Enhanced production of 2'-fucosyllactose in engineered Escherichia coli BL21star(DE3) by modulation of lactose metabolism and fucosyltransferase

Journal of Biotechnology

Volumn 210, Issue , 2015, Pages 107-115

  Chin, Young Wook ^a   Kim, Ji Yeong ^a   Lee, Won Heong ^b   Seo, Jin Ho ^a  

^a Seoul National University   (South Korea)

^b Chonnam National University   (South Korea)

Author keywords

2' Fucosyllactose; Aspartate tag; Engineered E. coli; GDP l fucose; Lac operon

Indexed keywords

BIOSYNTHESIS; ENZYMES; FERMENTATION; GENE EXPRESSION; SUGARS;

ASPARTATES; E. COLI; FUCOSYLLACTOSE; GDP-L-FUCOSE; LAC OPERON;

ESCHERICHIA COLI;

2' FUCOSYLLACTOSE; ASPARTIC ACID; BETA GALACTOSIDASE; FUCOSYLTRANSFERASE; GUANOSINE DIPHOSPHATE FUCOSE; LACTOSE; OLIGOSACCHARIDE; UNCLASSIFIED DRUG; 2'-FUCOSYLLACTOSE; ESCHERICHIA COLI PROTEIN; RECOMBINANT PROTEIN; TRISACCHARIDE;

ARTICLE; CARBOHYDRATE METABOLISM; ESCHERICHIA COLI; FED BATCH FERMENTATION; GENE EXPRESSION; GENETIC ENGINEERING; HELICOBACTER PYLORI; LACTOSE OPERON; NONHUMAN; PRIORITY JOURNAL; BATCH CELL CULTURE; BIOSYNTHESIS; FERMENTATION; GENE DELETION; GENETICS; HUMAN; METABOLISM; MICROBIOLOGY; PROCEDURES;

ESCHERICHIA COLI; ESCHERICHIA COLI K12; HELICOBACTER PYLORI;

BATCH CELL CULTURE TECHNIQUES; BETA-GALACTOSIDASE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERMENTATION; FUCOSYLTRANSFERASES; GENE DELETION; HUMANS; INDUSTRIAL MICROBIOLOGY; LAC OPERON; LACTOSE; RECOMBINANT PROTEINS; TRISACCHARIDES;

EID: 85047696209     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2015.06.431     Document Type: Article

References (32)

1. Albermann C., Piepersberg W., Wehmeier U. Synthesis of the milk oligosaccharide 2'-fucosyllactose using recombinant bacterial enzymes. Carbohyd. Res. 2001, 334:97-103.
2. Baumgärtner F., Conrad J., Sprenger G.A., Albermann C. Synthesis of the human milk oligosaccharide lacto-N-tetraose in metabolically engineered plasmid-free E. coli. Chembiochem 2014, 15:1896-1900.
3. Baumgärtner F., Seitz L., Sprenger G.A., Albermann C. Construction of E. coli strains with chromosomally integrated expression cassettes for the synthesis of 2'-fucosyllactose. Microb. Cell Fac. 2013, 12:40.
4. Bode L. Human milk oligosaccharides: every baby needs a sugar mama. Glycobiology 2012, 22:1147-1162.
5. Castanys-Muñoz E., Martin M.J., Prieto P.A. 2'-fucosyllactose: an abundant, genetically determined soluble glycan present in human milk. Nutr. Rev. 2013, 71(12):773-789.
6. Datsenko K.A., Wanner B.L. One-step inactivation of chromosomal genes in E. coli K-12 using PCR products. Proc. Natl. Acad. Sci. U. S. A 2000, 97:6640-6645.
7. Drouillard S., Driguez H., Samain E. Large-scale synthesis of H-antigen oligosaccharides by expressing Helicobacter pylori α-1,2-fucosyltransferase in metabolically engineered E. coli cells. Angew. Chem. 2006, 118:1810-1812.
8. Dumon C., Samain E., Priem B. Assessment of the two Helicobacter pylori alpha-1, 3-fucosyltransferase ortholog genes for the large-scale synthesis of LewisX human milk oligosaccharides by metabolically engineered E. coli. Biotechnol. Prog. 2004, 20(2):412-419.
9. Engels L., Elling L. WbgL: a novel bacterial α1, 2-fucosyltransferase for the synthesis of 2'-fucosyllactose. Glycobiology 2014, 24:170-178.
10. Ghigo J.-M. Natural conjugative plasmids induce bacterial biofilm development. Nature 2001, 412:442-445.
11. Han N.S., Kim T.J., Park Y.C., Kim J., Seo J.H. Biotechnological production of human milk oligosaccharides. Biotechnol. Adv. 2012, 30:1268-1278.
12. Jung H.-J., Kim S.-K., Min W.-K., Lee S.-S., Park K., Park Y.-C., Seo J.-H. Polycationic amino acid tags enhance soluble expression of Candida antarctica lipase B in recombinant E. coli. Bioproc. Biosys. Eng. 2011, 34:833-839.
13. Kato A., Maki K., Ebina T., Kuwajima K., Soda K., Kuroda Y. Mutational analysis of protein solubility enhancement using short peptide tags. Biopolymers 2007, 85:12-18.
14. Kim S.K., Park Y.C., Lee H.H., Jeon S.T., Min W.K., Seo J.H. Simple amino acid tags improve both expression and secretion of Candida antarctica lipase B in recombinant E. coli. Biotechnol. Bioeng. 2015, 112(2):346-355.
15. Kweon D.H., Kim S.G., Han N.S., Lee J.H., Chung K.M., Seo J.H. Immobilization of Bacillus macerans cyclodextrin glycosyltransferase fused with poly-lysine using cation exchanger. Enzyme Microb. Tech. 2005, 36:571-578.
16. Langley K.E., Fowler A.V., Zabin I. Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3-92. J. Biol. Chem. 1975, 250:2587-2592.
17. Lee J., Kim D., Sohng J. Cloning and functional characterization of an α-1,3-fucosyltransferase from Bacteroides fragilis. Biotechnol. Bioprocess Eng. 2013, 18(5):843-849.
18. Lee W.H., Han N.S., Park Y.C., Seo J.H. Modulation of guanosine 5'-diphosphate-d-mannose metabolism in recombinant E. coli for production of guanosine 5'-diphosphate-l-fucose. Bioresource Technol. 2009, 100:6143-6148.
19. Lee W.H., Pathanibul P., Quarterman J., Jo J.H., Han N.S., Miller M.J., Jin Y.S., Seo J.H. Whole cell biosynthesis of a functional oligosaccharide, 2'-fucosyllactose, using engineered E. coli. Microb. Cell Fac. 2012, 11:48.
20. Malamy, M.H., 1966. Frameshift mutations in the lactose operon of E. coli. Cold Spring Harbor symposia on quantitative biology. Cold Spring Harbor Laboratory Press, 189-201.
21. Mattila-Sandholm T., Wirtanen G. Biofilm formation in the industry: a review. Food Rev. Int. 1992, 8:573-603.
22. McKenzie G.J., Craig N.L. Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event. BMC Microbial. 2006, 6. 39.
23. Phue J.N., Lee S.J., Trinh L., Shiloach J. Modified E. coli B (BL21), a superior producer of plasmid DNA compared with E. coli K (DH5α). Biotechnol. Bioeng. 2008, 101:831-836.
24. Postma P., Lengeler J., Jacobson G. Phosphoenolpyruvate: carbohydrate phosphotransferase systems of bacteria. Microbiol. Rev. 1993, 57:543.
25. Ren D., Zuo R., Barrios A.F.G., Bedzyk L.A., Eldridge G.R., Pasmore M.E., Wood T.K. Differential gene expression for investigation of E. coli biofilm inhibition by plant extract ursolic acid. Appl. Environ. Microbiol. 2005, 71:4022-4034.
26. Shiloach J., Kaufman J., Guillard A., Fass R. Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by E. coli BL21 ((DE3) and E. coli JM109. Biotechnol. Bioeng. 1996, 49:421-428.
27. Son Y.-J., Phue J.-N., Trinh L.B., Lee S.J., Shiloach J. The role of Cra in regulating acetate excretion and osmotic tolerance in E. coli K-12 and E. coli B at high density growth. Microb. Cell Fac. 2011, 10:52.
28. Teodósio J., Simões M., Mergulhão F. The influence of nonconjugative E. coli plasmids on biofilm formation and resistance. J. Appl. Microbiol. 2012, 113:373-382.
29. Wang G., Boulton P.G., Chan N.W.C., Palcic M.M., Taylor D.E. Novel Helicobacter pylori α1, 2-fucosyltransferase, a key enzyme in the synthesis of Lewis antigens. Microbiology 1999, 145:3245-3253.
30. Waugh D.S. Making the most of affinity tags. Trends Biotechnol. 2005, 23:316-320.
31. Xia X.X., Han M.J., Lee S.Y., Yoo J.S. Comparison of the extracellular proteomes of E. coli B and K-12 strains during high cell density cultivation. Proteomics 2008, 8:2089-2103.
32. Yazdani S.S., Gonzalez R. Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr. Opin. Biotech. 2007, 18:213-219.