu, H., Tyo, K., Alper, H., Klein-Marcuschamer, D., Stephanopoulos, G., A high-throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors. Biotechnol Bioeng 101 (2008), 788–796.
    • (2008) Biotechnol Bioeng , vol.101 , pp. 788-796
    • Yu, H.1    Tyo, K.2    Alper, H.3    Klein-Marcuschamer, D.4    Stephanopoulos, G.5
  • 64
    • 37349103842 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli for biosynthesis of hyaluronic acid
    • 64 Yu, H., Stephanopoulos, G., Metabolic engineering of Escherichia coli for biosynthesis of hyaluronic acid. Metab Eng 10 (2008), 24–32.
    • (2008) Metab Eng , vol.10 , pp. 24-32
    • Yu, H.1    Stephanopoulos, G.2
  • 65
    • 77955559190 scopus 로고    scopus 로고
    • Production of chondroitin sulfate and chondroitin
    • 65 Schiraldi, C., Cimini, D., De Rosa, M., Production of chondroitin sulfate and chondroitin. Appl Microbiol Biotechnol 87 (2010), 1209–1220.
    • (2010) Appl Microbiol Biotechnol , vol.87 , pp. 1209-1220
    • Schiraldi, C.1    Cimini, D.2    De Rosa, M.3
  • 67
    • 78149468483 scopus 로고    scopus 로고
    • Improved fructosylated chondroitin production by kfoC overexpression in E. coli K4
    • 67 Cimini, D., De Rosa, M., Viggiani, A., Restaino, O.F., Carlino, E., Schiraldi, C., Improved fructosylated chondroitin production by kfoC overexpression in E. coli K4. J Biotechnol 150 (2010), 324–331.
    • (2010) J Biotechnol , vol.150 , pp. 324-331
    • Cimini, D.1    De Rosa, M.2    Viggiani, A.3    Restaino, O.F.4    Carlino, E.5    Schiraldi, C.6
  • 68
  • 69
    • 84914129027 scopus 로고    scopus 로고
    • Production of chondroitin in metabolically engineered E. coli
    • 69 He, W., Fu, L., Li, G., Andrew Jones, J., Linhardt, R.J., Koffas, M., Production of chondroitin in metabolically engineered E. coli. Metab Eng 27 (2015), 92–100.
    • (2015) Metab Eng , vol.27 , pp. 92-100
    • He, W.1    Fu, L.2    Li, G.3    Andrew Jones, J.4    Linhardt, R.J.5    Koffas, M.6
  • 70
    • 84867209510 scopus 로고    scopus 로고
    • Systems and synthetic metabolic engineering for amino acid production — the heartbeat of industrial strain development
    • 70 Becker, J., Wittmann, C., Systems and synthetic metabolic engineering for amino acid production — the heartbeat of industrial strain development. Curr Opin Biotechnol 23 (2012), 718–726.
    • (2012) Curr Opin Biotechnol , vol.23 , pp. 718-726
    • Becker, J.1    Wittmann, C.2
  • 71
    • 84864801619 scopus 로고    scopus 로고
    • Bio-based production of chemicals, materials and fuels — Corynebacterium glutamicum as versatile cell factory
    • 71 Becker, J., Wittmann, C., Bio-based production of chemicals, materials and fuels — Corynebacterium glutamicum as versatile cell factory. Curr Opin Biotechnol 23 (2012), 631–640.
    • (2012) Curr Opin Biotechnol , vol.23 , pp. 631-640
    • Becker, J.1    Wittmann, C.2
  • 72
    • 84938950996 scopus 로고    scopus 로고
    • Engineering microbial cell factories: metabolic engineering of Corynebacterium glutamicum with a focus on non-natural products
    • 72 Heider, S.A., Wendisch, V.F., Engineering microbial cell factories: metabolic engineering of Corynebacterium glutamicum with a focus on non-natural products. Biotechnol J 10 (2015), 1170–1184.
    • (2015) Biotechnol J , vol.10 , pp. 1170-1184
    • Heider, S.A.1    Wendisch, V.F.2
  • 73
    • 84887624541 scopus 로고    scopus 로고
    • Frontiers of yeast metabolic engineering: diversifying beyond ethanol and Saccharomyces
    • 73 Liu, L., Redden, H., Alper, H.S., Frontiers of yeast metabolic engineering: diversifying beyond ethanol and Saccharomyces. Curr Opin Biotechnol 24 (2013), 1023–1030.
    • (2013) Curr Opin Biotechnol , vol.24 , pp. 1023-1030
    • Liu, L.1    Redden, H.2    Alper, H.S.3
  • 74
    • 70449715238 scopus 로고    scopus 로고
    • Production of aromatic compounds in bacteria
    • 74 Gosset, G., Production of aromatic compounds in bacteria. Curr Opin Biotechnol 20 (2009), 651–658.
    • (2009) Curr Opin Biotechnol , vol.20 , pp. 651-658
    • Gosset, G.1
  • 75
    • 0141594681 scopus 로고    scopus 로고
    • Heterologous production of flavanones in Escherichia coli: potential for combinatorial biosynthesis of flavonoids in bacteria
    • 75 Kaneko, M., Hwang, E.I., Ohnishi, Y., Horinouchi, S., Heterologous production of flavanones in Escherichia coli: potential for combinatorial biosynthesis of flavonoids in bacteria. J Ind Microbiol Biotechnol 30 (2003), 456–461.
    • (2003) J Ind Microbiol Biotechnol , vol.30 , pp. 456-461
    • Kaneko, M.1    Hwang, E.I.2    Ohnishi, Y.3    Horinouchi, S.4
  • 76
    • 84928749665 scopus 로고    scopus 로고
    • Biological synthesis of coumarins in Escherichia coli
    • 76 Yang, S.M., Shim, G.Y., Kim, B.G., Ahn, J.H., Biological synthesis of coumarins in Escherichia coli. Microb Cell Fact, 14, 2015, 65.
    • (2015) Microb Cell Fact , vol.14 , pp. 65
    • Yang, S.M.1    Shim, G.Y.2    Kim, B.G.3    Ahn, J.H.4
  • 77
    • 84930201218 scopus 로고    scopus 로고
    • Enabling technologies to advance microbial isoprenoid production
    • 77 Chen, Y., Zhou, Y.J., Siewers, V., Nielsen, J., Enabling technologies to advance microbial isoprenoid production. Adv Biochem Eng Biotechnol 148 (2015), 143–160.
    • (2015) Adv Biochem Eng Biotechnol , vol.148 , pp. 143-160
    • Chen, Y.1    Zhou, Y.J.2    Siewers, V.3    Nielsen, J.4
  • 78
    • 84942774964 scopus 로고    scopus 로고
    • Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli
    • 78 Kong, M.K., Kang, H.J., Kim, J.H., Oh, S.H., Lee, P.C., Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli. J Biotechnol 214 (2015), 95–102.
    • (2015) J Biotechnol , vol.214 , pp. 95-102
    • Kong, M.K.1    Kang, H.J.2    Kim, J.H.3    Oh, S.H.4    Lee, P.C.5
  • 79
    • 84955267195 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli for the production of cinnamaldehyde
    • 79 Bang, H.B., Lee, Y.H., Kim, S.C., Sung, C.K., Jeong, K.J., Metabolic engineering of Escherichia coli for the production of cinnamaldehyde. Microb Cell Fact, 15, 2016, 16.
    • (2016) Microb Cell Fact , vol.15 , pp. 16
    • Bang, H.B.1    Lee, Y.H.2    Kim, S.C.3    Sung, C.K.4    Jeong, K.J.5
  • 80
    • 84901617508 scopus 로고    scopus 로고
    • In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli
    • 80 Zhu, F., Zhong, X., Hu, M., Lu, L., Deng, Z., Liu, T., In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli. Biotechnol Bioeng 111 (2014), 1396–1405.
    • (2014) Biotechnol Bioeng , vol.111 , pp. 1396-1405
    • Zhu, F.1    Zhong, X.2    Hu, M.3    Lu, L.4    Deng, Z.5    Liu, T.6
  • 81
    • 84877256074 scopus 로고    scopus 로고
    • Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols
    • 81 Avalos, J.L., Fink, G.R., Stephanopoulos, G., Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols. Nat Biotechnol 31 (2013), 335–341.
    • (2013) Nat Biotechnol , vol.31 , pp. 335-341
    • Avalos, J.L.1    Fink, G.R.2    Stephanopoulos, G.3
  • 83
    • 84900032261 scopus 로고    scopus 로고
    • Narrowing the gap between the promise and reality of polyketide synthases as a synthetic biology platform
    • 83 Poust, S., Hagen, A., Katz, L., Keasling, J.D., Narrowing the gap between the promise and reality of polyketide synthases as a synthetic biology platform. Curr Opin Biotechnol 30 (2014), 32–39.
    • (2014) Curr Opin Biotechnol , vol.30 , pp. 32-39
    • Poust, S.1    Hagen, A.2    Katz, L.3    Keasling, J.D.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.