Limitations to the development of humanized antibody producing chinese hamster ovary cells using glutamine synthetase-mediated gene amplification

Biotechnology Progress

Volumn 22, Issue 3, 2006, Pages 770-780

  Jun, Seung Chul ^a   Kim, Min Soo ^a   Hong, Hyo Jeong ^b   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; CELL CULTURE; CHROMOSOMES; CLONING; ENZYMES;

ANTIBODY EXPRESSION; CHINESE HAMSTER OVARY (CHO); FLUORESCENCE IN SITU HYBRIDIZATION (FISH); GENE AMPLIFICATION;

GENETIC ENGINEERING;

GLUTAMATE AMMONIA LIGASE; METHIONINE SULFOXIMINE; MONOCLONAL ANTIBODY;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CHO CELL; CULTURE MEDIUM; CULTURE TECHNIQUE; DOSE RESPONSE; DRUG EFFECT; GENE AMPLIFICATION; GENE VECTOR; GENETICS; HAMSTER; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR CLONING; NUCLEIC ACID HYBRIDIZATION; SOUTHERN BLOTTING; TIME;

ANIMALS; ANTIBODIES, MONOCLONAL; BLOTTING, SOUTHERN; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CULTURE MEDIA; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE AMPLIFICATION; GENETIC VECTORS; GLUTAMATE-AMMONIA LIGASE; HUMANS; METHIONINE SULFOXIMINE; NUCLEIC ACID HYBRIDIZATION; TIME FACTORS;

CRICETULUS GRISEUS;

EID: 33745045191     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp060004t     Document Type: Article

References (48)

1. Flintoff, W. F.; Essani, K. Methotrexate-resistant Chinese hamster ovary cells contain a dihydrofolate reductase with an altered affinity for methotrexate. Biochemistry 1980, 19, 4321-4327.
2. Assaraf, Y. G.; Schimke, R. T. Identification of methotrexate transport deficiency in mammalian cells using fluoresceinated methotrexate and flow cytometry. Proc. Natl. Acad. Sci. U.S.A. 1987, 84, 7154-7158.
3. Crouse, G. F.; McEwan, R. N.; Pearson, M. L. Expression and amplification of engineered mouse dihydrofolate reductase minigenes. Mol. Cell. Biol. 1983, 3, 257-266.
4. Subramani, S.; Mulligan, R.; Berg, P. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1981, 1, 854-864.
5. Cockett, M. I.; Bebbington, C. R.; Yarranton, G. T. High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification. Bio/Technology 1990, 8, 662-667.
6. Brown, M. E.; Renner, G.; Field, R. P.; Hassell, T. Process development for the production of recombinant antibodies using the glutamine synthetase (GS) system. Cytotechnology 1992, 9, 231-236.
7. Blochberger, T. C.; Cooper, C.; Peretz, D.; Tatzeit, J.; Griffith, O. H.; Baldwin, M. A.; Prusiner, S. B. Prion protein expression in Chinese hamster ovary cells using a glutamine synthetase selection and amplification system. Protein Eng. 1997, 10, 1465-1473.
8. Bebbington, C. R.; Renner, G.; Thomson, S.; King, D.; Abrams, D.; Yarranton, G. T. High-level expression of a recombinant antibody from myeloma cells using a glutamine-synthetase gene as an amplifiable selectable marker. Bio/Technology 1992, 10, 169-175.
9. Bibila, T.; Ranucci, C.; Glazomitsky, K.; Buckland, B.; Aunins, J. Investigation of NSO cell metabolic behavior in monoclonal antibody producing clones. Ann. N.Y. Acad. Sci. 1994, 745, 277-284.
10. Hassell, T.; Brand, H.; Renner, G.; Westlake, A.; Field, R. P. Stability of production of recombinant antibodies from glutamine synthetase amplified CHO and NS0 cell lines. In Animal Cell Technology: Development, Processes and Products; Spier, R. E., Griffiths, J. B., McDonald, C., Eds.; Butterworth-Heinemann: Oxford, 1992; pp 42-47.
11. Kim, S. J.; Kim, N. S.; Ryu, C. J.; Hong, H. J.; Lee, G. M. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 1998, 58, 73-84.
12. Lee, G. M.; Palsson, B. O. Immobilization can improve the stability of Hybridoma antibody productivity in serum-free media. Biotechnol. Bioeng. 1990, 36, 1049-1055.
13. Ausubel, F. M.; Brent, R.; Kingston, R. E.; Moore, D. D.; Seidman, J. G.; Smith, J. A.; Struhl, K. Short Protocols in Molecular Biology; John Wiley & Sons: New York, 1999; pp 2-39-2-42.
14. Kim, S. J.; Lee, G. M. Cytogenetic analysis of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 1999, 64, 741-749.
15. Serakinci, N.; Krejci, K.; Koch, J. Telomeric repeat organization-a comparative in situ study between man and rodent. Cytogenet. Cell. Genet. 1999, 86, 204-211.
16. n, present at the telomeres of human chromosomes. Proc. Natl. Acad. Sci. U. S. A. 1988, 85, 6622-6626.
17. Kim, N. S.; Kim, S. J.; Lee, G. M. Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: stability in the absence of selective pressure. Biotechnol. Bioeng. 1998, 60, 679-688.
18. Kim, N. S.; Byun, T. H.; Lee, G. M. Key determinants in the occurrence of clonal variation in humanized antibody expression of CHO cells during dihydrofolate reductase mediated gene amplification. Biotechnol. Prog. 2001, 17, 69-75.
19. Chuck, A. S.; Palsson, B. O. Population balance between producing and nonproducing Hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition. Biotechnol. Bioeng. 1992, 39, 354-360.
20. Balajee, A. S.; Dominguez, I.; Natarajan, A. T. Construction of Chinese hamster chromosome specific DNA libraries and their use in the analysis of spontaneous chromosome rearrangements in different cell lines. Cytogenet. Cell Genet. 1995, 70, 95-101.
21. Ma, C.; Martin, S.; Trask, B.; Hamlin, J. L. Sister chromatid fusion initiates amplification of the dihydrofolate reductase gene in Chinese hamster cells. Genes Dev. 1993, 7, 605-620.
22. Slijepcevic, P.; Xiao, Y.; Dominguez, I.; Natarajan, A. T. Spontaneous and radiation-induced chromosomal breakage at interstitial telomeric sites. Chromosoma 1996, 104, 596-604.
23. Sanders, P. G.; Hussein, A.; Coggins, L.; Wilson, R. Gene amplification: the Chinese hamster glutamine synthetase gene. Dev. Biol. Stand. 1987, 66, 55-63.
24. Brown, M. E.; Renner, G.; Field, R. P.; Hassell, T. Process development for the production of recombinant antibodies using the glutamine synthetase (GS) system. Cytotechnology 1992, 9, 231-236.
25. Cosgrove, L.; Lovrecz, G. O.; Verkuylen, A.; Cavaleri, L.; Black, L. A.; Bentley, J. D.; Howlett, G. J.; Gray, P. P.; Ward, C. W.; McKern, N., M. Purification and properties of insulin receptor ectodomain from large-scale mammalian cell culture. Protein Expression Purif. 1995, 6, 789-798.
26. Wilson, R. H.; Bebbington, C. R. Method for dominant selection in eucaryotic cells. U.S. Patent 5,122,464, 1992.
27. Guarna, M. M.; Fann, C. H.; Busby, S. J.; Walker, K. M.; Kilburn, D. G.; Piret, J. M. Effect of cDNA copy number on secretion rate of activated protein-C. Biotechnol. Bioeng. 1995, 46, 22-27.
28. Cowell, J. K.; Miller, O. J. Occurrence and evolution of homogeneously staining regions may be due to breakage-fusion-bridge cycles following telomere loss. Chromosoma 1983, 88, 216-221.
29. Kaufman, R. J.; Sharp, P. A.; Latt, S. A. Evolution of chromosomal regions containing transfected and amplified dihydrofolate reductase sequences. Mol. Cell. Biol. 1983, 3, 699-711.
30. Gisselsson, D.; Pettersson, L.; Hoglund, M.; Heidenblad, M.; Gorunova, L.; Wiegant, J.; Mertens, F.; Dal Cin, P.; Mitelman, F.; Mandahl, N. Chromosomal breakage-fusion-bridge events cause genetic intratumor heterogeneity. Proc. Natl. Acad. Sci. U. S. A. 2000, 97, 5357-5362.
31. Toledo F., Le Roscouet D., Buttin G., Debatisse M. Co-amplified markers alternate in megabase long chromosomal inverted repeats and cluster independently in interphase nuclei at early steps of mammalian gene amplification. EMBO J. 1992, 11, 2665-2673.
32. Schubert, I.; Oud, J. L. There is an upper limit of chromosome size for normal development of an organism. Cell 1997, 88, 515-520.
33. n sequences in gene amplification and chromosome stability. Genomics 1994, 24, 53-62.
34. Slijepcevic, P.; Bryant, P. E. Absence of terminal telomeric FISH signals in chromosomes from immortal Chinese hamster cells. Cytogenet. Cell. Genet. 1995, 69, 87-89.
35. Harrington, L. A.; Greider, C. W. Telomerase primer specificity and chromosome healing. Nature 1991, 353, 451-454.
36. Morin, G. B. Recognition of a chromosome truncation site associated with alpha-thalassaemia by human telomerase. Nature 1991, 353, 454-456.
37. Meltzer, P. S.; Guan, X. Y.; Trent, J. M. Telomere capture stabilizes chromosome breakage. Nat. Genet. 1993, 4, 252-255.
38. Slijepcevic, P.; Bryant, P. E. Chromosome healing.; telomere capture and mechanisms of radiation-induced chromosome breakage. Int. J. Radiat. Biol. 1998, 73, 1-13.
39. Dunham, M. A.; Neumann, A. A.; Fasching, C. L.; Reddel, R. R. Telomere maintenance by recombination in human cells. Nat. Genet. 2000, 26, 447-450.
40. Li, J. C.; Kaminskas, E. Accumulation of DNA strand breaks and methotrexate cytotoxicity. Proc. Natl. Acad. Sci. U. S. A. 1984, 81, 5694-5698.
41. Nelson, W. G.; Kastan, M. B. DNA strand breaks: the DNA template alterations that trigger p53-dependent DNA damage response pathways. Mol. Cell. Biol. 1994, 14, 1815-1823.
42. Kuo, M. T.; Vyas, R. C.; Jiang, L.; Hittelman, W. N. Chromosome breakage at a major fragile site associated with P-glycoprotein gene amplification in multidrug-resistant CHO cells. Mol. Cell. Biol. 1994, 14, 5202-5211.
43. Coquelle, A.; Pipiras, E.; Toledo, F.; Buttin, G.; Debatisse, M. Expression of fragile sites triggers intrachromosomal mammalian gene amplification and sets boundaries to early amplicons. Cell 1997, 89, 215-225.
44. Bouffler, S. D. Involvement of telomeric sequences in chromosomal aberrations. Mutat. Res. 1998, 404, 199-204.
45. Faravelli, M.; Azzalin, C. M.; Bertoni, L.; Chernova, O.; Attolini, C.; Mondello, C.; Giulotto, E. Molecular organization of internal telomeric sequences in Chinese hamster chromosomes. Gene 2002, 283, 11-16.
46. Hastie, N. D.; Allshire, R. C. Human telomeres: fusion and interstitial sites. Trends Genet. 1989, 5, 326-331.
47. Barranco, S. C.; Shilkun, K.; Nichols, S.; Boerwinkle, W. R.; Adams, E. G.; Bhuyan, B. K. Changes in DNA distributions and ploidy of CHO cells as a function of time in culture. In Vitro 1981, 17, 730-734.
48. Racher, A. J. Alternative approaches to developing highly productive cell culture processes for producing therapeutic antibodies. IBC Life Sciences Conference, La Jolla, CA USA; 2003, March 5-7.