Use of 'small but smart' libraries to enhance the enantioselectivity of an esterase from Bacillus stearothermophilus towards tetrahydrofuran-3-yl acetate

FEBS Journal

Volumn 280, Issue 13, 2013, Pages 3084-3093

  Nobili, Alberto ^a   Gall, Markus G ^a   Pavlidis, Ioannis V ^a   Thompson, Mark L ^a,b   Schmidt, Marlen ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

directed evolution; enantioselectivity; esterase; secondary alcohol; tetrahydrofuran 3 yl acetate

Indexed keywords

ACETIC ACID DERIVATIVE; ESTERASE; HYDROLASE; TETRAHYDROFURAN 3 YL ACETATE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; BIOINFORMATICS; CARBON NUCLEAR MAGNETIC RESONANCE; COMPUTER MODEL; COMPUTER PROGRAM; CONFERENCE PAPER; CONTROLLED STUDY; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME STRUCTURE; GAS CHROMATOGRAPHY; GENE MUTATION; GEOBACILLUS STEAROTHERMOPHILUS; HYDROLYSIS; MASS FRAGMENTOGRAPHY; MASS SPECTROMETRY; MOLECULAR DOCKING; MOLECULAR DYNAMICS; MOLECULAR LIBRARY; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; WILD TYPE;

ACETATES; BACTERIAL PROTEINS; CARBOXYLESTERASE; CATALYTIC DOMAIN; COMPUTATIONAL BIOLOGY; DATABASES, PROTEIN; DIRECTED MOLECULAR EVOLUTION; FURANS; GENE LIBRARY; GEOBACILLUS STEAROTHERMOPHILUS; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MOLECULAR DOCKING SIMULATION; MUTANT PROTEINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

GEOBACILLUS STEAROTHERMOPHILUS;

EID: 84879196613     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12137     Document Type: Conference Paper

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