Enhanced expression of transgene in CHO cells using matrix attachment region

Cell Biology International

Volumn 32, Issue 10, 2008, Pages 1279-1283

  Wang, Tian Yun ^a   Yang, Rui ^b   Qin, Chuan ^b   Wang, Li ^a   Yang, Xian Jun ^b  

^a Department of Biochemistry and Molecular Biology ^*  (China)

^b XINXIANG MEDICAL UNIVERSITY   (China)

Author keywords

Gene expression; Gene silencing; Mammalian expression system; Matrix attachment region

Indexed keywords

BETA GLOBIN; CHLORAMPHENICOL ACETYLTRANSFERASE;

ANIMAL CELL; ARTICLE; CHO CELL; EXTRACELLULAR MATRIX; FEMALE; GENE EXPRESSION; GENETIC TRANSFECTION; NONHUMAN; PROMOTER REGION; TRANSGENE;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; GENE DOSAGE; GENE EXPRESSION REGULATION; GENES, REPORTER; GENETIC VECTORS; HUMANS; TRANSFECTION; TRANSGENES;

CRICETULUS GRISEUS; MAMMALIA;

EID: 52949141465     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cellbi.2008.07.014     Document Type: Article

References (20)

1. Bonifer C., Yannoutsos N., Krüger G., Grosveld F., and Sippel A.E. Dissection of the locus control function located on the chicken lysozyme gene domain in transgenic mice. Nucleic Acids Res 22 (1994) 4202-4210
2. Feng Y.Q., Lorincz M.C., Fiering S., Greally J.M., and Bouhassira E.E. Position effects are influenced by the orientation of a transgene with respect to flanking chromatin. Mol Cell Biol l21 (2001) 298-309
3. Girod P.A., Zahn-Zabal M., and Mermod N. Use of the chicken lysozyme 5′ matrix attachment region to generate high producer CHO cell lines. Biotechnol Bioeng 91 (2005) 1-11
4. Huber M.C., Bosch F.X., Sippel A.E., and Bonifer C. Chromosomal position effects in chicken lysozyme gene transgenic mice are correlated with suppression of DNase I hypersensitive site formation. Nucleic Acids Res 22 (1994) 4195-4201
5. Kalos M., and Fournier R.E. Position-independent transgene expression mediated by boundary elements from the apolipoprotein B chromatin domain. Mol Cell Biol 15 (1995) 198-207
6. Kaufman R.J. Overview of vector design for mammalian gene expression. Mol Biotechnol 16 (2000) 151-160
7. Kim J.M., Kim J.S., Park D.H., and Kang H.S. Improved recombinant gene expression in CHO cells using matrix attachment regions. J Biotechnol 107 (2004) 95-105
8. Lucas B.K., Giere L.M., DeMarco R.A., Shen A., Chisholm V., and Crowley C.W. High-level production of recombinant proteins in CHO cells using a dicistronic DHFR intron expression vector. Nucleic Acids Res 24 (1996) 1774-1779
9. McKnight R.A., Shamay A., Sankaran L., Wall R.J., and Hennighausen L. Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice. Proc Natl Acad Sci U S A 89 (1992) 6943-6947
10. McKnight R.A., Spencer M., Wall R.J., and Hennighausen L. Severe position effects imposed on a 1 kb mouse whey acidic protein gene promoter are overcome by heterologous matrix attachment regions. Mol Reprod Dev 44 (1996) 179-184
11. Namciu S.J., Blochlinger K.B., and Fournier R.E. Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster. Mol Cell Biol 18 (1998) 2382-2391
12. Poljak L., Seum C., Mattioni T., and Laemmli U.K. SARs stimulate but do not confer position independent gene expression. Nucleic Acids Res 22 (1994) 4386-4394
13. Phi-Van L., von Kries J.P., Ostertag W., and Stratling W.H. The chicken lysozyme 5′ matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes. Mol Cell Biol 10 (1990) 2302-2307
14. Spiker S., and Thompson W.F. Nuclear matrix attachment regions and transgene expression in plants. Plant Physiol 110 (1996) 15-21
15. Stief A., Winter D., Stratking W., and Sippel A. A nuclear DNA attachment element mediates elevated and position-independent gene activity. Nature 341 (1989) 343-345
16. Wang T., Xue L., Hou W., Yang B., Chai Y., Ji X., et al. Increased expression of transgene in stably transformed cells of Dunaliella salina by matrix attachment regions. Appl Microbiol Biotechnol 76 (2007) 651-657
17. Wang D.M., Taylor S., and Levy-Wilson B. Evaluation of the function of the human apolipoprotein B gene nuclear matrix association regions in transgenic mice. J Lipid Res 37 (1996) 2117-2124
18. Weikert S., Papac D., Briggs J., Cowfer D., Tom S., Gawlitzek M., et al. Engineering Chinese hamster ovary cells to maximize sialic acid content of recombinant glycoproteins. Nat Biotechnol 17 (1999) 1116-1121
19. Yu J., Bock J.H., Slightom J.L., and Villeponteau B. A 5′ beta-globin matrix-attachment region and the polyoma enhancer together confer position-independent transcription. Gene 139 (1994) 139-145
20. Zahn-Zabal M., Kobr M., Girod P.A., Imhof M., Chatellard P., de Jesus M., et al. Development of stable cell lines for production or regulated expression using matrix attachment regions. J Biotechnol 87 (2001) 29-42