Designer amphiphilic proteins as building blocks for the intracellular formation of organelle-like compartments

Nature Materials

Volumn 14, Issue 1, 2015, Pages 125-132

  Huber, Matthias C ^a   Schreiber, Andreas ^a   Von Olshausen, Philipp ^a   Varga, Balázs R ^b   Kretz, Oliver ^a   Joch, Barbara ^a   Barnert, Sabine ^a   Schubert, Rolf ^a   Eimer, Stefan ^a   Kele, Péter ^b   Schiller, Stefan M ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOLOGICAL MATERIALS; BIOLOGICAL MEMBRANES; BIOSYNTHESIS; CHEMICAL MODIFICATION; ENZYME ACTIVITY; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; MACHINERY; REFRACTIVE INDEX; TRANSMISSION ELECTRON MICROSCOPY;

CELLULAR COMPARTMENTS; CELLULAR FUNCTION; CELLULAR MACHINERY; CHEMICAL FUNCTIONALITY; EPIFLUORESCENCE MICROSCOPY; PROTEIN STRUCTURES; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; UNNATURAL AMINO ACIDS;

PROTEINS;

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN; RECOMBINANT PROTEIN;

CELL ORGANELLE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; METABOLISM; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; MEMBRANE PROTEINS; MICROSCOPY, ELECTRON, TRANSMISSION; ORGANELLES; RECOMBINANT PROTEINS;

EID: 84928756896     PISSN: 14761122     EISSN: 14764660     Source Type: Journal    
DOI: 10.1038/nmat4118     Document Type: Article

References (42)

1. Shively, J. M., Decker, G. L. & Greenawalt, J.W. Comparative ultrastructure of the thiobacilli. J. Bacteriol. 101, 618-627 (1970).
2. Shively, J. M., Ball, F., Brown, D. H. & Saunders, R. E. Functional organelles in prokaryotes: Polyhedral inclusions (carboxysomes) of Thiobacillus neapolitanus. Science 182, 584-586 (1973).
3. Yeates, T. O., Kerfeld, C. A., Heinhorst, S., Cannon, G. C. & Shively, J. M. Protein-based organelles in bacteria: Carboxysomes and related microcompartments. Nature Rev. Microbiol. 6, 681-691 (2008).
4. Kerfeld, C. A., Heinhorst, S. & Cannon, G. C. Bacterial microcompartments. Annu. Rev. Microbiol. 64, 391-408 (2010).
5. Cheng, S., Liu, Y., Crowley, C. S., Yeates, T. O. & Bobik, T. A. Bacterial microcompartments: Their properties and paradoxes. BioEssays 30, 1084-1095 (2008).
6. Roodbeen, R. & Van Hest, J. C. M. Synthetic cells and organelles: Compartmentalization strategies. BioEssays 31, 1299-1308 (2009).
7. Parsons, J. B. et al. Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Mol. Cell 38, 305-315 (2010).
8. Vargo, K. B., Parthasarathy, R. & Hammer, D. A. Self-assembly of tunable protein suprastructures from recombinant oleosin. Proc. Natl Acad. Sci. USA 109, 11657-11662 (2012).
9. Kim,W., Thévenot, J., Ibarboure, E., Lecommandoux, S. & Chaikof, E. L. Self-assembly of thermally responsive amphiphilic diblock copolypeptides into spherical micellar nanoparticles. Angew. Chem. Int. Ed. 49, 4257-4260 (2010).
10. Bellomo, E. G.,Wyrsta, M. D., Pakstis, L., Pochan, D. J. & Deming, T. J. Stimuli-responsive polypeptide vesicles by conformation-specific assembly. Nature Mater. 3, 244-248 (2004).
11. Martín, L., Castro, E., Ribeiro, A., Alonso, M. & Rodríguez-Cabello, J. C. Temperature-triggered self-assembly of elastin-like block co-recombinamers: The controlled formation of micelles and vesicles in an aqueous medium. Biomacromolecules 13, 293-298 (2012).
12. Agapakis, C. M., Boyle, P. M. & Silver, P. A. Natural strategies for the spatial optimization of metabolism in synthetic biology. Nature Chem. Biol. 8, 527-535 (2012).
13. Medema, M. H., van, R. R., Takano, E. & Breitling, R. Computational tools for the synthetic design of biochemical pathways. Nature Rev. Microbiol. 10, 191-202 (2012).
14. Dueber, J. E. et al. Synthetic protein scaffolds provide modular control over metabolic flux. Nature Biotechnol. 27, 753-759 (2009).
15. Medema, M. H., Breitling, R., Bovenberg, R. & Takano, E. Exploiting plug-and-play synthetic biology for drug discovery and production in microorganisms. Nature Rev. Microbiol. 9, 131-137 (2011).
16. LeDuc, P. R. et al. Towards an in vivo biologically inspired nanofactory. Nature Nanotech. 2, 3-7 (2007).
17. Israelachvili, J. N. Intermolecular and Surface Forces 3rd edn (Academic Press, 2011).
18. Cho, Y. et al. Hydrogen bonding of turn structure is stabilized in D2O. J. Am. Chem. Soc. 131, 15188-15193 (2009).
19. Kurková, D. et al. Structure and dynamics of two elastin-like polypentapeptides studied by NMR spectroscopy. Biomacromolecules 4, 589-601 (2003).
20. Dreher, M. R. et al. Temperature triggered self-assembly of polypeptides into multivalent spherical micelles. J. Am. Chem. Soc. 130, 687-694 (2008).
21. Lee, T. A. T., Cooper, A., Apkarian, R. P. & Conticello, V. P. Thermo-reversible self-assembly of nanoparticles derived from elastin-mimetic polypeptides. Adv. Mater. 12, 1105-1110 (2000).
22. Urry, D.W. et al. Elastin: A representative ideal protein elastomer. Phil. Trans. R. Soc. Lond. B 357, 169-184 (2002).
23. Lee, S. C., Choi, Y. C. & Yu, M-H. Effect of the N-terminal hydrophobic sequence of hepatitis B virus surface antigen on the folding and assembly of hybrid galactosidase in Escherichia coli. Eur. J. Biochem. 187, 417-424 (1990).
24. Cabrita, L. D., Dobson, C. M. & Christodoulou, J. Protein folding on the ribosome. Curr. Opin. Struct. Biol. 20, 33-45 (2010).
25. Vanhecke, D., Graber,W. & Studer, D. in Methods in Cell Biology Vol. 88 (ed. Allen, T. D.) 151-164 (Academic Press, 2008).
26. Hurbain, I. & Sachse, M. The future is cold: Cryo-preparation methods for transmission electron microscopy of cells. Biol. Cell 103, 405-420 (2011).
27. McDonald, K. & Auer, M. High-pressure freezing, cellular tomography, and structural cell biology. BioTechniques 41, 137-143 (2006).
28. Walde, P.,Wick, R., Fresta, M., Mangone, A. & Luisi, P. L. Autopoietic self-reproduction of fatty acid vesicles. J. Am. Chem. Soc. 116, 11649-11654 (1994).
29. Shimamoto, T. et al. The NhaB NaC/HC antiporter is essential for intracellular pH regulation under alkaline conditions in Escherichia coli. J. Biochem. 116, 285-290 (1994).
30. Record, M. T. Jr, Courtenay, E. S., Cayley, D. S. & Guttman, H. J. Responses of E. coli to osmotic stress: Large changes in amounts of cytoplasmic solutes and water. Trends Biochem. Sci. 23, 143-148 (1998).
31. Chen, I. A. &Walde, P. From self-assembled vesicles to protocells. Cold Spring Harb. Perspect. Biol. 2 (2010).
32. Hirvonen, L.,Wicker, K., Mandula, O. & Heintzmann, R. Structured illumination microscopy of a living cell. Eur. Biophys. J. 38, 807-812 (2009).
33. Dash, B. C. et al. Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot. J. Control. Release 152, 382-392 (2011).
34. Chin, J.W. et al. Addition of p-Azido-L-phenylalanine to the genetic code of Escherichia coli. J. Am. Chem. Soc. 124, 9026-9027 (2002).
35. Wang, L., Brock, A., Herberich, B. & Schultz, P. G. Expanding the genetic code of Escherichia coli. Science 292, 498-500 (2001).
36. Laughlin, S. T., Baskin, J. M., Amacher, S. L. & Bertozzi, C. R. In vivo imaging of membrane-associated glycans in developing zebrafish. Science 320, 664-667 (2008).
37. Varga, B. R., Kállay, M., Hegyi, K., Béni, S. & Kele, P. A non-fluorinated monobenzocyclooctyne for rapid copper-free click reactions. Chem. Eur. J. 18, 822-828 (2012).
38. Schreiber, A. & Schiller, S. M. Nanobiotechnology of protein compartments: Steps towards nanofactories. Bioinsp. Biomim. Nanobiomater. 4, 157-164 (2013).
39. Baneyx, F. Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol. 10, 411-421 (1999).
40. Glick, B. R. Metabolic load and heterologous gene expression. Biotechnol. Adv. 13, 247-261 (1995).
41. Huber, M. C. et al. Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid-materials. Biomaterials 35, 8767-8779 (2014).
42. Schrand, A. M., Schlager, J. J., Dai, L. & Hussain, S. M. Preparation of cells for assessing ultrastructural localization of nanoparticles with transmission electron microscopy. Nature Protocols 5, 744-757 (2010).