Two Divalent Metal Ions and Conformational Changes Play Roles in the Hammerhead Ribozyme Cleavage Reaction

Biochemistry

Volumn 54, Issue 41, 2015, Pages 6369-6381

  Mir, Aamir ^a   Chen, Ji ^a   Robinson, Kyle ^a   Lendy, Emma ^a   Goodman, Jaclyn ^a   Neau, David ^b   Golden, Barbara L ^a  

^a PURDUE UNIVERSITY   (United States)

^b Argonne National Laboratory ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONFORMATIONS; CRYSTAL STRUCTURE; METAL IONS;

CHEMICAL MECHANISM; CLEAVAGE REACTION; CONFORMATIONAL CHANGE; DIRECT INTERACTIONS; DIVALENT METAL ION; KINETIC ANALYSIS; PH-RATE PROFILES; SOLVED CRYSTAL STRUCTURES;

METALS;

HAMMERHEAD RIBOZYME; METAL ION; NUCLEOTIDE; OXYGEN; PHOSPHATE; ZINC ION; DIVALENT CATION; HELMINTH RNA; MAGNESIUM; MANGANESE; PROTON; RIBOZYME; ZINC;

APPARENT DISSOCIATION CONSTANT; ARTICLE; BACTERIAL MUTATION; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROGEN BOND; IN VITRO STUDY; METAL BINDING; MUTAGENESIS; NONHUMAN; OBSERVED RATE CONSTANT; PH; PKA; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FUNCTION; PROTON TRANSPORT; RNA CLEAVAGE; RNA SYNTHESIS; TAUTOMER; ANIMAL; BINDING SITE; CHEMISTRY; CONFORMATION; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; MOLECULAR MODEL; NUCLEOTIDE SEQUENCE; PARASITOLOGY; POINT MUTATION; SCHISTOSOMA MANSONI; SCHISTOSOMIASIS MANSONI; X RAY CRYSTALLOGRAPHY;

ANIMALS; BASE SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; CATIONS, DIVALENT; CRYSTALLOGRAPHY, X-RAY; KINETICS; MAGNESIUM; MANGANESE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; POINT MUTATION; PROTONS; RNA, CATALYTIC; RNA, HELMINTH; SCHISTOSOMA MANSONI; SCHISTOSOMIASIS MANSONI; SUBSTRATE SPECIFICITY; ZINC;

EID: 84945322424     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.5b00824     Document Type: Article

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