Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris

BioTechniques

Volumn 38, Issue 1, 2005, Pages 44-48

  Lin Cereghino, Joan ^a   Wong, William W ^a   Xiong, See ^a   Giang, William ^a   Luong, Linda T ^a   Vu, Jane ^a   Johnson, Sabrina D ^a   Lin Cereghino, Geoff P ^a  

^a UNIVERSITY OF THE PACIFIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DITHIOTHREITOL; FUNGAL DNA; PLASMID VECTOR;

ARTICLE; AUXOTROPHY; CELL HYBRIDIZATION; CELL TRANSFORMATION; CLONING VECTOR; CONTROLLED STUDY; ELECTROPORATION; FUNGAL STRAIN; FUNGUS CULTURE; GENE INSERTION; GENETIC MARKER; HEAT SHOCK; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION;

FUNGI; INSERTION SEQUENCES; PICHIA; PICHIA PASTORIS; UNIDENTIFIED CLONING VECTOR;

EID: 12344321400     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05381BM04     Document Type: Article

References (5)

1. Lin Cereghino, J. and J.M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24:45-66.
2. Cregg, J.M., K.J. Barringer, A.Y. Hessler, and K.R. Madden. 1985. Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5:3376-3385.
3. Cregg, J.M. and K.A. Russell. 1998. Transformation methods, p. 27-39. In D.R. Higgins and J.M. Cregg (Eds.), Methods in Molecular Methods Molecular Biology, Vol. 103: Pichia Protocols. Humana Press, Totowa, NJ.
4. Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
5. Dohmen, R.J., A.W.M. Strasser, C.B. Höner, and C.P. Hollenberg. 1991. An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera. Yeast 7:691-692.