Rapid expression screening of eukaryotic membrane proteins in Pichia pastoris

Protein Science

Volumn 22, Issue 4, 2013, Pages 425-433

  Brooks, Cory L ^a   Morrison, Melissa ^a   Lemieux, M Joanne ^a,b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b Canada Research in Membrane Protein Structure and Function   (Canada)

Author keywords

GFP; Membrane protein expression; Pichia pastoris; Structural genomics

Indexed keywords

AQUAPORIN 4; EUKARYOTIC MEMBRANE PROTEIN; MEMBRANE PROTEIN; PHOSPHATIDYLETHANOLAMINE METHYLTRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; MOLECULAR CLONING; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION;

ANIMALS; AQUAPORIN 4; BIOTECHNOLOGY; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GREEN FLUORESCENT PROTEINS; HUMANS; MEMBRANE PROTEINS; MICE; PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE; PICHIA; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS;

EUKARYOTA; PICHIA PASTORIS;

EID: 84878269745     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2223     Document Type: Article

References (36)

1. Liu J, Rost B (2001) Comparing function and structure between entire proteomes. Protein Sci 10:1970-1979. (Pubitemid 32911216)
2. Sanders CR, Myers JK (2004) Disease-related misassembly of membrane proteins. Annu Rev Biophys Biomol Struct 33:25-51. (Pubitemid 38829950)
3. Bill RM, Henderson PJ, Iwata S, Kunji ER, Michel H, Neutze R, Newstead S, Poolman B, Tate CG, Vogel H (2011) Overcoming barriers to membrane protein structure determination. Nat Biotechnol 29:335-340.
4. Korepanova A, Gao FP, Hua Y, Qin H, Nakamoto RK, Cross TA (2005) Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli. Protein Sci 14:148-158. (Pubitemid 40054126)
5. Kawate T, Michel JC, Birdsong WT, Gouaux E (2009) Crystal structure of the ATP-gated P2X(4) ion channel in the closed state. Nature 460:592-598.
6. Chen YH, Hu L, Punta M, Bruni R, Hillerich B, Kloss B, Rost B, Love J, Siegelbaum SA, Hendrickson WA (2010) Homologue structure of the SLAC1 anion channel for closing stomata in leaves. Nature 467: 1074-1080.
7. Hu J, Xue Y, Lee S, Ha Y (2011) The crystal structure of GXGD membrane protease FlaK. Nature 475: 528-531.
8. Cao Y, Jin X, Huang H, Derebe MG, Levin EJ, Kabaleeswaran V, Pan Y, Punta M, Love J, Weng J, Quick M, Ye S, Kloss B, Bruni R, Martinez-Hackert E, Hendrickson WA, Rost B, Javitch JA, Rajashankar KR, Jiang Y, Zhou M. (2011) Crystal structure of a potassium ion transporter, TrkH. Nature 471:336-340.
9. Hu J, Xue Y, Lee S, Ha Y (2011) The crystal structure of GXGD membrane protease FlaK. Nature 475: 528-U130.
10. Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC (1998) Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science 282:2220-2226. (Pubitemid 29004063)
11. Drew DE, von Heijne G, Nordlund P, de Gier JWL (2001) Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett 507:220-224. (Pubitemid 33001546)
12. Drew D, Slotboom DJ, Friso G, Reda T, Genevaux P, Rapp M, Meindl-Beinker NM, Lambert W, Lerch M, Daley DO, Van Wijk KJ, Hirst J, Kunji E, De Gier JW. (2005) A scalable, GFP-based pipeline for membrane protein overexpression screening and purification. Protein Sci 14:2011-2017. (Pubitemid 41132366)
13. Kawate T, Gouaux E (2006) Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure 14: 673-681.
14. Drew D, Newstead S, Sonoda Y, Kim H, von Heijne G, Iwata S (2008) GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nat Protoc 3:784-798. (Pubitemid 351639023)
15. Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D, Iwata S, Newstead S (2012) Alternating access mechanism in the POT family of oligopeptide transporters. EMBO J 31:3411-3421.
16. Hu NJ, Iwata S, Cameron AD, Drew D (2011) Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT. Nature 478:408-411.
17. Newstead S, Drew D, Cameron AD, Postis VL, Xia X, Fowler PW, Ingram JC, Carpenter EP, Sansom MS, McPherson MJ, Baldwin SA, Iwata S. (2011) Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2. EMBO J 30:417-426.
18. Hino T, Arakawa T, Iwanari H, Yurugi-Kobayashi T, Ikeda-Suno C, Nakada-Nakura Y, Kusano-Arai O, Weyand S, Shimamura T, Nomura N, Cameron AD, Kobayashi T, Hamakubo T, Iwata S, Murata T. (2012) G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody. Nature 482:237-240.
19. Shimamura T, Shiroishi M, Weyand S, Tsujimoto H, Winter G, Katritch V, Abagyan R, Cherezov V, Liu W, Han GW, Kobayashi T, Stevens RC, Iwata S. (2011) Structure of the human histamine H1 receptor complex with doxepin. Nature 475:65-70.
20. Miller AN, Long SB (2012) Crystal structure of the human two-pore domain potassium channel K2P1. Science 335:432-436.
21. Long SB, Campbell EB, Mackinnon R (2005) Crystal structure of a mammalian voltage-dependent Shaker family K+ channel. Science 309:897-903. (Pubitemid 41099919)
22. Tornroth-Horsefield S, Wang Y, Hedfalk K, Johanson U, Karlsson M, Tajkhorshid E, Neutze R, Kjellbom P (2006) Structural mechanism of plant aquaporin gating. Nature 439:688-694. (Pubitemid 43237610)
23. Ho JD, Yeh R, Sandstrom A, Chorny I, Harries WE, Robbins RA, Miercke LJ, Stroud RM (2009) Crystal structure of human aquaporin 4 at 1.8 A and its mechanism of conductance. Proc Natl Acad Sci USA 106: 7437-7442.
24. Aller SG, Yu J, Ward A, Weng Y, Chittaboina S, Zhuo R, Harrell PM, Trinh YT, Zhang Q, Urbatsch IL, Chang G. (2009) Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding. Science 323: 1718-1722.
25. Krogh A, Larsson B, von Heijne G, Sonnhammer ELL (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305:567-580. (Pubitemid 33032862)
26. Li Z, Vance DE (2008) Phosphatidylcholine and choline homeostasis. J Lipid Res 49:1187-1194.
27. Jacobs RL, Zhao Y, Koonen DP, Sletten T, Su B, Lingrell S, Cao G, Peake DA, Kuo MS, Proctor SD, Kennedy BP, Dyck JR, Vance DE. (2010) Impaired de novo choline synthesis explains why phosphatidylethanolamine N-methyltransferase- deficient mice are protected from diet-induced obesity. J Biol Chem 285: 22403-22413.
28. Cole LK, Dolinsky VW, Dyck JR, Vance DE (2011) Impaired phosphatidylcholine biosynthesis reduces atherosclerosis and prevents lipotoxic cardiac dysfunction in ApoE-/- mice. Circ Res 108:686-694.
29. Abramoff MD, Magalhaes PJ, Ram SJ (2004) Image processing with ImageJ. Biophoton Intl 11:36-42.
30. Lemieux MJ, Song J, Kim MJ, Huang Y, Villa A, Auer M, Li XD, Wang DN (2003) Three-dimensional crystallization of the Escherichia coli glycerol-3-phosphate transporter: a member of the major facilitator superfamily. Protein Sci 12:2748-2756. (Pubitemid 37445099)
31. Cornvik T, Dahlroth SL, Magnusdottir A, Herman MD, Knaust R, Ekberg M, Nordlund P (2005) Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli. Nat Methods 2:507-509.
32. Clare JJ, Rayment FB, Ballantine SP, Sreekrishna K, Romanos MA (1991) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Biotechnology 9:455-460.
33. Hohenblum H, Gasser B, Maurer M, Borth N, Mattanovich D (2004) Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol Bioeng 85:367-375. (Pubitemid 38186091)
34. Norden K, Agemark M, Danielson JA, Alexandersson E, Kjellbom P, Johanson U (2011) Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. BMC Biotechnol 11:47.
35. Guerfal M, Ryckaert S, Jacobs PP, Ameloot P, Van Craenenbroeck K, Derycke R, Callewaert N (2010) The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins. Microb Cell Fact 9:49.
36. Cormier CY, Mohr SE, Zuo D, Hu Y, Rolfs A, Kramer J, Taycher E, Kelley F, Fiacco M, Turnbull G, LaBaer J. (2010) Protein structure initiative material repository: an open shared public resource of structural genomics plasmids for the biological community. Nucleic Acids Res 38:D743-D749.