Human beta interferon scaffold attachment region inhibits de novo methylation and confers long-term, copy number-dependent expression to a retroviral vector

Journal of Virology

Volumn 74, Issue 6, 2000, Pages 2671-2678

  Dang, Qi ^a   Auten, Jennifer ^a   Plavec, Ivan ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BETA INTERFERON; DNA; VIRUS VECTOR;

ARTICLE; CONTROLLED STUDY; DNA METHYLATION; DNA SEQUENCE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LONG TERMINAL REPEAT; METHYLATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; RETROVIRUS;

ANIMALS; BINDING SITES; CELL LINE; CELL LINE, TRANSFORMED; DNA METHYLATION; GENE EXPRESSION REGULATION, VIRAL; GENETIC VECTORS; HUMANS; INTERFERON-BETA; MICE; MOLONEY MURINE LEUKEMIA VIRUS; PROVIRUSES; T-LYMPHOCYTES; TERMINAL REPEAT SEQUENCES; TIME FACTORS; TRANSFECTION;

EID: 0034088115     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.6.2671-2678.2000     Document Type: Article

References (43)

1. Agarwal, M., T. W. Austin, F. Morel, J. Chen, E. Böhnlein, and I. Plavec. 1998. Scaffold attachment region-mediated enhancement of retroviral vector expression in primary T cells. J. Virol. 72:3720-3728.
2. Akgun, E., M. Ziegler, and M. Grez. 1991. Determinants of retrovirus gene expression in embryonal carcinoma cells. J. Virol. 65:382-388.
3. Auten, J., M. Agarwal, J. Chen, R. Sutton, and I. Plavec. 1999. Effect of scaffold attachment region on transgene expression in retrovirus vector transduced primary T cells and macrophages. Hum. Gene Ther. 10:1389-1399.
4. Baum, C., S. Hegewisch-Becker, H. G. Eckert, C. Stocking, and W. Ostertag. 1995. Novel retroviral vectors for efficient expression of the multidrug resistance (mdr-1) gene in early hematopoietic cells. J. Virol. 69:7541-7547.
5. Bode, J., T. Schlake, M. Ríos-Ramírez, C. Mielke, M. Stengert, V. Kay, and D. Klehr-Wirth. 1995. Scaffold/matrix-attachment regions (S/MAR): structural properties creating transcriptionally active loci. Academic Press, Inc., Orlando. Fla.
6. Boulikas, T. 1993. Nature of DNA sequences at the attachment regions of genes to the nuclear matrix. J. Cell. Biochem. 52:14-22.
7. Challita, P. M., and D. B. Kohn. 1994. Lack of expression from a retroviral vector after transduction of murine hematopoietic stem cells is associated with methylation in vivo. Proc. Natl. Acad. Sci. USA 91:2567-2571.
8. Challita, P. M., D. Skelton, A. El-Khoueiry, X. J. Yu, K. Weinberg, and D. B. Kohn. 1995. Multiple modifications in cis elements of the long terminal repeat of rctroviral vectors lead to increased expression and decreased DNA methylation in embryonic carcinoma cells. J. Virol. 69:748-755.
9. Cockerill, P. N., and W. T. Garrard. 1986. Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites. Cell 44:273-282.
10. Comb, M., and H. M. Goodman. 1990. CpG methylation inhibits proenkephalin gene expression and binding of the transcription factor AP-2. Nucleic Acids Res. 18:3975-3982.
11. Duch, M., K. Paludan, P. Jørgensen, and F. S. Pedersen. 1994. Lack of correlation between basal expression levels and susceptibility to transcriptional shutdown among single-gene murine leukemia virus vector proviruses. J. Virol. 68:5596-5601.
12. Duch, M., K. Paludan, J. Lovmand, L. Pedersen, P. Jøorgensen, and F. S. Pedersen. 1993. A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses. Nucleic Acids Res. 21:4777-4782.
13. Forestell, S. P., E. Böhnlein, and R. J. Rigg. 1995. Retroviral end-point titer is not predictive of gene transfer efficiency: implications for vector production. Gene Ther. 2:723-730
14. Gasser, S. M., and U. K. Laemmli. 1986. Cohabitation of scaffold binding regions with upstream enhancer elements of three developmentally regulated genes of D. melanogaster. Cell 46:521-530.
15. Gorman, C. M., and P. W. Rigby, 1985. Negative regulation of viral enhancers in undifferentiated embryonic stem cells. Cell 42:519-526.
16. Haapala, D. K., S. D. Robay, S. D. Oroszlan, and W. P. Tsai. 1985. Isolation from cats of an endogenous type C virus with a novel glycoprotein. J. Virol. 53:827-833.
17. Harbers, K., A. Schnieke, H. Stuhlman, D. Jahner, and R. Jaenisch. 1981. DNA methylation and gene expression: endogenous retroviral genome becomes infectious after molecular cloning. Proc. Natl. Acad. Sci. USA 78: 7609-7613.
18. Hawley, R. G., F. H. L. Lieu, A. Z. C. Fong, and T. S. Hawley. 1994. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1:136-138.
19. Hoeben, R. C., A. A. Migchielsen, R. C. van der Jagt, H. van Ormondt, and A. J. van der Eb. 1991. Inactivation of the Moloney murine leukemia virus long terminal repeat in murine fibroblast cell lines is associated with methylation and dependent on its chromosomal position. J. Virol. 65:904-912.
20. Hsieh, C. L. 1994. Dependence of transcriptional repression on CpG methylation density. Mol. Cell. Biol. 14:5487-5494.
21. Iguchi-Ariga, S. M., and W. Schaffner. 1989. CpG methylation of the cAMP-responsive enhancer/promoter sequence TGACGTCA abolishes specific factor binding as well as transcriptional activation. Genes Dev. 3:612-619.
22. Jenuwein. T., W. C. Forrester, L. A. Fernández-Herrero, G. Laibe, M. Dull, and R. Grosschedl. 1997. Extension of chromatin accessibility by nuclear matrix attachment regions. Nature 385:269-272.
23. Jones, P. L., G. J. Veenstra, P. A. Wade, D. Vermaak, S. U. Kass, N. Landsberger, J. Strouboulis, and A. P. Wolffe. 1998. Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription. Nat. Genet. 19:187-191.
24. Kass, S. U., D. Pruss, and A. P. Wolffe. 1997. How does DNA methylation repress transcription? Trends Genet. 13:444-449.
25. Kirillov, A., B. Kistler, R. Mostoslavsky, H. Ceder, T. Wirth, and Y. Bergman. 1996. A role for nuclear NF-kappaB in B-cell-specific demethylation of the 1g kappa locus. Nat. Genet. 13:435-441.
26. Lichtenstein, M., G. Keini, H. Cedar, and Y. Bergman. 1994. B cell-specific demethylation: a novel role for the intronic kappa chain enhancer sequence. Cell 76:913-923.
27. McKnight, R. A., A. Shamay, L. Sankaran, R.J. Wall, and L. Hennighausen. 1992. Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice. Proc. Natl. Acad. Sci. USA 89:6943-6947.
28. Mulligan, R. C. 1993. The basic science of gene therapy. Science 260:926-932.
29. Nan, X., H. H. Ng, C. A. Johnson, C. D. Laherty, B. M. Turner, R. N. Eisenman, and A. Bird. 1998. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature 393:386-389.
30. Niwa, O., Y. Yokota, H. Ishida, and T. Sugahara. 1983. Independent mechanisms involved in suppression of the Moloney murine leukemia virus genome during differentiation of murine teratocarcinoma cells. Cell 32:1105-1113.
31. Palmer, T. D., G. J. Rosman, W. R. Osborne, and A. D. Miller. 1991. Genetically modified skin fibroblasts persist long after transplantation but gradually inactivate introduced genes. Proc. Natl. Acad. Sci. USA 88:1330-1334.
32. Pawliuk, R., C. J. Eaves, and R. K. Humphries. 1997. Sustained high-level reconstitution of the hematopoietic system by preselected hematopoietic cells expressing a transduced cell-surface antigen. Hum. Gene Ther. 8:1595-1604.
33. Phi-Van, L., J. P. von Kries, W. Ostertag, and W. H. Stratling. 1990. The chicken lysozyme 5́ matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes. Mol. Cell. Biol. 10:2302-2307.
34. Plavec, I., and M. Agarwal. Utility of scaffold attachment regions for enhanced retroviral vector expression in human hematopoietic cells. In A. C.-A. A. Garcia (ed.), Viral vectors: basic science and gene therapy, in press. Eaton Publishing, Natick, Mass.
35. Rigs, J. R., J. Chen, J. S. Dando, S. P. Forestell, I. Plavec, and E. Böhnlein. 1996. A novel human amphotropic packaging cell line: high titer, complement resistance, and improved safety. Virology 218:290-295.
36. Rivière, I., K. Brose, and R. C. Mulligan. 1995. Effect of retroviral vector design on expression of human adenosine deaminase in murine bone marrow transplant recipients engrafted with genetically modified cells. Proc. Natl. Acad. Sci. USA 92:6733-6737.
37. Robbins, P. B., D. C. Skelton, X. J. Yu, S. Halene, E. H. Leonard, and D. B. Kuhn. 1998. Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells. Proc. Natl. Acad. Sci. USA 95: 10182-10187.
38. Singal, R., R. Ferris, J. A. Little, S. Z. Wang, and G. D. Ginder. 1997. Methylation of the minimal promoter of an embryonic globin gene silences transcription in primary erythroid cells. Proc. Natl. Acad. Sci. USA 94: 13724-13729.
39. Singal, R., and G. D. Ginder. 1999. DNA methylation. Blood 93:4059-4070.
40. Stewart, C. L., H. Stuhlman, D. Jahner, and R. Jaenisch. 1982. De novo methylation, expression, and infectivity of retroviral genomes introduced into embryonal carcinoma cells. Proc. Natl. Acad. Sci. USA 79:4098-4102.
41. Thompson, E. M., E. Christians, M.-G. Stinnakre, and J.-P. Renard. 1994. Scaffold attachment regions stimulate HSP70.1 expression in mouse preimplantation embryos but not in differentiated tissues. Mol. Cell. Biol. 14:4694-4703.
42. Tsukiyama, T., H. Ueda, S. Hirose, and O. Niwa. 1992. Embryonal long terminal repeat-binding protein is a murine homolog of FTZ-F1, a member of the steroid receptor superfamily. Mol. Cell. Biol. 12:1286-1291.
43. Wang, L., P. B. Rabbins, D. A. Carbonaro, and D. B. Kohn. 1998. High-resolution analysis of cytosine methylation in the 5′ long terminal repeat of retroviral vectors. Hum. Gene Ther. 9:2321-2330.