A high-throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors

Biotechnology and Bioengineering

Volumn 101, Issue 4, 2008, Pages 788-796

  Yu, Huimin ^a,b   Tyo, Keith ^a   Alper, Hal ^a   Klein Marcuschamer, Daniel ^a   Stephanopoulos, Gregory ^a  

^a USA   (United States)

^b TSINGHUA UNIVERSITY   (China)

Author keywords

Alcian blue staining; Global transcriptional machinery engineering; High throughput screening; Hyaluronic acid; Sigma factor engineering; Translucent colony identification

Indexed keywords

ABS RESINS; ACIDS; CELL CULTURE; CONCENTRATION (PROCESS); ESCHERICHIA COLI; HYDROXYLATION; LIBRARIES; MACHINERY; NUCLEIC ACIDS; ORGANIC ACIDS; POLYSACCHARIDES; PROGRAMMING THEORY; RNA; STRAIN; THROUGHPUT;

ALCIAN BLUE STAINING; GLOBAL TRANSCRIPTIONAL MACHINERY ENGINEERING; HIGH THROUGHPUT SCREENING; HYALURONIC ACID; SIGMA FACTOR ENGINEERING; TRANSLUCENT COLONY IDENTIFICATION;

BIOLOGICAL MATERIALS;

ALCIAN BLUE; HYALURONIC ACID; RNA POLYMERASE;

ARTICLE; BACTERIAL STRAIN; BACTERIUM COLONY; BACTERIUM IDENTIFICATION; BACTERIUM TRANSFORMATION; CONCENTRATION (PARAMETERS); ESCHERICHIA COLI; GENE LIBRARY; GENE MUTATION; MUTANT; NONHUMAN; PHOTOMETRY; SCREENING;

ESCHERICHIA COLI; GENE LIBRARY; HYALURONIC ACID; MUTANT PROTEINS; MUTATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE DELETION; SIGMA FACTOR; STAINING AND LABELING; TRANSFORMATION, GENETIC;

ESCHERICHIA COLI;

EID: 55549129643     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21947     Document Type: Article

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