Quantification of MFGM proteins in buttermilk and butter serum by means of a stain free SDS-PAGE method

Journal of Food Composition and Analysis

Volumn 49, Issue , 2016, Pages 102-109

  Holzmüller, Wolfgang ^a   Kulozik, Ulrich ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Butter serum; Buttermilk; MFGM proteins; Protein quantification; SDS PAGE; Stain free technology; Tryptophan

Indexed keywords

EID: 84966377278     PISSN: 08891575     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jfca.2016.04.003     Document Type: Article

References (52)

1. Anderson M., Cawston T.E. Reviews of the progress of dairy science: the milk fat globule membrane. J. Dairy Res. 1975, 42:459-483.
2. Bobe G., Beitz D.C., Freeman A.E., Lindberg G.L. Separation and quantification of bovine milk proteins by reversed-phase high-performance liquid chromatography. J. Agric. Food Chem. 1998, 46:458-463.
3. Britten M., Lamothe S., Robitaille G. Effect of cream treatment on phospholipids and protein recovery in butter-making process. Int. J. Food Sci. Technol. 2008, 43:651-657.
4. Brown D.A. Lipid droplets: proteins floating on a pool of fat. Curr. Biol. 2001, 11:446-449.
5. Corredig M., Dalgleish D.G. Isolates from industrial buttermilk: emulsifying properties of materials derived from the milk fat globule membrane. J. Agric. Food Chem. 1997, 45:4595-4600.
6. Danthine S., Blecker C., Paquot M., Innocente N., Deroanne C. Progress in milk fat globule membrane research: a review. Évolution des connaissances sur la membrane du globule gras du lait Synthèse bibliographique. Lait 2000, 80:209-222.
7. Deeth H.C. The role of phospholipids in the stability of milk fat globules. Aust. J. Dairy Technol. 1997, 52:44-46.
8. Dewettinck K., Rombaut R., Thienpont N., Le T.T., Messens K., van Camp J. Nutritional and technological aspects of milk fat globule membrane material: Netherlands Association for the Advancement of Dairy Science 1908-2008. Int. Dairy J. 2008, 18:436-457.
9. Edwards R.A., Jickling G., Turner R.J. The light-induced reactions of tryptophan with halocompounds. Photochem. Photobiol. 2002, 75:362-368.
10. Evers J.M., Haverkamp R.G., Holroyd S.E., Jameson G.B., Mackenzie D.D.S., McCarthy O.J. Heterogeneity of milk fat globule membrane structure and composition as observed using fluorescence microscopy techniques. Int. Dairy J. 2008, 18:1081-1089.
11. Freeby, S., Liu, N., Mcdonald, K., Paulus, A., Posch, A., 2013. Stain-free protein quantification and normalization. Google Patents from: . http://www.google.com/patents/WO2013163451A1%3Fcl=en.
12. Guggenmos J., Schubart A.S., Ogg S., Andersson M., Olsson T., Mather I.H., Linington C. Antibody cross-reactivity between myelin oligodendrocyte glycoprotein and the milk protein butyrophilin in multiple sclerosis. J. Immunol. 2004, 172:661-668.
13. Hancock J.T., Salisbury V., Ovejero-Boglione M.C., Cherry R., Hoare C., Eisenthal R., Harrison R. Antimicrobial properties of milk: dependence on presence of xanthine oxidase and nitrite. Antimicrob. Agents Chemother. 2002, 46:3308-3310.
14. Holzmüller W., Kulozik U. Protein quantification by means of a stain-free SDS-PAGE technology without the need for analytical standards: verification and validation of the method. J. Food Compos. Anal. 2016, 48:128-134.
15. Holzmüller W., Kulozik U. Technical difficulties and future challenges in isolating membrane material from milk fat globules in industrial settings-a critical review. Int. Dairy J. 2016, (submitted for publication).
16. Holzmüller W., Müller M., Himbert D., Kulozik U. Impact of cream washing on fat globules and milk fat globule membrane proteins. Int. Dairy J. 2016, 59:52-61.
17. Houlihan A.V., Goddard P.A., Kitchen B.J., Masters C.J. Changes in structure of the bovine milk fat globule membrane on heating whole milk. J. Dairy Res. 1992, 59:321-329.
18. Jones D.B. Factors for Converting Percentages of Nitrogen in Foods and Feeds into Percentages of Proteins: Circular No. 183 1941, U.S. Department of Agriculture, Washington D.C.
19. Keenan T.W., Mather I.H. Intracellular origin of milk fat globules and the nature of the milk fat globule membrane. Advanced Dairy Chemistry: Lipids 2006, vol. 2:137-171. Springer, New York.
20. Keenan T.W., Patton S. The structure of milk: implications for sampling and storage. A. The milk lipid globule membrane. Handbook of Milk Composition 1995, 4-49. Academic Press, San Diego, New York, Boston. R.G. Jensen (Ed.).
21. Ladner C.L., Yang J., Turner R.J., Edwards R.A. Visible fluorescent detection of proteins in polyacrylamide gels without staining. Anal. Biochem. 2004, 326:13-20.
22. Ladner C.L., Tran K., Le M., Turner R.J., Edwards R.A. Excited state photoreaction between the indole side chain of tryptophan and halocompounds generates new fluorophores and unique modifications. Photochem. Photobiol. 2014, 90:1027-1033.
23. Lamothe S., Robitaille G., St-Gelais D., Britten M. Butter making from caprine creams: effect of washing treatment on phospholipids and milk fat globule membrane proteins distribution. J. Dairy Res. 2008, 75:439-443.
24. Le T.T., van Camp J., Rombaut R., van Leeckwyck F., Dewettinck K. Effect of washing conditions on the recovery of milk fat globule membrane proteins during the isolation of milk fat globule membrane from milk. J. Dairy Sci. 2009, 92:3592-3603.
25. Le T.T., Debyser G., Gilbert W., Struijs K., van Camp J., van de Wiele T., Devreese B., Dewettinck K. Distribution and isolation of milk fat globule membrane proteins during dairy processing as revealed by proteomic analysis. Int. Dairy J. 2013, 32:110-120.
26. Lopez C., Madec M.N., Jimenez-Flores R. Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins. Food Chem. 2010, 120:22-33.
27. Mather I.H. A review and proposed nomenclature for major proteins of the milk-fat globule membrane. J. Dairy Sci. 2000, 83:203-247.
28. Mather I.H., Weber K., Keenan T.W. Membranes of mammary gland. XII. Loosely associated proteins and compositional heterogeneity of bovine milk fat globule membrane. J. Dairy Sci. 1977, 60:394-402.
29. Moller H.J., Heinegard D., Poulsen J.H. Combined alcian blue and silver staining of subnanogram quantities of proteoglycans and glycosaminoglycans in sodium dodecyl sulfate-polyacrylamide gels. Anal. Biochem. 1993, 209:169-175.
30. Mondy B.L., Keenan T.W. Butyrophilin and xanthine oxidase occur in constant molar proportions in milk lipid globule membrane but vary in amount with breed and stage of lactation. Protoplasma 1993, 177:32-36.
31. Morin P., Britten M., Jiménez-Flores R., Pouliot Y. Microfiltration of buttermilk and washed cream buttermilk for concentration of milk fat globule membrane components. J. Dairy Sci. 2007, 90:2132-2140.
32. Morin P., Jiménez-Flores R., Pouliot Y. Effect of processing on the composition and microstructure of buttermilk and its milk fat globule membranes. Int. Dairy J. 2007, 17:1179-1187.
33. Mulder H., Walstra P. The Milk Fat Globule: Emulsion Science as Applied to Milk Products and Comparable Foods 1974, Commonwealth Agricultural Bureaux, Slough.
34. Nielsen R.L., Andersen M.H., Mabhout P., Berglund L., Petersen T.E., Rasmussen J.T. Isolation of adipophilin and butyrophilin from bovine milk and characterization of a cDNA encoding adipophilin. J. Dairy Sci. 1999, 82:2543-2549.
35. Osset M., Piñol M., Fallon M.J.M., Llorens R., de Cuchillo C.M. Interference of the carbohydrate moiety in Coomassie Brilliant Blue R-250 protein staining. Electrophoresis 1989, 10:271-273.
36. Patton S., Huston G. A method for isolation of milk fat globules. Lipids 1986, 21:170-174.
37. Riccio P. The proteins of the milk fat globule membrane in the balance. Trends Food Sci. Technol. 2004, 15:458-461.
38. Rombaut R., Camp J.V., Dewettinck K. Analysis of phospho- and sphingolipids in dairy products by a new HPLC method. J. Dairy Sci. 2005, 88:482-488.
39. Rombaut R., Dejonckheere V., Dewettinck K. Microfiltration of butter serum upon casein micelle destabilization. J. Dairy Sci. 2006, 89:1915-1925.
40. Rombaut R., van Camp J., Dewettinck K. Phospho- and sphingolipid distribution during processing of milk: butter and whey. Int. J. Food Sci. Technol. 2006, 41:435-443.
41. Rombaut R., Dewettinck K., van Camp J. Phospho- and sphingolipid content of selected dairy products as determined by HPLC coupled to an evaporative light scattering detector (HPLC-ELSD): the essential balance: risks and benefits in food safety and quality. J. Food Compos. Anal. 2007, 20:308-312.
42. Shimizu M., Kanno C., Yamauchi K. Selective solubilization of bovine milk fat globule membrane proteins with guanidine hydrochloride and disposition of some of the proteins. Agric. Biol. Chem. 1978, 42:2309-2314.
43. Singh H. The milk fat globule membrane-a biophysical system for food applications. Curr. Opin. Colloid Interface Sci. 2006, 11:154-163.
44. Spitsberg V.L. Invited review: bovine milk fat globule membrane as a potential nutraceutical. J. Dairy Sci. 2005, 88:2289-2294.
45. Spitsberg V.L., Gorewit R.C. In vitro phosphorylated bovine milk fat globule membrane proteins. J. Nutr. Biochem. 1997, 8:181-189.
46. Tal M., Silberstein A., Nusser E. Why does Coomassie Brilliant Blue R interact differently with different proteins? A partial answer. J. Biol. Chem. 1985, 260:9976-9980.
47. Vanderghem C., Francis F., Danthine S., Deroanne C., Paquot M., Pauw E., de Blecker C. Study on the susceptibility of the bovine milk fat globule membrane proteins to enzymatic hydrolysis and organization of some of the proteins. Int. Dairy J. 2011, 21:312-318.
48. Vojdani A., Campbell A.W., Anyanwu E., Kashanian A., Bock K., Vojdani E. Antibodies to neuron-specific antigens in children with autism: possible cross-reaction with encephalitogenic proteins from milk, Chlamydia pneumoniae and Streptococcus group A. J. Neuroimmunol. 2002, 129:168-177.
49. Walstra P., Wouters J.T.M., Geurts T.J. Dairy Science and Technology 2006, Taylor & Francis, Boca Raton, FL. 2nd ed.
50. Ye A., Anema S.G., Singh H. High-pressure-induced interactions between milk fat globule membrane proteins and skim milk proteins in whole milk. J. Dairy Sci. 2004, 87:4013-4022.
51. Ye A., Singh H., Taylor M.W., Anema S. Interactions of whey proteins with milk fat globule membrane proteins during heat treatment of whole milk. Lait 2004, 84:269-283.
52. Zheng H., Jiménez-Flores R., Everett D.W. Bovine milk fat globule membrane proteins are affected by centrifugal washing processes. J. Agric. Food Chem. 2013, 61:8403-8411.