Expression and secretion of recombinant ZZ-EGFP fusion protein by the methylotrophic yeast Pichia pastoris

Biotechnology Letters

Volumn 30, Issue 8, 2008, Pages 1409-1414

  Tang, Jin Bao ^a,b   Zhu, Peng ^b   Yang, Hong Ming ^a   Sun, Li Min ^b   Song, Shu Liang ^b   Ji, Ai Guo ^b  

^a WEIFANG MEDICAL UNIVERSITY   (China)

^b SHANDONG UNIVERSITY   (China)

Author keywords

Enhanced green fluorescent protein; Fusion protein; Immunofluorescence; Pichia pastoris; Secretion; ZZ peptide

Indexed keywords

ANTIBODIES; BOTTLES; FUSION REACTIONS; LIGHT EMISSION; LUMINESCENCE; METHANOL; NUCLEAR PHYSICS; YEAST;

BINDING CAPACITIES; BUSINESS MEDIA; ENHANCED GREEN FLUORESCENT PROTEIN (EGFP); FLUORESCEIN ISOTHIOCYANATE (FTIC); FUSION PROTEINS; HEXAHISTIDINE TAG; HUMAN IGG; IMMUNOFLUORESCENCE ASSAY (IFA); IMMUNOGLOBULIN G (IGG); METHYLOTROPHIC YEASTS; PICHIA PASTORIS (PPPFK); RECOMBINANT YEAST CELLS; SPRINGER (CO); STAPHYLOCOCCAL PROTEIN;

FLUORESCENCE;

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; IMMUNOGLOBULIN G; STAPHYLOCOCCUS PROTEIN A;

ARTICLE; CELL LINE; CHEMISTRY; FLUORESCENT ANTIBODY TECHNIQUE; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PICHIA; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN TERTIARY STRUCTURE; SECRETION;

CELL LINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; IMMUNOGLOBULIN G; PICHIA; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCAL PROTEIN A;

ANIMALS; ANTIBODIES; BOTTLES; FLUORESCENCE; LUMINESCENCE; METHANOL; NUCLEAR PHYSICS; PEPTIDES; PROTEINS; YEASTS;

ANIMALIA; PICHIA PASTORIS; STAPHYLOCOCCUS;

EID: 45849086556     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9714-5     Document Type: Article

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