Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Krainer, Florian W ^a   Dietzsch, Christian ^b   Hajek, Tanja ^a   Herwig, Christoph ^b   Spadiut, Oliver ^b   Glieder, Anton ^c  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

^c AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

Author keywords

Candida antarctica lipase B; Dihydroxyacetone synthase; Formaldehyde dehydrogenase; Horseradish peroxidase; Methanol utilization pathway; Mut +; Mut S; Pichia pastoris; Recombinant protein expression; Transketolase

Indexed keywords

DIHYDROXYACETONE; FORMALDEHYDE DEHYDROGENASE; HORSERADISH PEROXIDASE; LIPASE B; METHANOL; RECOMBINANT PROTEIN; TRANSKETOLASE; ALDEHYDE DEHYDROGENASE; FORMALDEHYDE DEHYDROGENASE, GLUTATHIONE INDEPENDENT; FORMALDEHYDE DEHYDROGENASE, GLUTATHIONE-INDEPENDENT; FORMALDEHYDE TRANSKETOLASE; LIPASE B, CANDIDA ANTARCTICA; TRANSFERASE; TRIACYLGLYCEROL LIPASE;

ARTICLE; CANDIDA ANTARCTICA; CARBON SOURCE; CATALYSIS; FUNGAL STRAIN; NONHUMAN; PHENOTYPE; PICHIA PASTORIS; PROTEIN EXPRESSION; BIOSYNTHESIS; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PICHIA;

ARMORACIA RUSTICANA; CANDIDA ANTARCTICA; PICHIA PASTORIS;

ALDEHYDE OXIDOREDUCTASES; ALDEHYDE-KETONE TRANSFERASES; GENETIC ENGINEERING; HORSERADISH PEROXIDASE; LIPASE; METHANOL; PHENOTYPE; PICHIA; RECOMBINANT PROTEINS; TRANSKETOLASE;

EID: 84856790732     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-22     Document Type: Article

References (45)

1. Abad S, Nahalka J, Winkler M, Bergler G, Speight R, Glieder A, Nidetzky B. High-level expression of Rhodotorula gracilis d-amino acid oxidase in Pichia pastoris. Biotechnol Lett 2011, 33:557-563. 10.1007/s10529-010-0456-9, 21053050.
2. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. Fems Microbiol Rev 2000, 24:45-66. 10.1111/j.1574-6976.2000.tb00532.x, 10640598.
3. Cregg JM, Cereghino JL, Shi JY, Higgins DR. Recombinant protein expression in Pichia pastoris. Mol Biotechnol 2000, 16:23-52. 10.1385/MB:16:1:23, 11098467.
4. Cereghino GPL, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol 2002, 13:329-332. 10.1016/S0958-1669(02)00330-0, 12323354.
5. Hartner FS, Glieder A. Regulation of methanol utilisation pathway genes in yeasts. Microbial Cell Factories 2006, 5:39. 10.1186/1475-2859-5-39, 1781073, 17169150.
6. Couderc R, Baratti J. Oxidation of Methanol by the Yeast, Pichia-Pastoris - Purification and Properties of the Alcohol Oxidase. Agric Biol Chem 1980, 44:2279-2289.
7. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM. Heterologous protein production using the Pichia pastoris expression system. Yeast 2005, 22:249-270. 10.1002/yea.1208, 15704221.
8. Cregg JM, Madden KR, Barringer KJ, Thill GP, Stillman CA. Functional-Characterization of the 2 Alcohol Oxidase Genes from the Yeast Pichia-Pastoris. Mol Cel Biol 1989, 9:1316-1323.
9. Brierley RA, Bussineau C, Kosson R, Melton A, Siegel RS. Fermentation Development of Recombinant Pichia-Pastoris Expressing the Heterologous Gene - Bovine Lysozyme. Ann N Y Acad Scie 1990, 589:350-362.
10. Hartner FS, Ruth C, Langenegger D, Johnson SN, Hyka P, Lin-Cereghino GP, Lin-Cereghino J, Kovar K, Cregg JM, Glieder A. Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res 2008, 36:e76. 10.1093/nar/gkn369, 2475614, 18539608.
11. Cregg JM, Tschopp JF, Stillman C, Siegel R, Akong M, Craig WS, Buckholz RG, Madden KR, Kellaris PA, Davis GR, et al. High-Level Expression and Efficient Assembly of Hepatitis-B Surface-Antigen in the Methylotrophic Yeast, Pichia-Pastoris. Bio-Technology 1987, 5:479-485.
12. Romanos MA, Scorer CA, Clare JJ. Foreign Gene-Expression in Yeast - a Review. Yeast 1992, 8:423-488. 10.1002/yea.320080602, 1502852.
13. van den Burg HA, de Wit PJGM, Vervoort J. Efficient C-13/N-15 double labeling of the avirulence protein AVR4 in a methanol-utilizing strain (Mut(+)) of Pichia pastoris. J Biomol Nmr 2001, 20:251-261. 10.1023/A:1011206701288, 11519748.
14. Kim SJ, Lee JA, Kim YH, Song BK. Optimization of the Functional Expression of Coprinus cinereus Peroxidase in Pichia pastoris by Varying the Host and Promoter. J Microbiol Biotechnol 2009, 19:966-971. 10.4014/jmb.0901.018, 19809254.
15. Chiruvolu V, Cregg JM, Meagher MM. Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fedbatch fermentations. Enzyme and Microb Technol 1997, 21:277-283.
16. Kuberl A, Schneider J, Thallinger GG, Anderl I, Wibberg D, Hajek T, Jaenicke S, Brinkrolf K, Goesmann A, Szczepanowski R, et al. High-quality genome sequence of Pichia pastoris CBS7435. J Biotechnol 2011, 154:312-320. 10.1016/j.jbiotec.2011.04.014, 21575661.
17. Swartz JR, Cooney CL. Methanol Inhibition in Continuous Culture of Hansenula-Polymorpha. Appl Environ Microbiol 1981, 41:1206-1213. 243890, 16345771.
18. Hazeu W, Donker RA. A Continuous Culture Study of Methanol and Formate Utilization by the Yeast Pichia-Pastoris. Biotechnol Lett 1983, 5:399-404.
19. Kern A, Hartner FS, Freigassner M, Spielhofer J, Rumpf C, Leitner L, Frohlich KU, Glieder A. Pichia pastoris "just in time" alternative respiration. Microbiology 2007, 153:1250-1260. 10.1099/mic.0.2006/001404-0, 17379734.
20. Arnau C, Casas C, Valero F. The effect of glycerol mixed substrate on the heterologous production of a Rhizopus oryzae lipase in Pichia pastoris system. Biochem Eng J 2011, 57:30-37.
21. Jungo C, Marison I, von Stockar U. Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures. J Biotechnol 2007, 128:824-837. 10.1016/j.jbiotec.2006.12.024, 17303281.
22. Zhang WH, Potter KJH, Plantz BA, Schlegel VL, Smith LA, Meagher MM. Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement. J Ind Microbiol Biotechnol 2003, 30:210-215.
23. Orman MA, Calik P, Ozdamar TH. The influence of carbon sources on recombinant-human-growth-hormone production by Pichia pastoris is dependent on phenotype: a comparison of Mut(s) and Mut(+) strains. Biotechnol App Biochem 2009, 52:245-255.
24. Ascacio-Martinez JA, Barrera-Saldana HA. Production and secretion of biologically active recombinant canine growth hormone by Pichia pastoris. Gene 2004, 340:261-266. 10.1016/j.gene.2004.06.058, 15475167.
25. Pla IA, Damasceno LM, Vannelli T, Ritter G, Batt CA, Shuler ML. Evaluation of Mut(+) and Mut(S) Pichia pastoris phenotypes for high level extracellular scFv expression under feedback control of the methanol concentration. Biotechnol Prog 2006, 22:881-888. 10.1021/bp060012+, 16739975.
26. Lee B, Yurimoto H, Sakai Y, Kato N. Physiological role of the glutathione-dependent formaldehyde dehydrogenase in the methylotrophic yeast Candida boidinii. Microbiology-Sgm 2002, 148:2697-2704.
27. Nakagawa T, Ito T, Fujimura S, Chikui M, Mizumura T, Miyaji T, Yurimoto H, Kato N, Sakai Y, Tomizuka N. Molecular characterization of the glutathione-dependent formaldehyde dehydrogenase gene FLD1 from the methylotrophic yeast Pichia methanolica. Yeast 2004, 21:445-453. 10.1002/yea.1110, 15116345.
28. Schroer K, Luef KP, Hartner FS, Glieder A, Pscheidt B. Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation. Metab Eng 2010, 12:8-17. 10.1016/j.ymben.2009.08.006, 19716429.
29. Slekar KH, Kosman DJ, Culotta VC. The yeast copper zinc superoxide dismutase and the pentose phosphate pathway play overlapping roles in oxidative stress protection. J Biol Chem 1996, 271:28831-28836. 10.1074/jbc.271.46.28831, 8910528.
30. Dietzsch C, Spadiut O, Herwig C. A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris. Microbial Cell Factories 2011, 10:14. 10.1186/1475-2859-10-14, 3059269, 21371310.
31. Dietzsch C, Spadiut O, Herwig C. A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains. Microbial Cell Factories 2011, 10:85. 10.1186/1475-2859-10-85, 3214193, 22032177.
32. Morawski B, Lin ZL, Cirino PC, Joo H, Bandara G, Arnold FH. Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris. Protein Engineering 2000, 13:377-384. 10.1093/protein/13.5.377, 10835112.
33. Ohi H, Miura M, Hiramatsu R, Ohmura T. The Positive and Negative Cis-Acting Elements for Methanol Regulation in the Pichia-Pastoris Aox2 Gene. Mol Gen Genet 1994, 243:489-499. 10.1007/BF00284196, 8208240.
34. van der Heijden RTJM, Romein B, Heijnen JJ, Hellinga C, Luyben KCAM. Linear constraint relations in biochemical reaction systems: II. Diagnosis and estimation of gross errors. Biotechnol Bioeng 1994, 43:11-20. 10.1002/bit.260430104, 18613306.
35. Abad S, Nahalka J, Bergler G, Arnold SA, Speight R, Fotheringham I, Nidetzky B, Glieder A. Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst. Microbial Cell Factories 2010, 9:24. 10.1186/1475-2859-9-24, 2873405, 20420682.
36. Oka Y, Ishida H, Morioka M, Numasaki Y, Yamafuji T, Osono T, Umezawa H. Combimicins, New Kanamycin Derivatives Bioconverted by Some Micromonosporas. J Antibiot 1981, 34:777-781.
37. Wach A, Brachat A, Pohlmann R, Philippsen P. New Heterologous Modules for Classical or Pcr-Based Gene Disruptions in Saccharomyces-Cerevisiae. Yeast 1994, 10:1793-1808. 10.1002/yea.320101310, 7747518.
38. Lin-Cereghino J, Wong WW, Xiong S, Giang W, Luong LT, Vu J, Johnson SD, Lin-Cereghino GP. Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris. Biotechniques 2005, 38:44-48. 10.2144/05381BM04, 2504082, 15679083.
39. Weis R, Luiten R, Skranc W, Schwab H, Wubbolts M, Glieder A. Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena. Fems Yeast Research 2004, 5:179-189. 10.1016/j.femsyr.2004.06.016, 15489201.
40. Zhang NY, Suen WC, Windsor W, Xiao L, Madison V, Zaks A. Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution. Protein Engineering 2003, 16:599-605. 10.1093/protein/gzg074, 12968077.
41. Abad S, Kitz K, Hormann A, Schreiner U, Hartner FS, Glieder A. Real-time PCR-based determination of gene copy numbers in Pichia pastoris. Biotechnol J 2010, 5:413-420. 10.1002/biot.200900233, 20349461.
42. Hoffmann K, Onodera S, Noiva R. Expression of recombinant human protein disulfide isomerase in the yeast Pichia pastoris. Faseb J 1996, 10:677-677.
43. Marchler-Bauer A, Bryant SH. CD-Search: protein domain annotations on the fly. Nucleic Acids Res 2004, 32:W327-W331. 10.1093/nar/gkh454, 441592, 15215404.
44. Marchler-Bauer A, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR, Gwadz M, et al. CDD: specific functional annotation with the Conserved Domain Database. Nucleic Acids Resh 2009, 37:D205-D210.
45. Marchler-Bauer A, Lu SN, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR, et al. CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Res 2011, 39:D225-D229. 10.1093/nar/gkq1189, 3013737, 21109532.