Impact of Using Different Promoters and Matrix Attachment Regions on Recombinant Protein Expression Level and Stability in Stably Transfected CHO Cells

Molecular Biotechnology

Volumn 57, Issue 2, 2015, Pages 138-144

  Ho, Steven C L ^a   Mariati, ^a   Yeo, Jessna H M ^a   Fang, Shiyi Goh ^a   Yang, Yuansheng ^a,b  

^a AGENCY FOR SCIENCE   (Singapore)

^b Nanyang Technological University   (Singapore)

Author keywords

CHO cells; CHO elongation factor 1 promoter; CMV promoter; Matrix attachment region (MAR); Stable recombinant protein expression; SV40 promoter

Indexed keywords

CELL CULTURE; GENE EXPRESSION; MAMMALS; STABILITY; TOBACCO; VIRUSES;

CHO CELL; CMV PROMOTER; ELONGATION FACTOR; MATRIX ATTACHMENT REGION; RECOMBINANT PROTEIN EXPRESSION; SV40 PROMOTER;

RECOMBINANT PROTEINS;

BETA INTERFERON; ELONGATION FACTOR 1ALPHA; LYSOZYME; MESSENGER RNA; PLASMID VECTOR; RECOMBINANT PROTEIN;

ARTICLE; CELL CLONE; CHO CELL LINE; CLINICAL EFFECTIVENESS; COMPARATIVE STUDY; CONTROLLED STUDY; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CYTOMEGALOVIRUS; IMMEDIATE EARLY GENE; MAMMAL CELL; MATRIX ATTACHMENT REGION; NONHUMAN; PROMOTER REGION; PROTEIN STABILITY; RECOMBINANT GENE; SIMIAN VIRUS 40; STABLE EXPRESSION; TRANSGENE; ANIMAL; BIOSYNTHESIS; CRICETULUS; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; HAMSTER;

HUMAN HERPESVIRUS 5; MAMMALIA; SIMIAN VIRUS 40;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; GENE EXPRESSION REGULATION; GENETIC VECTORS; HUMANS; MATRIX ATTACHMENT REGIONS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; RNA, MESSENGER; SIMIAN VIRUS 40; TRANSFECTION;

EID: 84921385817     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-014-9809-2     Document Type: Article

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