Reshaping an Enzyme Binding Pocket for Enhanced and Inverted Stereoselectivity: Use of Smallest Amino Acid Alphabets in Directed Evolution

Angewandte Chemie - International Edition

Volumn 54, Issue 42, 2015, Pages 12410-12415

  Sun, Zhoutong ^a,b   Lonsdale, Richard ^a,b   Kong, Xu Dong ^c   Xu, Jian He ^c   Zhou, Jiahai ^d   Reetz, Manfred T ^a,b  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^d SHANGHAI INSTITUTE OF ORGANIC CHEMISTRY   (China)

Author keywords

amino acid alphabet; directed evolution; epoxide hydrolases; saturation mutagenesis; stereoselectivity

Indexed keywords

AMINO ACIDS; CELL IMMOBILIZATION; ENZYMES; HYDROLASES; MUTAGENESIS; RANDOM PROCESSES; STEREOSELECTIVITY;

CYCLOHEXENE OXIDE; DESYM-METRIZATION; DIRECTED EVOLUTION; EPOXIDE HYDROLASES; LIMONENE EPOXIDE HYDROLASE; ORGANIC CHEMISTRY; SATURATION MUTAGENESIS; X RAY CRYSTAL STRUCTURES;

BINDING SITES;

AMINO ACID; CYCLOHEXENE DERIVATIVE; EPOXIDE HYDROLASE; LIMONENE; TERPENE;

CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; METABOLISM; STEREOISOMERISM;

AMINO ACIDS; CYCLOHEXENES; EPOXIDE HYDROLASES; MODELS, MOLECULAR; MOLECULAR STRUCTURE; STEREOISOMERISM; TERPENES;

EID: 84943198096     PISSN: 14337851     EISSN: 15213773     Source Type: Journal    
DOI: 10.1002/anie.201501809     Document Type: Article

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