Refined regio- and stereoselective hydroxylation of l -pipecolic acid by protein engineering of l -proline cis -4-hydroxylase based on the X-ray crystal structure

ACS Synthetic Biology

Volumn 4, Issue 4, 2015, Pages 383-392

  Koketsu, Kento ^a   Shomura, Yasuhito ^b   Moriwaki, Kei ^a   Hayashi, Mikiro ^a   Mitsuhashi, Satoshi ^a   Hara, Ryotaro ^c   Kino, Kuniki ^c   Higuchi, Yoshiki ^b  

^a Kyowa Hakko Bio Co   (Japan)

^b UNIVERSITY OF HYOGO   (Japan)

^c WASEDA UNIVERSITY   (Japan)

Author keywords

hydroxy l pipecolic acid; hydroxylation; hydroxyproline; proline hydroxylase; protein engineering

Indexed keywords

PIPECOLIC ACID; PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE; BACTERIAL PROTEIN; PIPECOLIC ACID DERIVATIVE;

ARTICLE; BIOTRANSFORMATION; CATALYST; CHEMISTRY; CHIRALITY; CIS ISOMER; CRYSTAL STRUCTURE; HYDROXYLATION; ISOMER; MESORHIZOBIUM; MESORHIZOBIUM LOTI; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN ENGINEERING; PROTEIN STRUCTURE; REGIOISOMER; REGIOSELECTIVITY; SINORHIZOBIUM MELILOTI; STEREOCHEMISTRY; X RAY CRYSTALLOGRAPHY; ENZYMOLOGY; METABOLISM; PROTEIN TERTIARY STRUCTURE;

MESORHIZOBIUM LOTI; SINORHIZOBIUM MELILOTI;

BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; HYDROXYLATION; MESORHIZOBIUM; PIPECOLIC ACIDS; PROLYL HYDROXYLASES; PROTEIN STRUCTURE, TERTIARY; SINORHIZOBIUM MELILOTI;

EID: 84928151665     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb500247a     Document Type: Article

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