Production of bifunctional single-chain antibody-based fusion proteins in Pichia pastoris supernatants

Bioprocess and Biosystems Engineering

Volumn 31, Issue 6, 2008, Pages 559-568

  Panjideh, Hossein ^a   Coelho, Vânia ^a   Dernedde, Jens ^a   Fuchs, Hendrik ^a   Keilholz, Ulrich ^a   Thiel, Eckhard ^a   Deckert, P Markus ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Enzyme; Fermentation; Fluorophore; Pichia pastoris; Purification; Recombinant fusion protein; Single chain

Indexed keywords

ENZYME; FLUOROPHORE; PICHIA PASTORIS; RECOMBINANT FUSION PROTEIN; SINGLE-CHAIN;

ANTIBODIES; BIOCHEMICAL ENGINEERING; CHEMICAL REACTORS; ENZYMES; FERMENTATION; FLOW CYTOMETRY; FLUORESCENCE; FLUOROPHORES; MAMMALS; PROTEINS;

PURIFICATION;

CYTOSINE DEAMINASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; ANTIBODY; ENZYME; METHANOL; OXYGEN; RECOMBINANT PROTEIN;

ARTICLE; BIOREACTOR; CONTROLLED STUDY; CYTOTOXICITY; FERMENTATION; GENE EXPRESSION SYSTEM; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SUPERNATANT; BIOMASS; CHEMISTRY; DOSE RESPONSE; FLOW CYTOMETRY; METABOLISM; METHODOLOGY; PH; PICHIA;

MAMMALIA; PICHIA; PICHIA PASTORIS;

ANTIBODIES; BIOMASS; BIOREACTORS; CYTOSINE DEAMINASE; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYMES; FERMENTATION; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HYDROGEN-ION CONCENTRATION; METHANOL; OXYGEN; PICHIA; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS;

EID: 55949107078     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-008-0203-y     Document Type: Article

References (34)

1. GP Adams LM Weiner 2005 Monoclonal antibody therapy of cancer Nat Biotechnol 23 1147 1157
2. LM Damasceno KA Anderson G Ritter JM Cregg LJ Old CA Batt 2006 Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris Appl Microbiol Biotechnol 74 381 389
3. H Dorai JE [tmp] McCartney RM Hudziak MS Tai AA Laminet LL Houston JS Huston H Oppermann 1994 Mammalian cell expression of single-chain Fv (sFv) antibody proteins and their C-terminal fusions with interleukin-2 and other effector domains Biotechnology (N Y) 12 890 897
4. J Suzuki M Fukuda S Kawata M Maruoka Y Kubo T Takeya T Shishido 2006 A rapid protein expression and purification system using Chinese hamster ovary cells expressing retrovirus receptor J Biotechnol 126 463 474
5. R Ridder R Schmitz F Legay H Gram 1995 Generation of rabbit monoclonal antibody fragments from a combinatorial phage display library and their production in the yeast Pichia pastoris Biotechnology (N Y) 13 255 260
6. FM Freyre JE Vazquez M Ayala L Canaan-Haden H Bell I Rodriguez A Gonzalez A Cintado JV Gavilondo 2000 Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris J Biotechnol 76 157 163
7. C Marty P Scheidegger K Ballmer-Hofer R Klemenz RA Schwendener 2001 Production of functionalized single-chain Fv antibody fragments binding to the ED-B domain of the B-isoform of fibronectin in Pichia pastoris Protein Expr Purif 21 156 164
8. C Gurkan DJ Ellar 2005 Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris Microb Cell Fact 4 33
9. J Liu D Wei F Qian Y Zhou J Wang Y Ma Z Han 2003 pPIC9-Fc: a vector system for the production of single-chain Fv-Fc fusions in Pichia pastoris as detection reagents in vitro J Biochem (Tokyo) 134 911 917
10. LM Emberson AJ Trivett PJ Blower PJ Nicholls 2005 Expression of an anti-CD33 single-chain antibody by Pichia pastoris J Immunol Methods 305 135 151
11. KF Medzihradszky DI Spencer SK Sharma J Bhatia RB Pedley DA Read RH Begent KA Chester 2004 Glycoforms obtained by expression in Pichia pastoris improve cancer targeting potential of a recombinant antibody-enzyme fusion protein Glycobiology 14 27 37
12. H Kogelberg B Tolner SK Sharma MW Lowdell U Qureshi M Robson T Hillyer RB Pedley W Vervecken R Contreras RH Begent KA Chester 2007 Clearance mechanism of a mannosylated antibody-enzyme fusion protein used in experimental cancer therapy Glycobiology 17 36 45
13. Cregg J, Higgins DR (1998) Pichia Protocols. The Humana Press, Totowa
14. B Prinz J Schultchen R Rydzewski C Holz M Boettner U Stahl C Lang 2004 Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics J Struct Funct Genomics 5 29 44
15. B Tolner L Smith RH Begent KA Chester 2006 Production of recombinant protein in Pichia pastoris by fermentation Nat Protoc 1 1006 1021
16. SB Ellis PF Brust PJ Koutz AF Waters MM Harpold TR Gingeras 1985 Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris Mol Cell Biol 5 1111 1121
17. P Koutz GR Davis C Stillman K Barringer J Cregg G Thill 1989 Structural comparison of the Pichia pastoris alcohol oxidase genes Yeast 5 167 177
18. JD Chung 2000 Design of metabolic feed controllers: application to high-density fermentations of Pichia pastoris Biotechnol Bioeng 68 298 307
19. W Zhang MA Bevins BA Plantz LA Smith MM Meagher 2000 Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A Biotechnol Bioeng 70 1 8
20. MM Guarna HC Cote EA Amandoron RT [tmp] MacGillivray RA Warren DG Kilburn 1996 Engineering factor X fusions for expression in Pichia pastoris Ann N Y Acad Sci 799 397 400
21. JM Cregg JL Cereghino J Shi DR Higgins 2000 Recombinant protein expression in Pichia pastoris Mol Biotechnol 16 23 52
22. B Prinz U Stahl C Lang 2003 Intracellular transport of a heterologous membrane protein, the human transferrin receptor, in Saccharomyces cerevisiae Int Microbiol 6 49 55
23. LM Damasceno I Pla HJ Chang L Cohen G Ritter LJ Old CA Batt 2004 An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris Protein Expr Purif 37 18 26
24. Petrausch U, Dernedde J, Casimiro da Silva Coelho V, Panjideh H, Frey D, Fuchs H, Thiel E, Deckert PM. A33scFv::Green fluorescent protein, a recombinant single-chain fusion protein for tumor targeting. Prot Eng Des Sel; 2 Nov. 2007 [Epub ahead of print]
25. V Coelho J Dernedde U Petrausch H Panjideh H Fuchs C Menzel S Dübel PM Deckert 2007 Design, construction, and in vitro analysis of A33scFv::CDy, a recombinant fusion protein for antibody-directed enzyme prodrug therapy (ADEPT) in colon cancer Int.J.Oncol. 31 951 957
26. JM Cregg TS Vedvick WC Raschke 1993 Recent advances in the expression of foreign genes in Pichia pastoris Biotechnology (N Y) 11 905 910
27. C Rader G Ritter S Nathan M Elia I Gout AA Jungbluth LS Cohen S Welt LJ Old CF Barbas III 2000 The rabbit antibody repertoire as a novel source for the generation of therapeutic human antibodies J Biol Chem 275 13668 13676
28. S Macauley-Patrick ML Fazenda B [tmp] McNeil LM Harvey 2005 Heterologous protein production using the Pichia pastoris expression system Yeast 22 249 270
29. R Daly MT Hearn 2005 Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production J Mol Recognit 18 119 138
30. NA Gomes Pereira MA Juliano AK Carmona ED Sturrock GJ Kotwal 2005 Cloning and Expression of a Functionally Active Truncated N-Glycosylated KSHV ORF4/KCP/Kaposica in the Methylotrophic Yeast Pichia pastoris Ann N Y Acad Sci 1056 388 404
31. J Shi ST Zhang XJ Zhang H Xu AG Guo 2007 Expression, purification, and activity identification of Alfimeprase in Pichia pastoris Protein Expr Purif 54 240 246
32. MW Werten TJ van den Bosch RD Wind H Mooibroek FA de Wolf 1999 High-yield secretion of recombinant gelatins by Pichia pastoris Yeast 15 1087 1096
33. IA Pla LM Damasceno T Vannelli G Ritter CA Batt ML Shuler 2006 Evaluation of Mut+ and MutS Pichia pastoris phenotypes for high level extracellular scFv expression under feedback control of the methanol concentration Biotechnol Prog 22 881 888
34. PM Deckert C Renner LS Cohen A Jungbluth G Ritter JR Bertino LJ Old S Welt 2003 A33scFv-cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy Br J Cancer 88 937 939