Efficient vectors for expression cloning of large numbers of PCR fragments in P. pastoris

Yeast

Volumn 27, Issue 5, 2010, Pages 285-292

  Rao, Ben ^a   Zhong, Xing ^a   Wang, Yaping ^a   Wu, Qiong ^a   Jiang, Zhengbing ^a   Ma, Lixin ^a  

^a HUBEI UNIVERSITY   (China)

Author keywords

Blue white screening method; Immediate protein expression; LacO reconstruction; Pichia pastoris

Indexed keywords

BETA GALACTOSIDASE; DNA FRAGMENT; FUNGAL PROTEIN; PROTEIN BFUI; PROTEIN LACI; PROTEIN LACO; PROTEIN SALI; PROTEIN SCHI; PROTEIN YEV; PROTEIN YEVB; RECOMBINANT PROTEIN; RESTRICTION ENDONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME PURIFICATION; EXPRESSION VECTOR; GENE TARGETING; GENETIC COMPLEMENTATION; GENETIC TRANSFORMATION; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PICHIA PASTORIS; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING TEST;

CLONING, MOLECULAR; DNA RESTRICTION ENZYMES; GENE EXPRESSION; GENETIC VECTORS; LAC OPERON; OPEN READING FRAMES; PICHIA; PLASMIDS; POLYMERASE CHAIN REACTION; TRANSFORMATION, GENETIC;

MICROMESISTIUS POUTASSOU; PICHIA PASTORIS;

EID: 77952255621     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1753     Document Type: Article

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