Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state

EMBO Reports

Volumn 2, Issue 9, 2001, Pages 782-786

  Besnier, C E ^a   Kong, H ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; ALANINE; CYTOSINE; DNA; DNA TOPOISOMERASE; ENDONUCLEASE; GUANINE; LYSINE; MLYI PROTEIN; MONOMER; NBI PROTEIN; NUCLEOTIDE; THYMINE; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DIMERIZATION; DNA CLEAVAGE; DNA STRAND; ESCHERICHIA COLI; GENETIC PROCEDURES; HYPOTHESIS; MOLECULAR RECOGNITION; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGOMERIZATION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, GEL; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DIMERIZATION; DNA; ESCHERICHIA COLI; KINETICS; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN BINDING; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0034769233     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1093/embo-reports/kve175     Document Type: Article

References (17)

1. N.BstSE- a site-specific nickase from Bacillus stearothermophilus SE-589
2. FokI dimerization is required for DNA cleavage
3. The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate
4. Sau3AI- a monomeric type II restriction endonuclease that dimerizes on the DNA and thereby induces DNA loops
5. Multiple DNA conformational changes induced by an initiator protein precede the nicking reaction in a rolling circle replication origin
6. The nicking endonuclease N.BstNBI is closely related to type IIs restriction endonucleases MlyI and PleI
7. Methyl-directed DNA mismatch correction
8. Characterization of the specific DNA nicking activity of restriction endonuclease N.BstNBI
9. A PCR-based strategy for extensive mutagenesis of a target DNA sequence
10. Simultaneous strand displacement amplification and fluorescence polarization detection of Chlamydia trachomatis DNA
11. Introduction of asymmetry in the naturally symmetric restriction endonuclease EcoRV to investigate intersubunit communication in the homodimeric protein
12. FokI requires two specific DNA sites for cleavage
13. Structure of FokI has implications for DNA cleavage
14. Empirical aspects of strand displacement amplification
15. Strand displacement amplification- an isothermal, in vitro DNA amplification technique
16. Preparation of DNA substrates for in vitro mismatch repair
17. Restriction and modification systems