Substrate scope and selectivity in offspring to an enzyme subjected to directed evolution

FEBS Journal

Volumn 281, Issue 10, 2014, Pages 2387-2398

  Blikstad, Cecilia ^a   Dahlström, Käthe M ^b   Salminen, Tiina A ^b   Widersten, Mikael ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b ÅBO AKADEMI UNIVERSITY   (Finland)

Author keywords

directed evolution; kinetic characterization; oxidoreductase; structure modeling; vicinal diol

Indexed keywords

1, 2 PROPANEDIOL OXIDOREDUCTASE; 2 3 PHENYLPROPANE 1,2 DIOL; ALDEHYDE DERIVATIVE; AMINO ACID; BACTERIAL ENZYME; ISOLEUCINE; OXIDOREDUCTASE; PHENYLACETALDEHYDE; PROPYLENE GLYCOL; UNCLASSIFIED DRUG;

AMINO ACID COMPOSITION; ARTICLE; CATALYSIS; DIRECTED EVOLUTION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME METABOLISM; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; HYDROGEN BOND; MOLECULAR DOCKING; MOLECULAR EVOLUTION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; WILD TYPE;

ESCHERICHIA COLI;

DIRECTED EVOLUTION; KINETIC CHARACTERIZATION; OXIDOREDUCTASE; STRUCTURE MODELING; VICINAL DIOL;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

EID: 84901245353     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12791     Document Type: Article

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