Systems metabolic engineering of Escherichia coli for gram scale production of the antitumor drug deoxyviolacein from glycerol

Biotechnology and Bioengineering

Volumn 111, Issue 11, 2014, Pages 2280-2289

  Rodrigues, André Luis ^a,b   Becker, Judith ^a   de Souza Lima, André Oliveira ^c   Porto, Luismar Marques ^d   Wittmann, Christoph ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^c UNIVERSIDADE DO VALE DO ITAJAÍ   (Brazil)

^d FEDERAL UNIVERSITY OF SANTA CATARINA   (Brazil)

Author keywords

13C flux; Deoxyviolacein; Glycerol; L arabinose; Natural product; Tryptophan

Indexed keywords

AMINO ACIDS; BACTERIA; BATCH DATA PROCESSING; BIOACTIVITY; BIOCHEMISTRY; BIOSYNTHESIS; CARBON; DIAGNOSIS; GENES; GLYCEROL; ISOTOPES; METABOLIC ENGINEERING; METABOLISM; TUMORS;

DEOXYVIOLACEIN; GRAM-POSITIVE BACTERIUM; HETEROLOGOUS PRODUCTION; L-ARABINOSE; NATURAL PRODUCTS; PENTOSE PHOSPHATE PATHWAY; SYSTEMS METABOLIC ENGINEERINGS; TRYPTOPHAN;

ESCHERICHIA COLI;

ACETIC ACID; ANTINEOPLASTIC AGENT; ARABINOSE; BACTERIAL EXTRACT; CARBOHYDRATE; DEOXYVIOLACEIN; GLYCEROL; TRYPTOPHAN; UNCLASSIFIED DRUG; INDOLE DERIVATIVE; VIOLACEIN;

ARTICLE; BATCH PROCESS; CARBON SOURCE; ESCHERICHIA COLI; GENE DELETION; GENETIC ENGINEERING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION MONITORING; MASS FRAGMENTOGRAPHY; METABOLIC ENGINEERING; NONHUMAN; OPERON; PENTOSE PHOSPHATE CYCLE; REPRESSOR GENE; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR CLONING; MULTIGENE FAMILY; PLASMID; PROCEDURES; PROMOTER REGION;

ESCHERICHIA COLI; POSIBACTERIA;

ANTINEOPLASTIC AGENTS; ARABINOSE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GLYCEROL; INDOLES; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; MULTIGENE FAMILY; PLASMIDS; PROMOTER REGIONS, GENETIC;

EID: 84923923224     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25297     Document Type: Article

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