Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Vogl, Thomas ^a,b   Ahmad, Mudassar ^a   Krainer, Florian W ^a   Schwab, Helmut ^a   Glieder, Anton ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

Author keywords

Cloning strategy; Expression optimization; Pichia pastoris; Protein tagging; Protein tags; Seamless cloning; Type IIS restriction endonucleases

Indexed keywords

HORSERADISH PEROXIDASE; HYBRID PROTEIN; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; EXPRESSED SEQUENCE TAG; FLUORESCENCE MICROSCOPY; FUNGAL STRAIN; FUNGUS IDENTIFICATION; KOMAGATAELLA PASTORIS; MOLECULAR CLONING; MOLECULAR DYNAMICS; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; RESTRICTION SITE FREE CLONING; SEQUENCE ANALYSIS; EVALUATION STUDY; GENE EXPRESSION; GENE VECTOR; GENETICS; METABOLISM; PICHIA; PROCEDURES;

ARMORACIA RUSTICANA; PICHIA PASTORIS;

CLONING, MOLECULAR; GENE EXPRESSION; GENETIC VECTORS; PICHIA; PLASMIDS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS;

EID: 84937031981     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0293-6     Document Type: Article

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